Molecular Monitoring of hrpB-disrupted Mutant of Ralstonia solanacearum in Tomato Plants
スポンサーリンク
概要
- 論文の詳細を見る
A nonpathogenic mutant of Ralstonia solanacearum was produced by the insertion of transposon Tn4431.The mutagenized gene was then cloned from a genomic DNA library by the gene tagging method, using the labeled lux operon located on Tn4431 of pUCD623 as a hybridization probe.From nucleotide sequence analysis of the transposoninserted genomic clone, the hrpB gene was shown to be disrupted by the inserted transposon.Tomato plants were inoculated with the hrpB-disrupted mutant bacteria, for which multiplication and translocation were then monitored using the colony hybridization method.In addition, the original pathogenic bacteria in which the lux operon had been functionally ligated with the genomic promoter were also used for inoculation and traced by their bioluminescence.Multiplication of the hrpB-disrupted mutant was suppressed initially in the invaded root tissues and then in upper hypocotyl after translocation, suggesting that the pathogenic strain of R.solanacearum overcomes at least two steps of host responses expressed in root and hypocotyl tissues.Thus, our approach for molecular monitoring of the bacteria enabled us to precisely analyze the infection behavior of the pathogenic bacteria in planta.
- 日本植物病理学会の論文
- 2000-02-25
著者
-
Nakanishi Tateo
National Agricultural Research Center For Western Region
-
Matsuda Yoshinori
Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University
-
KAKUTANI Koji
Pharmaceutical Research and Technology Institute, Kinki University
-
MATSUDA Yoshinori
Institute for Comprehensive Agricultural Sciences, Kinki University
-
TOYODA Hideyoshi
Faculty of Agriculture, Kinki University
-
KATO Yasunari
Faculty of Agriculture, Kinki University
-
BINGO Miki
Faculty of Agriculture, Kinki University
-
OUCHI Seiji
Faculty of Agriculture, Kinki University
-
Nonomura T
Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University
-
Bingo Miki
Faculty Of Agriculture Kinki University
-
Ouchi S
Department Of Agronomy Faculty Of Agriculture Kinki University
-
Kato Yasunari
Faculty Of Agriculture Kinki University
-
Toyoda Hideyoshi
Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University
-
Matsuda Yoshinori
Pharmaceutical Research And Technology Institute Kinki University
-
KAKUTANI Koji
Faculty of Agriculture, Kinki University
-
NAKANISHI Takayuki
Faculty of Agriculture, Kinki University
-
NONOMURA Teruo
Faculty of Agriculture, Kinki University
-
Nishida Takatsugu
Faculty Of Agriculture Kinki University
-
Kawamura Kiyohisa
Laboratory Of Ecological Entomology Faculty Of Agriculture Kinki University
-
Matsuda Yoshinori
Institute For Comprehensive Agricultural Sciences Kinki University
-
Matsuda Yoshinori
Institute For Comprehensive Agricultural Science Kinki University
関連論文
- Efficient Release of Overproduced Gene Products from Escherichia coli BL21(DE3) by Lytic Infection with Newly Isolated Bacteriophages(Biochemistry & Molecular Biology)
- Evaluation for Efficient Hairy Root Production Assessed by GFP-gene Expression in Tomato Cultivars Inoculated with Agrobacterium rhizogenes MAFF07- 20001
- Foot Rot of Ulluco Caused by Pythium aphanidermatum
- Rhizoctonia Blight of Yacon Caused by Rhizoctonia solani AG-1 (IB)
- Screening of Wild Lycopersicon Species for Resistance to Japanese Isolate of Tomato Powdery Mildew Oidium neolycopersici
- Consecutive monitoring for conidiogenesis by Oidium neolycopersici on tomato leaves with a high-fidelity digital microscope
- Cytological events in tomato leaves inoculated with conidia of Blumeria graminis f. sp. hordei and Oidium neolycopersici KTP-01
- Infectivity of a Japanese isolate of Oidium neolycopersici KTP-01 to a European tomato cultivar resistant to O. lycopersici
- Morphological and molecular characterization for a Japanese isolate of tomato powdery mildew Oidium neolycopersici and its host range
- Induction of Morphologically Changed Petals from Mutagen-treated Apical Buds of Rose and Plant Regeneration from Varied Petal-derived Calli
