UVC-induced Apoptosis in Human Epithelial Tumor A431 Cells : Sequence of Apoptotic Changes and Involvement of Caspase (-8 and -3) Cascade
スポンサーリンク
概要
- 論文の詳細を見る
Human epidermoid tumor A431 cells underwent apoptosis following exposure to ultraviolet C (UVC). The apoptosis was of the interphase death type, and mostly occurred within one cell cycle, independent of the cell-cycle phases. We further examined the detailed sequential order of apoptotic changes in cells after UVC exposure and the involvement of caspases using six caspase inhibitors. The loss of mitochondrial transmembrane potential (ΔΨm) appeared in the earliest phase; subsequently, the chromatin condensation and DNA-fragmentation occurred. Cell shrinkage and loss of the plasma-membrane integrity, judged by propidium iodide (PI) staining, were observed in the later phase. A broad-spectrum caspase inhibitor, Z-VAD-fmk, completely prevented all apoptotic changes, except for the depletion of ΔΨm. Both Ac-DEVD-CHO and Ac-IETD-CHO, inhibitors of caspase -3 and -8, respectively, effectively inhibited typical chromatin condensation to almost the same extent. However, the nuclei still showed partial condensation. A caspase -9 inhibitor, Ac-LEHD-CHO, did not prevent chromatin condensation, though it partially inhibited cell-size reduction and PI-stainability. None of the caspase inhibitors could inhibit the ΔΨm reduction. These results strongly suggest that the collapse of ΔΨm is not a part of the central apoptotic machinery, and that caspase cascade(s), especially caspase -8 to -3, play an important role in UVC-induced apoptosis in A431.
- 日本放射線影響学会の論文
著者
-
Yamada Takeshi
Division of cardiology, Department of Medicine, Nihon University School of Medicine
-
Wang Bing
National Institute of Radiological Sciences
-
Choi Keun
Radiation Hazards Research Group National Institute Of Radiological Sciences
-
Wang Bing
Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences
-
Hama‐inaba H
Research Center For Radiation Safety Natl. Inst. Radiol. Sci.
-
Hama-inaba Hiroko
放射線医学総合研究所
-
Hama-inaba Hiroko
Division Of Genetics National Institute Of Radiological Sciences
-
Ohyama H
Research Center For Radiation Safety Natl. Inst. Radiol. Sci.
-
Ohyama Harumi
放射線医学総合研究所
-
Ohyama Harumi
National Institute Of Radiological Sciences Radiation Hazards Research Group
-
Ohyama Harumi
Division Of Radiobiology And Biodosimetry National Institute Of Radiological Sciences
-
Ohyama Harumi
Divs. Biol. And Radiation Health National Institute Of Radiological Sciences
-
Wang B
Research Center For Radiation Safety Natl. Inst. Radiol. Sci.
-
OHYAMA Harumi
NIRS.
-
HAYATA ISAMU
Division of Radiobiology and Biodosimetry, National Institute of Radiological Sciences
-
Odaka Takeko
Division Of Radiobiology And Biodosimetry National Institute Of Radiological Sciences
-
Hayata I
Natl. Inst. Radiol. Sci.:cent. Res. Inst. Elec. Pow. Ind.
-
Hayata Isamu
Division Of Radiobiology And Biodosimetry National Institute Of Radiological Sciences
-
Yamada T
National Cancer Center Res. Inst. Tokyo Jpn
-
Yamada Takeshi
Division Of Cardiology Department Of Medicine Nihon University School Of Medicine
-
CHOI KEUN
Division of Radiobiology and Biodosimetry, National Institute of Radiological Sciences
-
HAGINOYA KEIKO
Division of Radiobiology and Biodosimetry, National Institute of Radiological Sciences
-
Haginoya Keiko
Radiation Hazards Research Group National Institute Of Radiological Sciences
-
Wang Bing
Univ. Tokyo School Of Sci. Zoological Institute
-
Yamada Takeshi
Division Of Biology National Institute Of Radiological Sciences
-
Yamada Takeshi
Toho Univ. Sch. Med
-
Yamada Takeshi
Toho Univ. School Of Med. Dept. Biol.
