Molecular Cloning and Nucleotide Sequence of the Gene Encoding Chitinase II from Aeromonas sp. No. 10S-24
スポンサーリンク
概要
- 論文の詳細を見る
A gene encoding chitinase II from Aeromonas sp. no. 10S-24 was cloned into Escherichia coli DH5 α by using pUC19 ans its nucleotides were sequenced. The structural gene consisted of 1626 bp encoding 542 amino acid residues with a characteristic signal peptide. A typical promoter sequence and Shine-Dalgarno (SD) region were located upstream of the initiation ATG codon. The deduced amino acid sequence of the cloned chitinase II showed sequence homology with chitinase from Saccharopolyspora erythraea (26%). The chitinase II had Pro-Thr-rich domains, consisting of repeat sequences of 47 amino acids, that are not conserved in other chitinases from bacteria. Generally, repeat sequence of amino acids rich in Pro and /or Thr appear to link discrete functional domains in many cellulases and xylanases.
- 社団法人日本生物工学会の論文
- 1994-09-25
著者
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KAWAGUCHI Takashi
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring
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Arai Motoo
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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UEDA MITSUHIRO
Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University
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Kawaguchi Takashi
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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Kawaguchi Takashi
College Of Agriculture University Of Osaka Prefecture
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Kawaguchi Takashi
Department Of Drug Safety Research Eisai Co. Ltd.
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Arai M
Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University
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Arai Motoo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Ueda M
Laboratory Of Biocycle Engineering Department Of Applied Biological Chemistry Osaka Prefecture Unive
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Ueda Mitsuhiro
Laboratory Of Biocycle Engineering Graduate School Of Life And Environmental Sciences Osaka Prefectu
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Kawaguchi T
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied
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Ueda Mitsuhiro
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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