Induction of Chlorosis, ROS Generation and Cell Death by a Toxin Isolated from Pyricularia oryzae
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概要
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The ethyl acetate extract of the conidia germination fluid from an Avena isolate (Br58) of Pyricularia oryzae had chlorosis-inducing activity on oat leaf segments. The same activity was also present in the acetone extract of an oatmeal agar culture of Br58. Fungal cultures were used for a large-scale preparation. A series of acetone and ethyl acetate extraction monitored by chromatography was used to isolate an active fraction. The active principle was purified by HPLC. We show by NMR and LC/MS that the toxin was an oxidized C18 unsaturated fatty acid named Mag-toxin. Mag-toxin induced chlorosis on oat leaf segments incubated in the light but not in the dark. Reactive oxygen species (ROS) and cell death were induced by Mag-toxin in oat cells. The sub-cellular localization of ROS generation induced by the toxin treatment was correlated with the location of mitochondria. Interestingly, the induction of ROS generation and cell death by Mag-toxin was light-independent.
- 2010-11-23
著者
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Mayama S
Laboratory Of Plant Pathology Graduate School Of Science And Technology Faculty Of Agriculture Kobe
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Mayama Shigeyuki
Faculty of Agriculture, Kobe University
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Tosa Yukio
Fac. Of Agriculture Kobe Univ.
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Tosa Yukio
Laboratory Of Plant Pathology Faculty Of Agriculture Kobe University
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Miyagawa Hisashi
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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TSURUSHIMA Tetsu
Faculty of Business, Hannan University
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MINAMI Yukari
Faculty of Agriculture, Kobe University
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NAKAYASHIKI Hitoshi
Faculty of Agriculture, Kobe University
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TOSA Yukio
Faculty of Agriculture, Kobe University
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Tsurushima Tetsu
Faculty Of Business Hannan University
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Mayama Shigeyuki
Faculty Of Agriculture Kobe University
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Nakayashiki Hitoshi
Faculty Of Agriculture Kobe University
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Minami Yukari
Faculty Of Agriculture Kobe University
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