関連論文
- PJ-195 Effects of Amiodarone and Ibutilide on The Atrial Action Potential Duration and Conduction Time After Cardioversion of Chronic Atrial Fibrillation(Arrhythmia, Pharmacological Therapy 2 (A) : PJ33)(Poster Session (Japanese))
- OJ-223 Relation Between Transverse Conduction Capability and the Anatomy of the Crista Terminalis in Patients with and without Atrial Flutter(Arrhythmia, Diagnosis/Pathophysiology/EPS 8 (A) : OJ25)(Oral Presentation (Japanese))
- OJ-222 Increased Intraatrial Conduction Time in Patients with Brugada Syndrome Assessed By Signal Averaged P wave Duration(Arrhythmia, Diagnosis/Pathophysiology/EPS 8 (A) : OJ25)(Oral Presentation (Japanese))
- OJ-100 P-Wave Morphology of Arrhythmogenic Focus in Patients with Atrial Fibrillation Originating from Superior Vena Cava or Right Superior Pulmonary Vein(Arrhythmia, Diagnosis/Pathophysiology/EPS 7 (A) : OJ12)(Oral Presentation (Japanese))
- OJ-099 Full-Motion, Three Dimensional Pulmonary Vein Imaging Using Intracardiac Echocardiography After Isolation of Pulmonary Veins by Radiofrequency Ablation(Arrhythmia, Diagnosis/Pathophysiology/EPS 7 (A) : OJ12)(Oral Presentation (Japanese))
- PJ-417 Combined Use of Ensite Non-Contact Mapping System and Template Matching for Radiofrequency Catheter Ablation of Right Ventricular Outflow Tract Tachycardia(PJ070,Ventricular Arrhythmia (Clinical/Diagnosis/Treatment) 1 (A),Poster Session (Japanese),
- PJ-011 The Upper Turnover Site in the Reentry Circuit of Common Atrial Flutter-3 Dimensional Mapping and Entrainment Study(PJ002,Atrial/Supraventricular Arrhythmia (Clinical/Pathophysiology) (A),Poster Session (Japanese),The 73rd Annual Scientific Meeting
- PE-489 Idiopathic Ventricular Fibrillation Presenting with Prominent J Wave(PE082,Ventricular Arrhythmia (Clinical/Diagnosis/Treatment) (A),Poster Session (English),The 73rd Annual Scientific Meeting of the Japanese Circulation Society)
- Combined Effect of Pulmonary Vein Isolation and Ablation of Cardiac Autonomic Nerves for Atrial Fibrillation
- Complete AV Block Developing in a Patient With Manifest Ventricular Preexcitation
- OJ-101 Magnetic Resonance Imaging Detects Chronic Left Atrial Wall Injury Post Ablation of Atrial Fibrillation(OJ17,Atrial/Supraventricular Arrhythmia (Clinical/Treatment) (A),Oral Presentation (Japanese),The 73rd Annual Scientific Meeting of The Japanese
- P Wave Morphology of an Arrhythmogenic Focus in Patients With Atrial Fibrillation Originating From a Pulmonary Vein or the Superior Vena Cava
- PJ-420 High Sensitivity C-Reactive Protein : A Novel Predictor for Recurrence of Atrial Fibrillation after Catheter Ablation of Persistent Atrial Fibrillation(Atherosclerosis, clinical-11, The 71st Annual Scientific Meeting of the Japanese Circulation Soc
- PJ-347 High Speed Rotational Coronary Venous Angiography Improves Delineation of Coronary Venous Anatomy during Cardiac Resynchronization Therapy(CT/DSA-08, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-606 lartogenic Postatrial Fibrillation Ablation Left Atrial Tachicardia/Fulutter(Arrhythmia, diagnosis/pathophysiology/EPS-13 (A) PJ102,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-459 Catheter Ablation of Permsnent Atrial fibrillaion(Arrhythmia, diagnosis/pathophysiology/EPS-12 (A) PJ77,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- Gene Silencing of Tead3 Abrogates Radiation-induced Adaptive Response in Cultured Mouse Limb Bud Cells
- Differences in Apoptosis between Two Mouse Strains Possessing Different Susceptibility to Radiation-Induced Thymoma
- Electrocardiographic and Electrophysiologic Characteristics in Patients With Brugada Type Electrocardiogram and Inducible Ventricular Fibrillation : Single Center Experience
- Electrophysiologic and Anatomical Characteristics of the Right Atrial Posterior Wall in Patients With and Without Atrial Flutter : Analysis by Intracardiac Echocardiography
- Identifying the Origin of Right and Left Ectopic Atrial Beats Triggering Atrial Fibrillation before Atrial Transseptal Procedure
