Streptomyces Pepsin Inhibitor-Insensitive Carboxyl Proteinase from Lentinus edodes
スポンサーリンク
概要
- 論文の詳細を見る
A Streptomyces-pepsin inhibitor (S-PI or Pepstatin Ac), and DAN-insensitive carboxyl proteinase was found in a still culture filtrate of Lentinus edodes. The new carboxyl proteinase was purified, and about 9 mg purified enzyme was obtained from 19 liters of culture filtrate, with 11 % recovery. The enzyme showed a single band on polyacrylamide gel electrophoresis. The molecular weight and isoelectric point were 40, 000 and pH 4.2, respectively. The enzyme did not contain histidine and was composed of 387 amino acid residues. The enzyme was most active between pH 2.72.9, and stable over a pH of 3.2-5.2 for 3 hr at 37°C. The enzyme was not inhibited by S-PI or synthetic pepsin inhibitors such as DAN and EPNP. The physicochemical and enzymological properties were very similar to those of Scytalidium lignicolum carboxyl proteinase A, which was reported to be an S-PI-, and DAN-insensitive carboxyl proteinase.
- 社団法人 日本農芸化学会の論文
著者
-
ODA Kohei
Department of Applied Biology, Kyoto Institute of Technology
-
Kono M
Fisheries Rsearch Laboratory Faculty Of Agriculture The University Of Tokyo
-
TERASHITA Takao
Department of Food and Nutrition, Faculty of Agriculture, Kinki University
-
Murao Sawao
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
-
KONO Matashi
Department of Food and Nutrition, College of Agriculture, Kinki University
関連論文
- Cochinin B, a Novel Ribosome-Inactivating Protein from the Seeds of Momordica cochinchinensis(Biochemistry)
- An Alkaline Serine-Proteinase from a Bacterium Isolated from Bat Feces: Purification and Characterization
- A possible role of the key enzymes of the glyoxylate and gluconeogenesis pathways for fruit-body formation of the wood-rotting basidiomycete Flammulina velutipes
- High Molecular Weight Transglutaminase Inhibitor Produced by a Microorganism (Streptomyces lavendulae Y-200)
- Acetoxydehydroaustin, a New Bioactive Compound, and Related Compound Neoaustin from Penicillium sp. MG-11
- Purification and Some Properties of an Inhibitor for a Yeast Pleiotropic Drug Resistant Pump from Kitasatospora sp. E-420(Biochemistry & Molecular Biology)
- A Novel Screening for Inhibitors of a Pleiotropic Drug Resistant Pump, Pdr5, in Saccharomyces cerevisiae(Biochemistry & Molecular Biology)
- Horseradish Peroxidase Degrades Lipid Hydroperoxides and Suppresses Lipid Peroxidation of Polyunsaturated Fatty Acids in the Presence of Phenolic Antioxidants(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Identification of a novel cuticular protein in the kuruma prawn Penaeus japonicus
- Elevation of the activities of β-N-acetylhexosaminidase isozymes by 20-hydroxyecdysone administration in the kuruma prawn
- Identification of the Microorganism Which Produces Epstein-Barr Virus Early Antigen-inducing Indole Alkaloids
- Purification and Characterization of β-N-Acetylhexosaminidase from the Liver of a Prawn, Penaeus japonicus
- Chitinolytic Enzyme Activities in the Hepatopancresas, Tail Fan and Hemolymph of Kuruma Prawn Penaeus japonicus during the Molt Cycle
- Kinetics of a Chitinase from a Prawn, Penaeus japonicus(Biological Chemistry)
- Purifications and Some Properties of Chitinase from the Liver of a Prawn, Penaeus japonicus(Biological Chemistry)
- Purification and Some Properties of Chitinase from the Stomach of Japanese Eel, Anguilla japonica(Biological Chemistry)
- Streptomyces Pepsin Inhibitor-insensitive Carboxyl Proteinase from Ganoderma lucidum
- Streptomyces Pepsin Inhibitor-Insensitive Carboxyl Proteinase from Lentinus edodes
- Purification and Some Properties of Carboxyl Proteinase in Mycelium of Lentinus edodes
- Molecular Cloning of the Gene Encoding Thermostable Endo-1,5-α-L-arabinase of Bacillus thermodenitrificans TS-3 and Its Expression in Bacillus subtilis(Biochemistry & Molecular Biology)
- Molecular Cloning, DNA Sequence, and Expression of the Gene Encoding for Thermostable Pectate Lyase of Thermophilic Bacillus sp. TS 47
- Purification and Characterization of Thermostable Pectate Lyase with Protopectinase Activity from Thermophilic Bacillus sp. TS 47
- Purification and Characterization of an Endo-Polygalacturonase from Aspergillus awamori
- Catalytic Residues and Substrate Specificity of Recombinant Human Tripeptidyl Peptidase I (CLN2)
