Involvement of gacA Gene in the Suppression of Tomato Bacterial Wilt by Pseudomonas fluorescens FPT9601

スポンサーリンク

概要

• 論文の詳細を見る

• Pseudomonas fluorescens FPT9601, a plant growth-promoting rhizobacterium (PGPR) isolated from tomato rhizosphere, can protect tomato (Lycopersicon esculentum Mill) from bacterial wilt disease caused by Ralstonia solanacearum. This strain produces antibiotics 2,4-diacetylphloroglucinol (2,4-DAPG) and hydrogen cyanide (HCN). It also produces proteases and uncharacterized siderophores (Sid). A mutant strain SM2214, obtained by Tn5 insertion, did not produce 2,4-DAPG, HCN or proteases, but overproduced Sid. Marker-exchange mutagenesis confirmed that a single transposon insertion caused the multiple phenotypic changes of this mutant. Complementation of the mutant with a 1.3-kb DNA fragment that was amplified from genomic DNA of the wild-type P. fluorescens strain by PCR could restore the lost functions of the mutant strain. Nucleotide sequencing revealed that the fragment contained a 642-bp open reading frame (ORF) highly homologous to the regulator responser gene gacA. The in vitro anti-bacterium test and plant protection experiment under greenhouse conditions indicated that the gacA gene played an important role in the suppression of tomato bacterial wilt disease. (Received November 20, 2000 ; Accepted January 19, 2001)

• 2001-05-25

著者

• Mayama S

  Laboratory Of Plant Pathology Graduate School Of Science And Technology Faculty Of Agriculture Kobe

• TOSA Yukio

  Laboratory of Plant Pathology, Faculty of Agriculture, Kobe Unoversity

• NAKAYASHIKI Hitoshi

  Laboratory of Plant Pathology, Faculty of Agriculture, Kobe Unoversity

• MAYAMA Shigeyuki

  Laboratory of Plant Pathology, Faculty of Agriculture, Kobe Unoversity

• Yang Qian

  Laboratory Of Plant Pathology Division Of Environmental Science Of Bioresource Production Graduate S

• Tosa Yukio

  Fac. Of Agriculture Kobe Univ.

• Tosa Yukio

  Laboratory Of Plant Pathology Faculty Of Agriculture Kobe University

• ZHANG Liqun

  Laboratory of Plant Pathology, Division of Environmental Science of Bioresource Production, Graduate

• Zhang Liqun

  Dept. Of Plant Pathology China Agricultural University

• NAKAYASHIKI Hitoshi

  Faculty of Agriculture, Kobe University

• Nakayashiki Hitoshi

  Faculty Of Agriculture Kobe University

• Nakayashiki Hitoshi

  Laboratory Of Plant Pathology Graduate School Of Agriculture Kyoto University

• Nakayashiki Hitoshi

  Laboratory Of Plant Pathology Graduate School Of Agricultural Science Kobe University

関連論文

• Efficient Release of Overproduced Gene Products from Escherichia coli BL21(DE3) by Lytic Infection with Newly Isolated Bacteriophages(Biochemistry & Molecular Biology)
• Victorin Triggers Programmed Cell Death and the Defense Response via Interaction with a Cell Surface Mediator
• RNA silencing as a tool for exploring gene function in ascomycete fungi
• OARE-1, a Ty1-copia Retrotransposon in Oat Activated by Abiotic and Biotic Stresses
• Involvement of gacA Gene in the Suppression of Tomato Bacterial Wilt by Pseudomonas fluorescens FPT9601
• Pathogenicity, Mating Ability and DNA Restriction Fragment Length Polymorphisms of Pyricularia Populations Isolated from Gramineae, Bambusideae and Zingiberaceae Plants
• Cauliflower Mosaic Virus ORF III Product Forms a Tetramer In Planta:Its Implication in Viral DNA Folding during Encapsidation
• Requirement of Cauliflower Mosaic Virus Open Reading Frame VI Product for Viral Gene Expression and Multiplication in Turnip Protoplasts
• Evidence for a Dual Strategy in the Expression of Cauliflower Mosaic Virus Open Reading Frames I and IV
• Accumulation Kinetics of Viral Gene Products in Cauliflower MOsaic Virus-Infected Turnip Protoplasts
• Rapid detection of chitosanase activity in chitosanase gene-transformed strains of Enterobacter cloacae by lytic infection of specific bacteriophages
• Occurrence of New Powdery Mildew on Greenhouse Tomato Cultivars
• Nitric oxide-overproducing transformants of Pseudomonas fluorescens with enhanced biocontrol of tomato bacterial wilt
• Induction of Chlorosis, ROS Generation and Cell Death by a Toxin Isolated from Pyricularia oryzae
• Current Status of Biological Control of Plant Diseases in China
• Two phases of intracellular reactive oxygen species production during victorin-induced cell death in oats
• Signal Mediators for Phytoalexin Production in Defense Response of Oats Elicited by Victorin as a Specific Elicitor
• Rwt4, a wheat gene for resistance to Avena isolates of Magnaporthe oryzae, functions as a gene for resistance to Panicum isolates in Japan
• Reduction in aggressiveness among hybrids between host-specific pathotypes of Magnaporthe oryzae is caused by reduced ability to overcome adult resistance at the level of penetration
• Significance of PWT4-Rwt4 interaction in the species specificity of Avena isolates of Magnaporthe oryzae on wheat
• Oat Retrotransposon OARE-1 Is Activated in Both Compatible and Incompatible Interactions with Pathogenic Fungi
• Heat shock, copper sulfate and oxidative stress activate the retrotransposon MAGGY resident in the plant pathogenic fungus Magnaporthe grisea
• Speciation in Pyricularia inferred from multilocus phylogenetic analysis
• Evolution and diversification of RNA silencing proteins in fungi
• RNA silencing in fungi: Mechanisms and applications
• Conidia of Erysiphe trifoliorum attempt penetration twice during a two-step germination process on non-host barley leaves and an artificial hydrophobic surface
• Conidia of the tomato powdery mildew Oidium neolycopersici initiate germ tubes at a predetermined site
• The C-terminal chromodomain-like module in the integrase domain is crucial for high transposition efficiency of the retrotransposon MAGGY
• Degenerate MAGGY elements in a subgroup of Pyricularia grisea: a possible example of successful capture of a genetic invader by a fungal genome
• Novel vectors for retrotransposon-induced gene silencing in Magnaporthe oryzae
• Cytological characteristics of microconidia of Magnaporthe oryzae
• Population structure of Eleusine isolates of Pyricularia oryzae and its evolutionary implications
• Phylogenetic analyses of plant-growth-promoting rhizobacteria isolated from tomato, lettuce, and Japanese pepper plants in Hyogo Prefecture, Japan
• An avirulence gene to rice cultivar K60 is located on the 1.6-Mb chromosome in Magnaporthe oryzae isolate 84R-62B
• Taxonomic characterization of Pyricularia isolates from green foxtail and giant foxtail, wild foxtails in Japan
• Population structure of rice blast isolates resistant to scytalone dehydratase inhibitors in Mie Prefecture and implications for their origin
• Instability of subtelomeric regions during meiosis in Magnaporthe oryzae
• Characterization of interactions between barley and various host-specific subgroups of Magnaporthe oryzae and M. grisea

もっと見る 閉じる

スポンサーリンク