Fas-independent Apoptosis Induced by UVC in p53-Mutated Human Epithelial Tumor A431 Cells through Activation of Caspase-8 and JNK/SAPK
スポンサーリンク
概要
- 論文の詳細を見る
We previously observed that p53-mutated human epithelial tumor A431 cells underwent apoptosis after ultraviolet C (UVC) irradiation through the caspases-8 and -3 pathway. Fas/FasL is known to initiate apoptosis in several cell lines via caspase-8 activation. Then, to determine if Fas/FasL mediates apoptosis in A431, we investigated Fas expression and modulation in UVC-irradiated A431 cells. A431 constitutively expressed Fas, which gradually decreased after UVC-irradiation. Pretreatment with a neutralizing anti-Fas antibody, ZB4, did not abrogate the UVC-induced apoptosis. An agonistic anti-Fas antibody, CH11, very slowly induced apoptosis in A431, suggesting that the constitutively expressed Fas had a low functional potential. Hence, UVC-induced apoptosis in A431 seems to occur independent of the Fas signal. Interestingly, however, a pretreatment with CH11 remarkably potentiated UVC-induced apoptosis. An inhibitor of caspase-8, Ac-IETD-CHO, partially inhibited UVC-induced apoptosis. JNK was phosphorylated immediately after exposure to UVC, prior to apoptotic chromatin condensation. Our data suggest that the activation of caspase-8 occurs independent of Fas upregulation, and that JNK/ SAPK contributes to UVC-induced apoptosis in human epithelial A431 cells.
- 日本放射線影響学会の論文
著者
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WANG Bing
Radiation Effect Mechanisms Research Group, National Institute of Radiological Sciences
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Wang Bing
National Institute of Radiological Sciences
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Choi Keun
Radiation Hazards Research Group National Institute Of Radiological Sciences
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Wang Bing
Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences
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Hama‐inaba H
Research Center For Radiation Safety Natl. Inst. Radiol. Sci.
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Hama-inaba Hiroko
放射線医学総合研究所
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Yamada Takeshi
Radiat. Hazards Res. G. Nirs
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Ohyama H
Research Center For Radiation Safety Natl. Inst. Radiol. Sci.
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Ohyama Harumi
放射線医学総合研究所
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Ohyama Harumi
National Institute Of Radiological Sciences Radiation Hazards Research Group
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Ohyama Harumi
Division Of Radiobiology And Biodosimetry National Institute Of Radiological Sciences
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Ohyama Harumi
Divs. Biol. And Radiation Health National Institute Of Radiological Sciences
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Wang B
Research Center For Radiation Safety Natl. Inst. Radiol. Sci.
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OHYAMA Harumi
NIRS.
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HAYATA Isamu
Radiation Hazards Research Groupe, Natl. Inst. Radiat, Sci.
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OHYAMA Harumi
Radiat. Hazards Res. Group, NIRS
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Hayata I
Natl. Inst. Radiol. Sci.:cent. Res. Inst. Elec. Pow. Ind.
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Hayata Isamu
Radiat. Hazards Res. Gr. Natl. Inst. Radiol. Sci
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Hayata Isamu
Div. Radiobiol. Biodosim.
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Hayata Isamu
Radiation Hazards Research Group Nirs
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Yamada T
National Cancer Center Res. Inst. Tokyo Jpn
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HAMA-INABA HIROKO
Radiation Hazards Research Group, National Institute of Radiological Sciences
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HAGINOYA KEIKO
Radiation Hazards Research Group, National Institute of Radiological Sciences
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Haginoya Keiko
Radiation Hazards Research Group National Institute Of Radiological Sciences
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Hayata I
National Inst. Radiological Sci. Chiba Jpn
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Hayata Isamu
Radiation Hazards Research Groupe Natl. Inst. Radiat Sci.
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Wang Bing
Univ. Tokyo School Of Sci. Zoological Institute
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Hayata Isamu
Radiation Hazards Research Group
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Hayata Isamu
Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences
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Yamada Takeshi
Toho Univ. Sch. Med
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Yamada Takeshi
Toho Univ. School Of Med. Dept. Biol.
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