- A CLN2-Related and Thermostable Serine-Carboxyl Proteinase, Kumamolysin: Cloning, Expression, and Identification of Catalytic Serine Residue
- Subsite Preferences of Pepstatin-Insensitive Carboxyl Proteinases from Prokaryotes: Kumamolysin, a Thermostable Pepstatin-Insensitive Carboxyl Proteinase^1
- Nucleotide Sequence of the Gene Encoding Pepstatin-insensitive Acid Protease B, Scytalidopepsin B, of Scytalidium lignicolum
- A Novel Laccase Inhibitor, N-Hydroxyglycine, Produced by Penicillium citrinum YH-31
- New Enzymatic Colorimetric Analyses to Evaluate the Activities of Carboxypeptidase A and B(Analytical Chemistry)
- A New Screening Method for Microorganisms Which Produce Carboxypeptidases
- New Enzymatic Colorimetric Reactions of Benzoic Acid Derivatives with ABTS [2, 2-Azino-di-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] in the Presence of Laccase
- Purification and Some Properties of Urethane Hydrolase II from Lactobacillus casei ε ATCC 7469
- Isolation and Characterization of N^α-Benzyloxycarbonyl Amino Acid Urethane Hydrolase II from Lactobacillus fermenti 36 ATCC 9338(Biological Chemistry)
- Structures of the Laccase-catalyzed Oxidation Products of Hydroxy-benzoic Acids in the Presence of ABTS [2,2'-Azino-di-(3-ethylbenzothiazoline-6-sulfonic Acid)]
- A Simultaneous Assay Method for L-Glutamate and L-Pyroglutamate Contents in Soy Sauce Using a 5-Oxoprolinase (without ATP Hydrolyzing Activity)
- Nucleotide Sequence of the Gene Encoding the Precursor Protein of Pepstatin insensitive Acid Protease B, Scytalidopepsin B, from Scytalidium lignicolum
- Purification and Characterization of a Prolyl Endopeptidase from Pseudomonas sp. KU-22
- Isolation and Characterization of a Novel 5-Oxoprolinase (without ATP-hydrolyzing)from Alcaligenes faecalis N-38A
- Isolation and Characterization of a Dipeptidyl Aminopeptidase from Streptomyces sp.WM-23
- Purification and Characterization of Arctium lappa L. (Edible Burdock) Polyphenol Oxidase
- Enhancement of Activities of Cellulases under High Hydrostatic Pressure
- Isolation and Identification of a Pyrogallol Oxidase Producing Microorganism and the Enzyme Production
- Purification and Properties of Three Xylanases from Aspergillus aculeatus
- Isolation and Some Properties of a Novel Killer Toxin-like Protein Produced by Streptomyces sp. F-287
- Isolation and Some Properties of Sorbitol Oxidase from Streptomyces sp. H-7775
- Identification of Carboxyl Residues in Pepstatin-Insensitive Carboxyl Proteinase from Pseudomonas sp. 101 that Participate in Catalysis and Substrate Binding
- A Novel Metalloproteinase, Almelysin, from a Marine Bacterium, Alteromonas sp. No. 3696 : Purification and Characterization
- Substrate Specificity of a Novel Alcohol Resistant Metalloproteinase, Vimelysin, from Vibrio sp. T1800
- Cloining and Expression of an Isovaleryl Pepstatin-Insensitive Carboxyl Proteinase Gene from Xanthomonas sp. T-22
- A Novel Proteinaceous Kex 2 Proteinase Inhibitor, Kexstatin, from Streptomyces platensis Q268
- A Novel Alcohol Resistant Metalloproteinase, Vimelysin, from Vibrio sp. T1800: Purification and Characterization
- Isolation and Characterization of Chitinase from a Flake-chitin Degrading Marine Bacterium, Aeromonas hydrophila H-2330
- Oxidative Decarboxylations of 4-Hydroxymandelic Acid and 2-(4-Hydroxyphenyl)glycine by Laccase from Coriolus versicolor and Bilirubin Oxidases from Trachyderma tsunodae and Myrothecium verrucaria
- Transformation of 7-(4-Hydroxyphenylacetamido)cephalosporanic Acid into a New Cephalosporin Antibiotic, 7-[1-Oxaspiro(2.5)octa-6-oxo-4, 7-diene-2-carboxamido]ceph-alosporanic Acid, by Laccase
- Coriolus versicolor Laccase Catalyzes the Decarboxylation of 2-(4-Hydroxyphenyl)-glycine and 4-Hydroxymandelic Acid
- Isolation and Characterization of a Proteinaceous Protease Inhibitor from Bacillus subtilis(Microbiology & Fermentation Industry)
- Molecular Cloning and Expression of a Gene Encoding a Novel Sorbitol Oxidase from Streptomyces sp.H-7775
- Additional Evidence for the Identity of Scytalidium lignicolum Acid Proteinases with the Carboxyl Proteinase Group : The Interaction between Angiotensin I and S-PI-Insensitive Acid Proteinases by Means of A Zinc(II)-Dye Complex as a Probe(Biological Chemi
