Cochinin B, a Novel Ribosome-Inactivating Protein from the Seeds of Momordica cochinchinensis(Biochemistry)
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概要
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Cochinin B, a novel ribosome-inactivating protein (RIP) with a molecular weight of 28kDa, was purified from the seeds of Momordica cochinchinensis (Cucurbitaceae). The isolation procedure entailed ammonium sulfate precipitation, cation-exchange chromatography on SP Sepharose column and size-exclusion chromatography on Superdex 75 column with a fast protein liquid chromatography (FPLC) system. The first twenty N-terminal amino acid residues of Cochinin B showed homology to type I RIPs from other Momordica species. The purified Cochinin B displayed a strong inhibitory activity on protein synthesis in the cell-free rabbit reticulocyte lysate system with IC_<50> of 0.36nM. Furthermore, it exhibited N-glycosidase activity and cytotoxicity against Vero cell line with IC_<50> higher than 1540 nM. Interestingly, Cochinin B manifested strong anti-tumor activities on human cervical epithelial carcinoma (HeLa), human embryonic kidney (HEK293) and human small cell lung cancer (NCI-H187) cell lines with IC_<50> of 16.9, 114 and 574 nM, respectively.
- 2007-03-01
著者
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ODA Kohei
Department of Applied Biology, Kyoto Institute of Technology
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SAIKI Ikuo
Division of Pathogenic Biochemistry, Institute of Natural Medicine, University of Toyama
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Leelamanit Wichet
Department of Biochemistry, Faculty of Pharmacy, Mahidol University
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Sakurai Hiroaki
Division of Pathogenic Biochemistry, Institute of Natural Medicine, University of Toyama
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Leelamanit Wichet
Department Of Biochemistry Faculty Of Pharmacy. Mahidol University
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Oda Kohei
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Saiki Ikuo
Division Of Pathogenic Biochemistry Department Of Bioscience Institute Of Natural Medicine Universit
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Sakurai Hiroaki
Division Of Pathogenic Biochemistry Institute Of Natural Medicine University Of Toyama
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CHUETHONG Juthamas
Department of Biochemistry, Faculty of Pharmacy. Mahidol University
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Saiki Ikuo
Division Of Pathogenic Biochemistry. Institute Of Natural Medicine University Of Toyama
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Sakurai Hiroaki
Division Of Pathogenic Biochemistry. Institute Of Natural Medicine University Of Toyama
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Chuethong Juthamas
Department Of Biochemistry Faculty Of Pharmacy. Mahidol University
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