Quantitative Detection of Apoptotic Thymocytes in Low-dose X-irradiated Mice by an Anti-single-stranded DNA Antibody

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概要

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• The quantitative detection of apoptotic cells in low frequency in the thymus of mice irradiated with X-rays using an anti-single-stranded DNA antibody was explored. The antibody against single-stranded DNA (anti-ssDNA) was obtained with rabbits hyperimmunized with complexes of alkaline-denatured calf thymus DNA. AKR female mice were irradiated with 10 to 100 cGy or 4 Gy X-rays; thereafter, thymus sections were prepared at various times after irradiation. The detection and counting of apoptotic cells in the section were performed after histochemical staining using an anti-ssDNA antibody. The results demonstrate that, although sensitive and quantitative detection of apoptotic cells in irradiated thymus using the anti-ssDNA antibody is possible, the sensitivity is lower compared to that of in situ endlabeling methods, such as TUNEL or ISEL. The antibodies could also be used for rat thymus and spleen. In addition, an increase in positively stained cells by both methods was detected as early as 6 min after the irradiation of mice.

• 日本放射線影響学会の論文

著者

• YAMADA Takeshi

  Department of Cardiology, Kouseikai Takeda Hospital

• KAWARADA Yoshihiko

  Department of Biochemistry, Akita University School of Medicine

• SUGIYAMA Toshihiro

  Department of Biochemistry, Akita University School of Medicine

• Terada Kunihiko

  Department of Respiratory Medicine, Tenri Hospital

• Ohyama H

  Research Center For Radiation Safety Natl. Inst. Radiol. Sci.

• Ohyama Harumi

  放射線医学総合研究所

• Ohyama Harumi

  National Institute Of Radiological Sciences Radiation Hazards Research Group

• Ohyama Harumi

  Division Of Radiobiology And Biodosimetry National Institute Of Radiological Sciences

• Ohyama Harumi

  Divs. Biol. And Radiation Health National Institute Of Radiological Sciences

• Yamamoto Koji

  Department Of Medical Biochemistry Faculty Of Medical And Pharmaceutical Science Kumamoto University

• Yamagata K

  Department Of Metabolic Medicine Graduate School Of Medicine Osaka University

• OHYAMA Harumi

  NIRS.

• Terada Kunihiko

  Department Of Biochemistry Akita University School Of Medicine

• Fujita Kazuko

  Radiat. Hazards Res. Group Nirs

• Fujita Kazuko

  電力中央研究所

• Fujita Kazuko

  Department Of Biology Toho University School Of Medicine:division Of Biomolecular Science Graduate S

• Fujita Kazue

  Low Dose Radiat. Res. Center Central Res. Inst. Electric Power Industry

• Yamada T

  National Cancer Center Res. Inst. Tokyo Jpn

• Kawarada Yoshihiko

  Kurume Univ. Fukuoka Jpn

• Fujita Kazuko

  Toho Univ. School Of Med. Dept. Biol.

• Fujita Kazuko

  Toho Univ. Graduate Sch. Of Biomolecular Sci .

• Sugiyama Toshihiro

  Department Of Biochemistry School Of Medicine Akita University

• Sugiyama Toshihiro

  Department Of Biochemistry Akita University School Of Medicine

• Kawarada Yoshihiko

  Department Of Biochemistry Akita University School Of Medicine

• Sugiyama Toshihiro

  Department Of Biochemistry Akita University Graduate School Of Medicine

• Yamada Takeshi

  Toho Univ. Sch. Med

• Yamada Takeshi

  Department Of Anesthesiology Iida Municipal Hospital

• Yamada Takeshi

  Toho Univ. School Of Med. Dept. Biol.

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