Refolding and Activation of Human Prorenin Expressed in Escherichia coli : Application of Recombinant Human Renin for Inhibitor Screening

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概要

• 論文の詳細を見る

• Human prorenin was expressed in Escherichia coli as a fusion protein of thioredoxin. The chimeric protein, which accumulated insoluble inclusion bodies, was solubilized in 4 M guanidine–HCl and refolded by an arginine-detergent buffer system and by systematic dialysis. The refolded fusion prorenin was activated by trypsin. The antiserum against human kidney renin specifically inhibited the recombinant human renin activity. Using the recombinant human renin, we screened its inhibitory activity in fermented soybean paste (miso) and demonstrated that miso contained renin inhibitory activity derived from soybean. The IC50 values for soybean and steamed soybean extracts were determined to be 1.9 and 1.6 mg/ml, respectively. This is the first demonstration of renin inhibitory activity in miso and soybean.

• 社団法人 日本農芸化学会の論文
• 2006-12-23

著者

• INOUE HIROYASU

  Department of Pediatrics, Tokai University School of Medicine

• Hiwatashi Kazuyuki

  秋田県農林水産技術センター総合食品研究所

• Hiwatashi Kazuyuki

  Akita Res. Inst. Of Food And Brewing

• Hiwatashi Kazuyuki

  東北大学 農学研究科栄養学

• Takahashi S

  Akita Res. Inst. Food And Brewing Akita Jpn

• TAKAHASHI Saori

  Akita Research Institute of Food and Brewing

• Takahashi S

  Kyoto Univ. Kyoto Jpn

• HORI Kazuyuki

  Akita Research Institute of Food and Brewing

• Inoue Hiroyasu

  Department Of Food Science And Nutrition Nara Women's University

• Takahashi S

  Department Of Biosciences Teikyo University

• Kojima Hiroaki

  R & D Company Kobayashi Pharmaceutical Co. Ltd.

• Takahashi S

  Akita Res. Inst. Of Food And Brewing

• Takahashi Saori

  Akita Res. Inst. Of Food And Brewing

• Takahashi S

  Akita Res. Inst. Food And Brewing Akita

• Takahashi Senji

  Department Of Biosciences Teikyo University

• Takahashi Shunji

  Institute Of Molecular And Cellular Biosciences University Of Tokyo

• Takahashi Shunji

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Inoue Hiroyasu

  Department Of Applied Chemistry Faculty Of Technology Kanagawa University

• Inoue Hiroyasu

  Department Of Food Science And Nutrition Faculty Of Human Life And Environment Nara Women's Uni

• Takahashi S

  秋田県農林水産技術センター総合食品研究所

• OGASAWARA Hironobu

  Akita Research Institute for Food and Brewing

• WATANABE Takayuki

  Akita Research Institute for Food and Brewing

• KUMAGAI Masanori

  Akita Research Institute for Food and Brewing

• Hori K

  Kao Co. Ltd. Tochigi Jpn

• Hori Kazuyuki

  Akita Research Institute Of Food & Brewing (arif)

• Hori Kazuyuki

  The Central Research Laboratory Hitachi Ltd.

• Kojima Hiroaki

  R&d Co. Kobayashi Pharmaceutical Co. Ltd.

• Suzuki Takahito

  Biological Laboratory Faculty Of Science Nara Women's University

• Takahashi S

  Department Of Biology The University Of Tokyo

• Takahashi Satoru

  Institute Of Basic Medical Sceinces And Center For Tara

• Hori Kazuyuki

  Akita Research Institute for Food and Brewing

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