Cloning and Secretive Expression of the Gene Encoding the Proteinaceous α-Amylase Inhibitor Paim from Streptomyces corchorusii
スポンサーリンク
概要
- 論文の詳細を見る
A gene encoding the proteinaceous α-amylase inhibitor Paim was cloned and sequenced. Southern analysis and the amino acid sequence deduced from the cloned gene indicated that Paim isoforms were encoded in the same gene. When the gene was expressed in Escherichia coli and Streptomyces lividans, recombinant Paim inhibitors were produced in the periplasmic space and in the culture supernatant, respectively. The purified inhibitors had different N-terminal sequences from those of the authentic inhibitors.
- 公益社団法人日本生物工学会の論文
- 2000-08-25
著者
-
Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
-
Sumitani Jun-ichi
Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied
-
Tsujimoto Yoshimasa
Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied
-
Arai Mtoo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
-
Arai Mtoo
Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied
-
Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied
関連論文
- KEY PARAMETERS OF SPERM MOTION IN RELATION TO MALE FERTILITY IN RATS GIVEN α-CHLOROHYDRIN OR NITROBENZENE
- Expression of a Gene Encoding Chitinase(pCA 8 ORF)from Aeromonas sp.No.10S-24 in Escherichia coli and Enzyme Characterization
- Cloning and Sequencing of an Exoglucanase Gene from Streptomyces sp. M23, and Its Expression in Streptomyces lividans TK-24(Genetics, Molecular Biology, and Gene Engineering)
- Cloning and Transcription Analysis of the Aspergillus aculeatus No. F-50 Endoglucanase 2 (cmc2) Gene(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- EFFECTS OF REPEATED DOSES OF COMPOUND E FOR 4 AND 9 WEEKS ON THE MALE REPRODUCTIVE ORGANS (<SPECIAL ISSUE>TESTICULAR TOXICITY)
- EFFECTS OF A DAILY FEEDING-RESTRICTION ON THE REPRODUCTIVE AND DEVELOPMENT TOXICITY PARAMETERS IN FEMALE RATS
- Cloning, Nucleotide Sequence and Expression of the β-N-Acetyl-glucosaminidase Gene from Aeromonas sp.No.10S-24
- Molecular Cloning and Nucleotide Sequence of the β-Galactosidase Gene from Enterobacter cloacae GAO
- Purification and Characterization of β-Galactosidase from Enterobacter cloacae GAO
- Distribution of the FoToml gene encoding tomatinase in formae speciales of Fusarium oxysporum and identification of a novel tomatinase from F. oxysporum f. sp. radicis-lycopersici, the causal agent of Fusarium crown and root rot of tomato
- Cloning and Sequencing of an Endoglucanase Gene from Scopulariopsis brevicaulis TOF-1212,and Its Expression in Saccharomyces cerevisiae
- Cloning and Sequence Analysis of a Chitinase Gene(pCA8 ORF)from Aeromonas sp.No.10S-24
- Expression of the Chitinase III Gene of Aeromonas sp. No. 10S-24 in Escherichia coli
- Purification and Some Properties of Six Chitinases from Aeromonas sp. No. 10S-24
- Molecular Cloning and Nucleotide Sequence of the Gene Encoding Chitinase II from Aeromonas sp. No. 10S-24
- Secretive Expression of the Aspergillus aculeatus Cellulase (FI-CMCase) by Saccharomyces cerevisiae
- Purification and Propeties of Cellobiose Phosphorylase from Clostridium thermocellum
- Expression of the Cellulase (FI-CMCase) Gene of Aspergillus aculeatus in Saccharomyces cerevisiae
- Expression of the Cellulase (FI-CMCase) Gene of Aspergillus aculeatus in Escherichia coli
- New Proteinacens α-Amylase inhibitor (T-76) from Streptomyces nitrosporeus
- Enzymatic Properties of Two Avicelases from Aspergillus aculeatus No. F-50(Biological Chemistry)
- Development of a Homologous Transformation System for Aspergillus aculeatus Based on the sC Gene Encoding ATP-Sulfurylase
- Alteration of Substrate Specificity of Fructosyl-Amino Acid Oxidase from Ulocladium sp. JS-103(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Transformation of Aspergillus aculeatus Using the Drug Resistance Gene of Aspergillus oryzae and the pyrG Gene of Aspergillus nidulans(Microbiology & Fermentation Technology)
- Overexpression of Aspergillus aculeatus Cellobiohydrolase I in Aspergillus oryzae(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Overexpression and Purification of Aspergillus aculeatus β-Mannosidase and Analysis of the Integrated Gene in Aspergillus oryzae
- The Conserved Tryptophan-Arginine-Tyrosine Motif of a Proteinaceous α-Amylase Inhibitor T-76 from Streptomyces nitrosporeus Is Important for Inhibition of Animal α-Amylases but not for an α-Amylase from Bacillus sp.No.195
- Cloning and Sequencing of β-Mannosidase Gene from Aspergillus aculeatus No. F-50
- Expression of Aspergillus aculeatus No. F-50 Cellobiohydrolase I (cbhI) and β-glucosidase 1 (bgl1) genes by Saccharomyces cerevisiae
- Bacillus Animal Type α-Amylase : Cloning and Sequencing of the Gene, and Comparison of the Deduced Amino Acid Sequence with That of Other Amylases
- Cloning, Nucleotide Sequence, and Expression of the Clostridium thermocellum Cellodextrin Phosphorylase Gene and Its Application to Synthesis of Cellulase Inhibitors
- Cloning, Nucleotide Sequence, and Transcriptional Analysis of Aspergillus aculeatus No.F-50 Cellobiohydrolase I(cbhI)Gene
- Molecular Cloning and Expression of Proteinaceous α-Amylase Inhibitor Gene from Streptomyces nitrosporeus in Escherichia coli
- Purification and Some Properties of a Haim-sensitive α-Amylase from Newly Isolated Bacillus sp. No. 195
- Identification of the Microorganism Which Produces Insecticidal Indole Alkaloids, Okaramines A and B(Microbiology & Fermentation Industry)
- Steroid Withdrawal Based on Lymphocyte Sensitivity to Endogenous Steroid in Renal Transplant Recipients
- Purification and Properties of Cellodextrin Phosphorylase from Clostridium thermocellum
- Okaramine B, an Insecticidal Indole Alkaloid, Produced by Penicillium simplicissimum AK-40(Organic Chemistry)
- PA34 INSECTICIDAL INDOLE ALKALOIDS, OKARAMINE A AND B, PRODUCED BY PENICILLIUM SIMPLICISSIMUM
- Okaramine A, a Novel Indole Alkaloid with Insecticidal Activity, from Penicillium simplicissimum AK-40(Organic Chemistry)
- Cloning and Secretive Expression of the Gene Encoding the Proteinaceous α-Amylase Inhibitor Paim from Streptomyces corchorusii
- Photoinduced and Thermally-Induced Bleachings of Amorphous Ge-S Film : Condensed Matter
- Ionic Conductivity of AgI Film on the SiO_2 Glass Substrate
- Determinants Affecting Quality of Life: Implications for Pharmacist Counseling for Patients with Breast Cancer in Japan
- Ionic Conductivity of AgI Film on the SiO2 Glass Substrate