Efficient Release of Overproduced Gene Products from Escherichia coli BL21(DE3) by Lytic Infection with Newly Isolated Bacteriophages(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
Overproduced proteins from Escherichia coli BL21(DE3) were efficiently released with virulent bacteriophages. Leviviridae-like bacteriophages were isolated from soil and used to lyse BL21(DE3) cells transformed with β-glucosidase, chitinase, or chitosanase genes. This method caused lysis of bacterial cells similar to that by conventional sonication and enabled us to effectively recover and purify the enzymes.
- 2003-01-23
著者
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Mayama S
Laboratory Of Plant Pathology Graduate School Of Science And Technology Faculty Of Agriculture Kobe
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Saito Ryo
Laboratory Of Food Biochemistry Division Of Applied Bioscience Graduate School Of Agriculture Hokkai
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Matsuda Yoshinori
Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University
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Nonomura Teruo
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University
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Toyoda Hideyoshi
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University
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KAKUTANI Koji
Pharmaceutical Research and Technology Institute, Kinki University
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Nonomura T
Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University
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TOSA Yukio
Laboratory of Plant Pathology, Faculty of Agriculture, Kobe Unoversity
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MAYAMA Shigeyuki
Laboratory of Plant Pathology, Faculty of Agriculture, Kobe Unoversity
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Nonomura Teruo
Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University
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Saito Ryuichiro
Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University
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Tosa Yukio
Fac. Of Agriculture Kobe Univ.
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Tosa Yukio
Laboratory Of Plant Pathology Faculty Of Agriculture Kobe University
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Kakutani Koji
Pharmaceutical Research And Technology Institute Kinki University
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Toyoda Hideyoshi
Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University
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Matsuda Yoshinori
Pharmaceutical Research And Technology Institute Kinki University
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IIDA Yuichiro
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University
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NAKASATO Masanori
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University
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Kawamura Kiyohisa
Laboratory Of Ecological Entomology Faculty Of Agriculture Kinki University
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Ikegami Y
Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University
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Nakasato Masanori
Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University
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Matsuda Yoshinori
Institute For Comprehensive Agricultural Science Kinki University
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