Cloning, Nucleotide Sequence, and Expression of the Clostridium thermocellum Cellodextrin Phosphorylase Gene and Its Application to Synthesis of Cellulase Inhibitors
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概要
- 論文の詳細を見る
The cellodextrin phosphorylase(CDP)gene of Clostridium thermocellum was cloned and sequenced. The nucleotide sequence of the insert of a positive clone contained an open reading frame of 2940 bp encoding a polypeptide of 980 amino acid residue with a calculated molecular mass of 111, 182 daltons. Escherichia coli cells harboring the plasmid for expression produced CDP protein accounting for 30% of the total cellular proteins with an activity of 59.9 units/ml culture, which corresponded to 0.93 mg/ml culture. The expressed COP could be used to synthesize cellulase inhibitors as cellooligosaccharide analogues using glucose-1-phospbte as a glucose donor and 4-O-β-D-glucopyranosyl-1-deoxynojirimycin or 6-O-β-cellobiosyl-1-deoxy nojirimycin as an acceptor.
- 社団法人日本生物工学会の論文
- 1998-02-25
著者
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KAWAGUCHI Takashi
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring
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Arai Motoo
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Kawaguchi Takashi
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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Kawaguchi Takashi
College Of Agriculture University Of Osaka Prefecture
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Kawaguchi Takashi
Department Of Drug Safety Research Eisai Co. Ltd.
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Arai M
Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University
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Arai Motoo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Kawaguchi T
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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SUMITANI Jun-ichi
Department of Applied Biological Chemistry, College of Agriculture, and Research Institute for Advan
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IKEUCHI YASUO
Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University
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TSUTSUMI NORIKO
Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University
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KAN AKIHIKO
Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University
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Sumitani J
Graduate School Of Life And Environmental Sciences Osaka Prefecture University
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Sumitani Jun-ichi
Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied
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Ikeuchi Yasuo
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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Tsutsumi Noriko
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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Kan Akihiko
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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