- PJ-615 Prognostic Value of Induced Ventricular Fibrillation in Patients with Brugada Syndrome : Single Center Experience(Arrhythmia, diagnosis/pathophysiology/EPS-14 (A) PJ103,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the
- Microarray Analysis of Differentially Expressed Genes in the Kidneys and Testes of Mice after Long-term Irradiation with Low-dose-rate γ-rays
- Induction of Rhodanese, a Detoxification Enzyme, in Livers from Mice after Long-term Irradiation with Low-dose-rate Gamma-rays
- Atrial Electrophysiological Abnormality in Patients With Brugada Syndrome Assessed by P-Wave Signal-Averaged ECG and Programmed Atrial Stimulation
- PIK3CA mutation and amplification in human lung cancer
- Role of poly(ADP-ribose) polymerase in radiation-induced apoptosis
- Radiation hormesis : Stimulatory effects of low doses ionizing radiation
- Apoptosis in Murine Lymphoma Cells induced by Daunorubicin, a Chemotherapeutic Agent
- Structural Analysis of Chromosome Damage Induced with X-ray or Heavy Ion Radiation by Atomic ForceMicroscope
- Radiation-induced Apoptosis Responsible for Limb Teratogenesis in Embryonic Mice
- Radiation Induced Apoptosis in the Mouse Limb Bud Cells in vivo and in vitro
- 249 Prenatal Radiation-Induced Teratogenesis and Adaptive Response Modified by p53 and General Caspase Inhibitors(Apoptosis related, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
- Radiation-Induced Teratogenesis and Adaptive Response in the Late Period of Organogenesis in Mice : I. Dose Rate Effects
- Molecular Mechanisms Underlying Radiation-Induced Apoptosis in Radiosensitive Murine Thymoma Cell Line 3SB
- UV-lnduced Apoptosis in Thymic Lymphoma Cells : Cell Cycle uependency.
- Effects of Serine Protease Inhibitors on the Radiation-induced Apoptosis in Thymocytes and Lymphoma Cells.
- Effect of Smoking on Chromosomes Compared with That of Radiation in the Residents of a High-Background Radiation Area in China
- Imperceptible Effect of Radiation Based on Stable Type Chromosome Aberrations Accumulated in the Lymphocytes of Residents in the High Background Radiation Area in China
- O-06. Chromosome aberrations in the lymphocytes of residents in the high background radiation area in Guangdong province in China(Abstracts of the oral and poster presentations, Abstracts of the 54th Annual Meeting of the Society of Chromosome Research)
- Chromosome Translocation in Residents of the High Background Radiation Areas in Southern China
- Apoptosis in Mouse Spleen after Daily Low Dose Whole Body X-Irradiation
- Electron Microscopic Observation of Apoptosis and Necrosis in Murine Thymus after Whole-Body X-Irradiation
- Apoptosis in Murine Thymus and Spleen after Protracted Irradiation of Low Dose X-Rays
- Induction of Apoptosis in Mouse Thymus after Fractionated Exposure to Low Dose X-Rays
- Proteolytic cleavage of XRCC4 protein during apoptosis
- Cleavage of RAD51 protein during apotosis.
- Molecular Mechanisms Involved in Radiation-Induced Apoptosis and Necrosis In Rat Thymocytes
- Difference in mode of radiation-inducedapoptosis between L5178Y and its radiosensitive mutant L5178Y-S
- Robot System for Preparing Lymphocyte Chromosome
- Effects of Caspase Inhibitors on Nuclear Protein NP95 Depletion Associated with Radiation-induced Apoptosis in Murine ThymicLymphoma 3SB Cells.
- Involvement of Protein Kinase C in Radiation-induced Apoptosis Regulation in 3SBH5 Cells
- Low-Dose Irradiation Mediates Ca^ Signaling in Cultured Rat Hepatocytes
- Effect of Low Dose Radiation on Health Estimated by Cytogenetic Studies
- P-14. Aneuploidy caused by chromosomes showing premature centromere separation in a woman living in the high natural radiation area in Ramsar, Iran(Abstracts of the oral and poster presentations)
- Dose-effect Relationship of Dicentric and Ring Chromosomes in Lymphocytes of Individuals Living in the High Background Radiation Areas in China
- Rapid Decrease in the Expression of a Nuclear Protein NP95 Associated with Radiation-induced Apoptosis inMurine Thymic Lymphoma 3SB Cells.
