Purification and Characterization of Chitinase B from Moderately Thermophilic Bacterium Ralstonia sp. A-471
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概要
- 論文の詳細を見る
Chitinase B was purified from a culture medium of Ralstonia sp. A-471 by precipitation with (NH4)2SO4 and column chromatography with DEAE-Toyopearl 650M and Sephacryl S-200. The purified enzyme was homogeneous on SDS–PAGE. The molecular weight was 45,000 by SDS–PAGE. The optimum pH was 5.0 and stable pH was from 5.0 to 10.0. In the early stage of the reaction, chitinase B produced β-anomer of (GlcNAc)2 from the substrate (GlcNAc)6, whereas (GlcNAc)4 produced almost at equilibrium, indicating that the enzyme predominantly hydrolyzes the second glycosidic linkage from the nonreducing end of (GlcNAc)6.
- 社団法人 日本農芸化学会の論文
- 2005-04-23
著者
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Sutrisno Aji
阪府大院・生命環境科学
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Sutrisno Aji
Laboratory Of Biocycle Engineering Department Of Applied Biological Chemistry Osaka Prefecture Unive
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Sutrisno Aji
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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Miyatake K
Osaka Prefecture Univ. Osaka Jpn
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SUTRISNO Aji
阪府大院農・応生化
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Kirihata Mitsunori
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Osaka Prefecture
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切畑 光統
大阪府立大学 農学部
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Kirihata Mitsunori
Graduate School Of Life And Environmental Science Osaka Prefecture University
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Ueda M
Laboratory Of Biocycle Engineering Department Of Applied Biological Chemistry Osaka Prefecture Unive
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Ueda Mitsuhiro
Laboratory Of Biocycle Engineering Graduate School Of Life And Environmental Sciences Osaka Prefectu
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KOTANI Yukiko
Laboratory of Biocycle Engineering, Department of Applied Biological Chemistry, Osaka Prefecture Uni
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NAKAZAWA Masami
Laboratory of Biocycle Engineering, Department of Applied Biological Chemistry, Osaka Prefecture Uni
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MIYATAKE Kazutaka
Laboratory of Biocycle Engineering, Department of Applied Biological Chemistry, Osaka Prefecture Uni
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Miyatake Kazutaka
Laboratory Of Biocycle Engineering Graduate School Of Life And Environmental Sciences Osaka Prefectu
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Miyatake Kazutaka
Nutrition Laboratory College Of Agriculture
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Nakazawa Masami
Laboratory Of Biocycle Engineering Graduate School Of Life And Environmental Science Osaka Prefectur
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Konishi Yasuhito
Laboratory Of Food Microbiological Science And Biotechnology School Of Agriculture Kin-ki University
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Ueda Mitsuhiro
Laboratory Of Biocycle Engineering College Of Agriculture Osaka Prefecture University
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