Purification and Some Properties of a Haim-sensitive α-Amylase from Newly Isolated Bacillus sp. No. 195
スポンサーリンク
概要
- 論文の詳細を見る
Newley isolated Bacillus sp. No. 195 produced an extracellular α-amylase sensitive to Haim which was found to inhibit specifically animal α-amylases. The enzyme was purified easily by two steps of starch adsorption and gel filtration using Sephacryl S-200. The purified enzyme, which showed a single band on native-PAGE or SDS-PAGE, had a molecular weight of 60,000 as judged on SDS-PAGE. The optimum pH value for activity and the isoelectric point were around 7.0 and 4.5,respectively. The sensitivity of the amylase to Haim was similar to that of animal amylase rather than bacterial amylase. It was suggested that a Haim-amylase complex might be formed at the molar ratio of 1 : 1. The amino acid sequence F-S-W similar to the triplet F-E-W highly conserved among cry-amylases sensitive to proteinaceous inhibitors, such as Hoe 467-A or Haim, was found in the amino-terminal part of the No. 195 amylase.
- 社団法人日本農芸化学会の論文
- 1992-11-23
著者
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KAWAGUCHI Takashi
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring
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Murao Sawao
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Arai Motoo
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Murao S
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Murao S
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Kawaguchi Takashi
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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Kawaguchi Takashi
College Of Agriculture University Of Osaka Prefecture
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Kawaguchi Takashi
Department Of Drug Safety Research Eisai Co. Ltd.
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Arai M
Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University
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Arai Motoo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Murao S
Sojo Univ. Kumamoto Jpn
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Murao Sawao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Kawaguchi T
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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NAGAE HISATO
Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied
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Nagae Hisato
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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