Immobilization of Biological Membranes by Sonication and Freeze-Thawing

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概要

• 論文の詳細を見る

• A rapid and convenient means of immobilizing biological membranes is described. Sonication for formation of small vesicles, diffusion of the vesicles in support beads, and freeze-thawing is carried out in an immobilized multi-enzyme system. Large amounts of brush border membranes from bovine kidney were immobilized in Sepharose CL-6B, Sephacryl S-500, and Sephacryl S-1000 and increased in that order. For the latter two beads, the amounts were further increased with repetition of freeze-thawing up to a maximum of three times. Particle distribution analysis reveals that small vesicles are enlarged by the freeze-thawing repetition, suggesting an immobilization mode in which enlarged vesicles are physically entrapped in the beads. The bead-immobilized membrane vesicles were relatively stable, exhibiting constant enzyme activities for at least 6 h under packed column operation.

• 社団法人 化学工学会の論文

著者

• Gotoh Takeshi

  Department of Engineering in Applied Chemistry, Akita University

• Kikuchi Ken-Ichi

  Department of Engineering in Applied Chemistry, Akita University

• Kikuchi Ken-ichi

  Department Of Engineering In Applied Chemistry Akita University

• Gotoh Takeshi

  Department Of Engineering In Applied Chemistry Akita University

• Gotoh Takeshi

  Department Of Materials-process Engineering & Applied Chemistry For Environments Faculty Of Engi

• Kikuchi K

  Akita Univ. Akita Jpn

• Gotoh Takeshi

  Department Of Engineering And Applied Chemistry Graduate School Of Engineering And Resource Science

• KIKUCHI KEN-ICHI

  Department of Chemical Engineering for Resources, Akita University

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