Structure of Fucogalactomannan of Red Yeast Cell Wall

概要

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Fucogalactomannan was obtained from the walls of Rhodotorula glutinis K-24 by alkaline extraction, pronase digestion, and alcohol precipitation. This purified polysaccharide was composed of fucose, galactose and mannose in the molar ratio of 1:2.5:2, and occupied about 20% of the cell wall. In the successive Smith degradation, mannose residues were resistant to periodate oxidation, whereas most of galactose residues were degraded, releasing threitol and arabinose. It appeared that mannose residues consisted of a backbone of the polysaccharide and all or part of the galactose residues might be of a furanose type. By partial acid hydrolysis of fucogalactomannan, three kinds of oligosaccharide containing fucose and galactose were obtained. Their structures were identified as Galf(1→6)Galf, Fuc(1→2)Galf(1→2)Galf(1→6)Galf, and Fuc(1→2)Galf(1→6)Galf-(1→2)Galf(1→6)Galf, respectively. The methylation analysis revealed that the polysaccharide consisted of (1→3)-linked mannose residues, all of which were branched at the C-2 position with fucose and galactose residues. Most of the fucose residues existed in the nonreducing end of the side chain. Galactose residues were mostly contained as (1→2) and (1→6) linkages in the side chain.
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