Purification and Properties of Cellodextrin Phosphorylase from Clostridium thermocellum
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概要
- 論文の詳細を見る
Cellodextrin phosphorylase [EC 2.4.1.49] was purified 129-fold, with a yield of 22.9%, to electrophoretic and column chromatographic homogeneity from a cell extract of Clostridium thermocellum ATCC 27405 by a procedure which inducred streptomycin treatment, ammonium sulfate precipitation, DEAE-Totopearl 650 M, and Toyopearl HW-55F column chromatography. The molecular weight of the enzyme was estimated to be 200,000 by gel filtration and 105,000 by SDS-PAGE, suggesting that it consisted of two identical subunits. It was suggested by spectrophotometric and chemical analysis that the enzyme contained no pyridoxal 5'-phosphate. The enzyme was inactivated by N-ethylmaleimide and activated by dithiothretiol, inducating that the exposed thiol group(s) was important for the enzymatic activity. The enzyme could synthesize at least cellotriose, cellotetraose, and cellopentaose as deterctable cellodextrins, showing that it might possibly be a good tool for the synthesis of cellodextrins.
- 公益社団法人日本生物工学会の論文
- 1994-03-25
著者
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Kawaguchi Takashi
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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Kawaguchi Takashi
College Of Agriculture University Of Osaka Prefecture
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Kawaguchi Takashi
Department Of Drug Safety Research Eisai Co. Ltd.
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Tanaka K
Univ. East Asia Yamaguchi Jpn
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Arai M
Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University
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Arai Motoo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Kawaguchi T
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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Tanaka Keijitsu
Department Of Bio-resource Science Faculty Of Agriculture Tottori University
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied
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