A Novel Alcohol Resistant Metalloproteinase, Vimelysin, from Vibrio sp. T1800: Purification and Characterization

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概要

• 論文の詳細を見る

• We found a novel metalloproteinase, which has high activity at low temperatures and in the presence of organic solvents, in the culture supernatant of a marine bacterium, Vibrio sp. T1800. The metalloproteinase, named vimelysin, was purified from the dulture supernatant by three column chromatographies. About 150mg of purified vimelysin was obtained from 3.3 liters of the culture supernatant with a high yield of 57%. The purified vimelysin showed a single protein band on SDS-PAGE with molecular weight of 38,000. The isoelectric point of vimelysin was 4.3 by isoelectric f6cusing. The optimum pH of vimelysin was pH 8.0 or pH 6.5 using casein or furylacryloyl-glycyl-leucine amide (FAGLA) as substrates, respectively. The optimum temperature of vimelysin was 50℃ when cdsein was uSed as a substrate, but it was 15℃ when FAGLA was used as a substrate. Interestingly, vimelysin activity was completely retained after 48h of incubation at 25℃ in the presence of 50% ethanol. Moreover, vimelysin showed 40% activity of the control even in the presence of 10% ethanol, while thermolysin showed only 5% activity under the same conditions.

• 社団法人日本農芸化学会の論文
• 1996-03-23

著者

• ODA Kohei

  Department of Applied Biology, Kyoto Institute of Technology

• Sato R

  Graduate School Of Bio-application And Systems Engineering Tokyo University Of Agriculture And Techn

• Sato R

  Univ. Tokyo Tokyo Jpn

• Takahashi S

  Akita Res. Inst. Food And Brewing Akita

• TAKAHASHI Saori

  Department of Bioengineering, Akita Research Institute of Food and Brewing (ARIF)

• Sato Ryoichi

  Central Research Institute, Maruha, Tsukuba

• Oda Kohei

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• OKAYAMA Kiyoaki

  Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology

• OKUTOMI Keiichi

  Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology

• SHIMADA Masahiko

  Central Research Institute, Maruha

• Okutomi Keiichi

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• Oda K

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• Shimada M

  Univ. Electro‐communications Tokyo

• Suzuki Takahito

  Biological Laboratory Faculty Of Science Nara Women's University

• Sato Ryoichi

  Central Research Institute Maruha Co.

• Takahashi Saori

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• Shimada Masahiko

  Central Research Institute Maruha

• Sato Ryoichi

  Central Research Institute Maruha

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