Isolation of Human Renin Inhibitor from Soybean: Soyasaponin I Is the Novel Human Renin Inhibitor in Soybean

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概要

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• We found human renin inhibitory activity in soybean and isolated the active compound, soybean renin inhibitor (SRI). The physico-chemical data on the isolated SRI were identical with those of soyasaponin I. SRI showed significant inhibition against recombinant human renin, with an IC50 value of 30 μg/ml. Kinetic studies with SRI indicated partial noncompetitive inhibition, with a Ki value of 37.5 μM. On the other hand, SRI weakly inhibited pepsin, papain, and bromeline activities, but did not inhibit other proteinases, such as trypsin, kallikrein, angiotensin converting enzyme, and aminopeptidase M. Moreover, a significant (p<0.05) decrease in the systolic blood pressure of spontaneously hypertensive rats was observed when partially purified SRI was orally administrated at 40 mg/kg/d for 7 weeks. This is the first demonstration of a renin inhibitor from soybean, soyasaponin I.

• 社団法人 日本農芸化学会の論文

著者

• HORI Kazuyuki

  Institute for Food & Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Cente

• Gotoh Takeshi

  Department of Engineering in Applied Chemistry, Akita University

• Takahashi Saori

  Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Cen

• Takahashi S

  Akita Res. Inst. Of Food And Brewing

• Takahashi Shunji

  Institute Of Molecular And Cellular Biosciences University Of Tokyo

• SHINBO Mamoru

  Yamada Foods Co., Ltd.

• HIWATASHI Kazuyuki

  Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Cen

• YAMADA Seihan

  Yamada Foods Co., Ltd.

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