- 水耕栽培の主要藻類であるクラミドモナス属緑藻の増殖に及ぼすインドール類縁化合物の効果
- Gene Expression in Tomato for Glucosylation of Indole-3-Propionic Acid, a Selective Antibacterial Agent against a Bacterial Wilt Pathogen Pseudomonas solanacearum
- Evaluation of Stable Resistance Expression in Self-pollinated Progenies of Bacterial Wilt Resistant Regenerants Obtained from Leaf Callus of Tomato
- タバコカルスより選抜したTMV抵抗性系統CMT-IR03カルス細胞へのマイクロインジェクション法によるTMVの導入とその増殖ならびに移行
- バラ花弁外植片からのカルス誘導と個体再生
- フザリン酸抵抗性トマトカルス再生体の自殖後代におけるトマト萎ちょう病抵抗性の発現
- Antioxidant constituents in the dayflower (Commelina communis L.) and their α-glucosidase-inhibitory activity
- Rapid Embryogenesis and Plant Regeneration of Target Tissue Derived from Juvenile Shoots for Transformation of Oat by Microprojectile Bombardment
- Growth Inhibition of Pseudomonas solanacearum by Substituted 3-Indolepropionic Acids and Related Compounds
- Indole as an Antibacterial Substance against Pseudomonas solanacearum(Organic Chemistry)
- Family 19 Chitinase from Aeromonas sp. No. 10S-24 : Role of Chitin-Binding Domain in the Enzymatic Activity
- Genetic analysis of Cochliobolus heterostrophus polyoxinresistant mutants
- Chitinous Component of the Cell Wall of Fusarium oxysporum, Its Structure Deduced from Chitosanase Digestion
- Chitinous Components of the Cell Wall of Fusarium oxysporum
- Solid State ^C-NMR Analysis of Cell Wall Components of Fusarium oxysporum(Biological Chemistry)
- Distinguishable staining with neutral red for GFP-marked and GFP-nonmarked Fusarium oxysporum strains simultaneously colonizing root surfaces
- Constituents of Volatile Oil from Melon Hairy Roots Produced by the Infection with Agrobacterium rhizogenes
- A PCR-based method for mating type determination in Cochliobolus heterostrophus
- A Monitoring System for Green Fluorescence Protein Gene-transformed Fusarium oxysporum in Melon Seedlings
- A convenient estimation of the sources of sweet potato weevils, Cylas formicarius(Fabricius)(Coleoptera: Brentidae), in recently invaded areas in Japan, by random amplified polymorphic DNA technique
- Genetic variation of sweet potato weevils, Cylas formicarius(Fabricius)(Coleoptera: Brentidae), in main infested areas in the world based upon the internal transcribed spacer-1(ITS-1)region
- Rapid detection of chitosanase activity in chitosanase gene-transformed strains of Enterobacter cloacae by lytic infection of specific bacteriophages
- Detection of polymorphic patterns of genomic DNA amplified by RAPD-PCR in sweet potato weevils, Cylas formicarius (Fabricius) (Coleoptera : Brentidae)
- In Vitro Suppression of Mycelial Growth of Fusarium oxysporum by Extracellular Chitosanase of Sphingobacterium multivorum and Cloning of the Chitosanase Gene csnSM1
- Occurrence of New Powdery Mildew on Greenhouse Tomato Cultivars
- Susceptibility of Commercial Tomato Cultivars to Bacterial Wilt in Hydroponic System
- Molecular Monitoring of hrpB-disrupted Mutant of Ralstonia solanacearum in Tomato Plants
- Conidia of Erysiphe trifoliorum attempt penetration twice during a two-step germination process on non-host barley leaves and an artificial hydrophobic surface
- Production of Crown-Rot Resistant Strawberry by Concentration of Indigenously Existing Resistant Cells through Tissue Culture
- Somatic Embryogenesis and Plantlet Regeneration on Several Species of Licorice
- In Situ Hybridization Detection of mRNA in a Filamentous Phytopathogenic Fungus, Fusarium oxysporum f.sp. lycopersici, by Pricking Microinjection of Photobiotin-labeled Antisense RNA Probe
- Conidia of Erysiphe trifoliorum attempt penetration twice during a two-step germination process on non-host barley leaves and an artificial hydrophobic surface
- Conidia of the tomato powdery mildew Oidium neolycopersici initiate germ tubes at a predetermined site
- Circulated Culture System of Tobacco Hairy Roots for Rapid Evaluation of Viral Multiplication and Movement
- Production of Crown-Rot Resistant Strawberry by Concentration of Indigenously Existing Resistant Cells through Tissue Culture