Stimulative Effects of (22E,24R)-Ergosta-7,22-diene-3β,5α,6β-triol from Fruiting Bodies of Tricholoma auratum, on a Mouse Osteoblastic Cell Line, MC3T3-E1

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概要

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• We screened the differentiation-inducing activities of 39 mushroom extracts from Akita prefecture, Japan, on the mouse osteoblastic cell line, MC3T3-E1. Sixteen phosphate buffered saline (PBS), 8 boiled PBS, 14 ethanol and 12 methanol extracts induced alkaline phosphatase (ALP) activities, an indicator of MC3T3-E1 cell differentiation. The enzyme activities were markedly induced by extracts of Tricholoma auratum, and we isolated the active compound from methanol extracts of this mushroom. Physical data for the isolated active compound were identical to those for (22E,24R)-ergosta-7,22-diene-3β,5α,6β-triol (1). 1 induced ALP activities of MC3T3-E1 cells and promoted cell proliferation. To investigate the relationships between the chemical structure and differentiation-inducing activity of the compound, ALP-inducing activities of MC3T3-E1 cells by 1, ergosterol (2), ergocalciferol (3), cholesta-3β,5α,6β-triol (4), 7-dehydrocholesterol (5) and cholecalciferol (6) were tested. The enzyme activities of MC3T3-E1 cells were increased 3.0-fold by 10μM 1 and 2.4-fold by 10μM 4. However, 2, 3, 5 and 6 did not induce MC3T3-E1 cell ALP activity at 0.1-10 μM. These results suggested that the OH groups at C-5 and/or C-6 of 1 and 4 played an important role in their differentiation-inducing activities on MC3T3-E1 cells. Furthermore, 1 suppressed induction of MC3T3-E1 cell apoptosis by serum starvation.

• 公益社団法人日本薬学会の論文
• 2002-08-01

著者

• HATA Keishi

  Akita Research Institute for Food and Brewing (ARIF)

• Hata Keishi

  Akita Res. Inst. For Food And Brewing (arif)

• Hiwatashi Kazuyuki

  秋田県農林水産技術センター総合食品研究所

• Hiwatashi Kazuyuki

  Akita Res. Inst. Of Food And Brewing

• TAKAHASHI Saori

  Akita Research Institute of Food and Brewing

• Hirano Kaoru

  Faculty Of Pharmaceutical Sciences Kobe Gakuin University

• Takahashi S

  Kyoto Univ. Kyoto Jpn

• HORI Kazuyuki

  Akita Research Institute of Food and Brewing

• Kojima Hiroaki

  R & D Company Kobayashi Pharmaceutical Co. Ltd.

• Takahashi S

  Akita Res. Inst. Of Food And Brewing

• Takahashi Saori

  Akita Res. Inst. Of Food And Brewing

• Takahashi S

  Akita Res. Inst. Food And Brewing Akita

• SUGAWARA Fuyuki

  Akita Prefecture Forest Technical Center

• Takahashi Shunji

  Institute Of Molecular And Cellular Biosciences University Of Tokyo

• Takahashi Shunji

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• OHISA Naganori

  Akita Research Institute of Food & Brewing (ARIF)

• Hori K

  Kao Co. Ltd. Tochigi Jpn

• Hori K

  Akita Res. Inst. Of Food And Brewing

• Hori Kazuyuki

  Akita Research Institute Of Food & Brewing (arif)

• Hori Kazuyuki

  The Central Research Laboratory Hitachi Ltd.

• Hata Keishi

  Akita Res. Inst. Of Food And Brewing

• Kojima Hiroaki

  R&d Co. Kobayashi Pharmaceutical Co. Ltd.

• Hata K

  Akita Research Institute Of Food And Brewing

• Suzuki Takahito

  Biological Laboratory Faculty Of Science Nara Women's University

• Kojima Hiroaki

  Kobayashi Pharmaceutical Co. Ltd. R&d Company

• Hori Kazuyuki

  Akita Research Institute for Food and Brewing

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