- Characterization of the Complex between α_2-Macroglobulin and a Serine Proteinase from Bacillus natto(Biological Chemistry)
- Comparative Study on the Specificities of Several Fungal Aspartic and Acidic Proteinases towards the Tetradecapeptide of a Renin Substrate(Biological Chemistry)
- Carboxyl Proteinase Essential for Maturation of Basidiospores of Lentinus edodes (shiitake)
- Purification and Characterization of Glutamate Decarboxylase from Lactobacillus brevis IFO 12005
- Purification and Characterization of a Novel Type of Chitinase from Vibrio alginolyticus TK-22
- Purification and Characterization of an Alkaline Proteinase Produced by Pimelobacter sp. Z-483
- Isolation of Propioxatin A from Actinosynnema sp. SI-23 during a Screening for Serratia piscatorum Metalloproteinase Inhibitors
- Substrate Specificities of Pepstatin-Insensitive Carboxyl Proteinases from Gram-Negative Bacteria
- Purification and Properties of an S-PI(Pepstatin Ac)-insensitive Carboxyl Proteinase from a Xanthomonas sp. Bacterium(Biological Chemistry)
- Molecular Characterization of a Rhodotorula-lytic Enzyme from Paecilomyces lilacinus Having β-1, 3-Mannanase Activity
- A Halophilic Serine Proteinase from Halobacillus sp. SR5-3 Isolated from Fish Sauce : Purification and Characterization
- Purification and Characterization of Serine Proteinase from a Halophilic Bacterium, Filobacillus sp. RF2-5
- Interaction of Proteinaceous Protease Inhibitor of Bacillus subtilis with Intracellular Proteases from the Same Strain(Microbiology & Fermentation Industry)
- Novel β-D-Galactofuranose-containing High-mannose Type Oligosaccharides in Ascorbate Oxidase from Acremonium sp. HI-25
- Properties of Ascorbate Oxidase Produced by Acremonium sp. HI-25
- Isolation and Purification of Ascorbate Oxidase from Acremonium sp.HI-25
- An Acidic Ascorbate Oxidase Produced by Acremonium sp. HI-25(Biological Chemistry)
- Resynthesis of Reactive Site Peptide Bond and Temporary Inhibition of Streptomyces Metalloproteinase Inhibitor
- Identification of Reactive Site of a Proteinaceous Metalloproteinase Inhibitor from Streptomyces nigrescens TK-23
- Glutamate Decarboxylase from Lactobacillus brevis: Activation by Ammonium Sulfate
- Structural Evidence That Scytalidolisin (Formerly Scytalidopepsin A) Is a Serine-Carboxyl Peptidase of the Sedolisin Family
- Purification and Characterization of Cysteine Proteinase from a Baculovirus Gene
- Mutational Analysis of the Reactive Site Loop of Streptomyces Metalloproteinase Inhibitor, SMPI
- Different Action by Two Avicelases from Aspergillus aculeatus(Biological Chemistry)
- Reduced Cellulose as a Substrate of Cellulases(Biological Chemistry)
- Enzymatic Properties of Hydrocellulase from Aspergillus aculeatus
- A Novel Thermostable, S-PI(Pepstatin Ac)-insensitive Acid Proteinase from Thermophilic Bacillus novosp. Strain MN-32(Biological Chemistry)
- The Primary Structure of Pepstatin-Insensitive Carboxyl Proteinase Produced by Pseudomonas sp. No. 101
- Secretive Expression of the Aspergillus aculeatus Cellulase (FI-CMCase) by Saccharomyces cerevisiae
- Expression of the Cellulase (FI-CMCase) Gene of Aspergillus aculeatus in Saccharomyces cerevisiae
- Expression of the Cellulase (FI-CMCase) Gene of Aspergillus aculeatus in Escherichia coli
- Effects of α- and β-Arbutin on Activity of Tyrosinases from Mushroom and Mouse Melanoma
- Colorimetric Measurement of Total Activity of Leucine Aminopeptidase and Arylamidase in Serum Using Laccase-Catalyzed Oxidative Decarboxylation
- A New Microorganism Producing a Glucosyl Transfer Enzyme to Polyphenols
- Purification and Some Properties of a Thermostable Metal Proteinase Produced by Thermomicrobium sp. KN-22 Strain(Biological Chemistry)
- A Novel B-C-N Compound Derived from Melamine Diborate
- New Proteinacens α-Amylase inhibitor (T-76) from Streptomyces nitrosporeus
- Studies on β-Glucosidases from Soybeans That Hydrolyze Daidzin and Genistin : Isolation and Characterization of an Isozyme
- Subsite Prefernces of Pepstatin-Insensitive Carboxyl Proteinases from Bacteria
- Purification and Characterization of Alkaline Serine Proteinase from Photosynthetic Bacterium, Rubrivivax gelatinosus KDDS1
- An Alkaline Serine-Proteinase from a Bacterium Isolated from Bat Feces : Purification and Characterization
- Glutamate Decarboxylase from Lactobacillus brevis : Activation by Ammonium Sulfate