- Involvement of Caspases in the Radiation-induced Apoptosis of Radiosensitive Murine Thymoma 3SB Cells
- Implication of Protein Kinase C Activation in the Radiation-Induced Apoptosis in Radiosensitive Murine Thymoma Cell 3SB
- Mechanisms for Radiation-induced Apoptosis in a Radiosensitive Murine Thymoma Cell Line 3SB
- Apoptosis in Radiosensitive Murine Lymphoma Cell Line 3SB and its Radioresistant Mutants
- Visualization of DNA repair protein-DNA interaction by Atomic Force Microscope
- Structural Analysis of Irradiated Plasmid DNA by Atomic Force Microscope
- 236 Distinct functions of protein kinase C subtypes in the regulation of radiation-induced apoptosis in murine thymic lymphoma cells(Apoptosis related, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
- Effect of 0.5 Gy Pre-expore on the PFC of Lethally Irradiated Mice
- Rescue of Lethally Irradiated Mice from Hematopoietic Death by Pre-expousure to 0.5 Gy X-rays withoutRecovery from Peripheral Blood Cell Depletion
- Fas-independent Apoptosis Induced by UVC in p53-Mutated Human Epithelial Tumor A431 Cells through Activation of Caspase-8 and JNK/SAPK
- Radioadaptation in Embryogenesis Is Related to p53 Gene Status
- Long Term Effects Observed in Mice Rescued by 0.5 Gy Pre-exposure from Lethal Irradiation
- UVC-induced Apoptosis in Human Epithelial Tumor A431 Cells : Sequence of Apoptotic Changes and Involvement of Caspase (-8 and -3) Cascade
- Radioadaptation in Embryogenesis during Late Period of Organogenesis and Postnatal Retardation in the Prenatally Primed Mice
- OK432-lnduced Modification of Radioadaptive Response in Mice
- Modification of Acquired Radio-Resistance in Mice by OK432
- OK432 Modification of Radio-Adaptive Response
- Induction of Radioresistance in Mice by Low Dose Pre-irradiation of X-Rays
- HIMAC Carbon-12 Induced Apoptosis in Murine Leukemia Cells, L5178Y and L5178YS
- Acquired Radioresistance in Hematopoiesis in C57BL Mice
- Production of Peroxides Associated with Radiation-induced Apoptosis in Rat Thymocytes.
- Analysis or Radioresistant Variants Derived from 3SB Cell
- Quantitative Detection of Apoptotic Thymocytes in Low-dose X-irradiated Mice by an Anti-single-stranded DNA Antibody
- C-3-44 Polarization dependency measurements on birefringence-reduced cascaded FBGs
- C-3-107 Birefringence reduction on cascaded fiber Bragg gratings
- PJ-194 Effect of Ibutilide on the Action Potential Duration Kinetics in Human Atrial Fibrillation(Arrhythmia, Pharmacological Therapy 2 (A) : PJ33)(Poster Session (Japanese))
- Acquired Radioresistance in Hematopolesis in Mice
- Radiation-induced Apoptosis in Murine Thymic Lymphoma : Cell Cycle Dependency
- Effects of UVC-Irradiation on Cultured Mouse Limb Bud Cells
- Modification of Radiation-Induced Apoptosis Affects the Severity of Limb Teratogenesis in Embryonic Mice
- Detection of Apoptotic cells in thymus of rat irmdiated with low dose
- Synergistic Effect by Heavy-Ion Beams and a Hypoxic Sensitizer
- EFFECT OF ORGANICALLY BOUND TRITIUM (OBT) ON PRE-IMPLANTATION MOUSE EMBRYOS INVITRO
- DNA Damage, Its Repair and UVC-Induced Apoptosis in Murine Thymoma Cells 3SB.
- Gene Silencing of Tead3 Abrogates Radiation-induced Adaptive Response in Cultured Mouse Limb Bud Cells
- Effects of Prenatal Exposure to Low-dose β Radiation from Tritiated Water on the Neurobehavior of Mice
- Transcription Factor-recognition Sequences Potentially Involved in Modulation of Gene Expression after Exposure to Low-dose-rate γ-rays in the Mouse Liver
- Cell Cycle Dependency of Apoptosis in Thymic Lymphoma Cells (3S11) Induced by Carbon Beam Irradiation
- Comparison of Two Types of Coated Slide Glass Using a New Autofocusing Method for Microscopic Image of Chromosomes
- DNA repair mechanisms of S. pombe III. Radiation-resistant gene redM12 encoding a novel ubiquitin-conjugating enzyme E2.
- Cloning of stress inducible genes of S. pombe.
- Effect of Estradiol on Radiation-induced Chromosome Aberrations in Human Lymphocytes
- Trp53 Activity Is Repressed in Radio-adapted Cultured Murine Limb Bud Cells