Purification and Characterization of a Novel Prolyl Aminopeptidase from Maitake (Grifola frondosa)
スポンサーリンク
概要
- 論文の詳細を見る
We have found a novel prolyl aminopeptidase in Grifola frondosa. The enzyme was purified by DEAE-Sepharose CL-6B, Butyl-Toyopearl, Sephacryl S-100, and Mono-Q column chromatographies. The purified enzyme exists as a dimer and gives high activity toward L-proline-p-nitroanilide. The enzyme was strongly inhibited by p-chloromercuribenzoic acid and iodoacetic acid and markedly inhibited by phenylmethylsulfonyl fluoride and arphamenin A.
- 社団法人 日本農芸化学会の論文
- 2004-06-23
著者
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Kagaya Akira
Akita Jujo Chemicals Co Ltd
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Kagaya Akira
Akita Jujo Chemicals Co. Ltd.
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Hiwatashi Kazuyuki
秋田県農林水産技術センター総合食品研究所
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Hiwatashi Kazuyuki
東北大学 農学研究科栄養学
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SUGIYAMA Toshihiro
Department of Biochemistry, Akita University School of Medicine
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Takahashi S
Akita Res. Inst. Food And Brewing Akita Jpn
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Takahashi S
Kyoto Univ. Kyoto Jpn
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Takahashi Kenji
Dep. Of Radiological Life Sciences Hirosaki Univ. Graduate School Of Health Sciences
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Takahashi K
School Of Life Sciences Tokyo Univ. Of Pharmacy And Life Sciences
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Takahashi Kayoko
Central Research Lnstitute Meiji Milk Products Co. Ltd.
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Takahashi S
Department Of Biosciences Teikyo University
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Takahashi S
Akita Res. Inst. Of Food And Brewing
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Takahashi Saori
Akita Res. Inst. Of Food And Brewing
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Takahashi S
Akita Res. Inst. Food And Brewing Akita
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Takahashi K
Tokyo Univ. Agriculture And Technol. Tokyo Jpn
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TAKAHASHI Keitaro
Department of Physics, School of Science, University of Tokyo
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Takahashi Senji
Department Of Biosciences Teikyo University
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Takahashi Shunji
Institute Of Molecular And Cellular Biosciences University Of Tokyo
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Takahashi Shunji
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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HIWATASHI Kazuyuki
Department of Bioengineering, Akita Research Institute of Food and Brewing (ARIF)
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HORI Kazuyuki
Department of Bioengineering, Akita Research Institute of Food and Brewing (ARIF)
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INOUE Shunzo
Akita Jujo Chemicals Co., Ltd.
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TAKAHASHI Saori
Department of Bioengineering, Akita Research Institute of Food and Brewing (ARIF)
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Inoue Shunzo
Akita Jujo Chemicals Co. Ltd.
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Hori K
Kao Co. Ltd. Tochigi Jpn
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Hori K
Akita Res. Inst. Of Food And Brewing
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Hori Kazuyuki
The Central Research Laboratory Hitachi Ltd.
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Takahashi Ken-ichi
Department Of Industrial Chemistry Faculty Of Engineering Chiba Institute Of Technology
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Kojima Hiroaki
R&d Co. Kobayashi Pharmaceutical Co. Ltd.
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Takahashi Kyoko
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
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Kageyama T
Kyoto Univ. Aichi Jpn
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Takahashi Keitaro
Department Of Orthodontics School Of Dentistry At Tokyo Nippon Dental University
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Suzuki Takahito
Biological Laboratory Faculty Of Science Nara Women's University
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Sugiyama Toshihiro
Department Of Biochemistry School Of Medicine Akita University
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Sugiyama Toshihiro
Department Of Biochemistry Akita University School Of Medicine
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Takahashi Saori
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Hori Kazuyuki
Department Of Bioengineering Akita Research Institute Of Food And Brewing
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Sugiyama Toshihiro
Department Of Biochemistry Akita University Graduate School Of Medicine
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Takahashi Kenji
School Of Life Sciences Tokyo University Of Pharmacy And Life Sciences
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Takahashi Kenji
Laboratory Of Molecular Biochemistry School Of Life Science Tokyo University Of Pharmacy And Life Sc
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Takahashi Satoru
Institute Of Basic Medical Sceinces And Center For Tara
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