A New Microorganism Producing a Glucosyl Transfer Enzyme to Polyphenols
スポンサーリンク
概要
- 論文の詳細を見る
A microorganism producing a glucosyl transfer enzyme to hydroquinone was isolated from soil and identified as Bacillus subtilis according to its taxonomical characteristics. The enzyme (GSase) was puri-fied from the culture filtrate by column chromatographies, including affinity chromatography using Amylostatin-immobilized Sepharose 4B. The final preparation showed a single band on SDS polyacrylamide gel electrophoresis, the molecular weight being 67kDa. Its optimum pH for starch dextrinization was 7, while that for glucosyl transferring activity was 6, pH stability was 5-8, and isoelectric point was 5.1. GSase was not activated by Ca^<2+>. It used malto-oligosaccharides and dextrin as well as soluble starch more effectively than maltose as glucose donors. It did not catalyze cyclodextrination from starch. GSase glucosylated various polyphenols, such as dihydroxy benzenes, hydroxy benzyl alcohols, phloroglucinol, (+)catechin, kojic acid, dihydroxy benzoic acids, caffeic acid, and gallic acid.
- 社団法人日本農芸化学会の論文
- 1994-05-23
著者
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Murao Sawao
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Murao S
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Murao S
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Morikawa Shin-ya
School Of Pharmaceutical Sciences Nagasaki University
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SHIN Takashi
Department of Applied Microbial Technology, The Kumamoto Institute of Technology
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Murao S
Sojo Univ. Kumamoto Jpn
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Murao Sawao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Shin T
Department Of Applied Microbial Technology The Kumamoto Institute Of Technology
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Shin Takashi
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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YAMAMOTO Ryohei
Kurabo Industries Limited
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Funayama Masataka
Technical Research Laboratory, Kurabo Industries Ltd.
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Arakawa Hirokuni
Technical Research Laboratory, Kurabo Industries Ltd.
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Yamamoto Ryohei
Technical Research Laboratory, Kurabo Industries Ltd.
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Nishino Toyokazu
Technical Research Laboratory, Kurabo Industries Ltd.
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Nishino T
Technical Research Laboratory Kurabo Industries Ltd.
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Arakawa Hirokuni
Technical Research Laboratory Kurabo Industries Ltd.
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Takenisi Shigeyuki
Osaka Municipal Technical Research Institute
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Funayama Masataka
Technical Research Laboratory Kurabo Industries Ltd.
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MURAO SAWAO
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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SHIN Takashi
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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