Cloning and Sequence Analysis of a Chitinase Gene(pCA8 ORF)from Aeromonas sp.No.10S-24
スポンサーリンク
概要
- 論文の詳細を見る
A gene encoding chitinase from Aeromonas sp.no.10S-24 was cloned into Escherichia coli DH5α using pUC19 and its nucleotide sequence was determined. The pCA8 ORF(open reading frame) consisted of 1482 bp encoding 494 amino acid residues. The molecular weight of the unprocessed protein was calculated to be 51, 441. The pCA8 ORF is a modular enzyme composed of a signal sequence, chitin binding domains, Pro and/or Thr-rich domains, and a family 18 catalytic domain responsible for chitinase activity. The amino acid sequence of pCA8 ORF shares sequence homology with chitinase II(30%)from Aeromonas sp.no.10S-24, and chitinase(22.9%)from Saccharopolyspora erythraea. Since no chitinase corresponding to the pCA8 ORF has been found in Aeromonas sp.no.10S-24 culture supernatants, the gene may be silent in this strain.
- 社団法人日本生物工学会の論文
- 1998-12-25
著者
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KAWAGUCHI Takashi
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring
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Arai Motoo
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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UEDA MITSUHIRO
Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University
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Kawaguchi Takashi
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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Kawaguchi Takashi
College Of Agriculture University Of Osaka Prefecture
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Kawaguchi Takashi
Department Of Drug Safety Research Eisai Co. Ltd.
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Arai M
Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University
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Arai Motoo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Ueda M
Laboratory Of Biocycle Engineering Department Of Applied Biological Chemistry Osaka Prefecture Unive
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Ueda Mitsuhiro
Laboratory Of Biocycle Engineering Graduate School Of Life And Environmental Sciences Osaka Prefectu
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Kawaguchi T
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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OKADA AYUKO
Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University
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Okada Ayuko
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied
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Ueda Mitsuhiro
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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