Robot System for Preparing Lymphocyte Chromosome

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概要

• 論文の詳細を見る

• Towards the automatization of the scoring of chromosome aberrations in radiation dosimetry with the emphasis on the improvement of biological preparations, the conventional culture and harvesting method was modified. Based on this modified method, a culture and harvest robotic system (CHROSY) for preparing lymphocyte chromosome was developed. The targeted points of the modification are as in the following. 1. Starting culture with purified lymphocytes in a fixed cell number. 2. Avoiding the loss of cells in changing the liquids following centrifugalization. 3. Keeping the quantity of the liquids to be applied to the treatments of cells fixed. 4. Building a system even a beginner can handle. System features are as follows. 1. Operation system: Handling robot having 5 degrees of freedom; a rotator incubator with an automatic sliding door; units for setting and removing pipette tips; a centrifuge equipped with a position adjuster and an automatic sliding door; two aluminum block baths; two nozzles as pipettes and aspirators connected to air pumps; a capping unit with a nozzle for CO_2 gas; a compressor; and an air manipulated syringe. 2. Control system: NEC PC-9801RX21 with CRT; and program written in Basic and Assembly languages on MS-DOS. It took this system 2 hours and 25 minutes to harvest 2 cultures. A fairly good chromosome slide was made from the sample harvested by CHROSY automatically.

• 日本放射線影響学会の論文

著者

• HAYATA Isamu

  National Institute of Radiological Sciences

• Okabe Nobuo

  Research Laboratory Nikon Corp.

• FURUKAWA Akira

  National Institute of Radiological Sciences

• Yamamoto M

  Hiroshima Univ. Hospital Hiroshima Jpn

• Hayata I

  Natl. Inst. Radiol. Sci.:cent. Res. Inst. Elec. Pow. Ind.

• Furukawa Akira

  Natl. Inst. Radiol. Sci.

• TABUCHI HIROYOSHI

  Umetani Precison Co.

• YAMAMOTO MIKIO

  National Institute of Radiological Sciences

• SATO KOKI

  National Institute of Radiological Sciences

• Furukawa Akira

  Radiation Hazards Research Group Nirs

関連論文

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• Chromosome aberrations in F1 embryos derived from male medaka germ cells exposed to γ-rays
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• Cytogenetic study on weakly irradiated cases in Tokai-mura criticality accident.
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• Radiation-induced Apoptosis Responsible for Limb Teratogenesis in Embryonic Mice
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• 249 Prenatal Radiation-Induced Teratogenesis and Adaptive Response Modified by p53 and General Caspase Inhibitors(Apoptosis related, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
• Radiation-Induced Teratogenesis and Adaptive Response in the Late Period of Organogenesis in Mice : I. Dose Rate Effects
• Effect of Smoking on Chromosomes Compared with That of Radiation in the Residents of a High-Background Radiation Area in China
• Imperceptible Effect of Radiation Based on Stable Type Chromosome Aberrations Accumulated in the Lymphocytes of Residents in the High Background Radiation Area in China
• O-06. Chromosome aberrations in the lymphocytes of residents in the high background radiation area in Guangdong province in China(Abstracts of the oral and poster presentations, Abstracts of the 54th Annual Meeting of the Society of Chromosome Research)
• Chromosome Translocation in Residents of the High Background Radiation Areas in Southern China
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• Effects of Caspase Inhibitors on Nuclear Protein NP95 Depletion Associated with Radiation-induced Apoptosis in Murine ThymicLymphoma 3SB Cells.
• Involvement of Protein Kinase C in Radiation-induced Apoptosis Regulation in 3SBH5 Cells
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• P-14. Aneuploidy caused by chromosomes showing premature centromere separation in a woman living in the high natural radiation area in Ramsar, Iran(Abstracts of the oral and poster presentations)
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• Rapid Decrease in the Expression of a Nuclear Protein NP95 Associated with Radiation-induced Apoptosis inMurine Thymic Lymphoma 3SB Cells.
• Involvement of Caspases in the Radiation-induced Apoptosis of Radiosensitive Murine Thymoma 3SB Cells
• Implication of Protein Kinase C Activation in the Radiation-Induced Apoptosis in Radiosensitive Murine Thymoma Cell 3SB
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• Rescue of Lethally Irradiated Mice from Hematopoietic Death by Pre-expousure to 0.5 Gy X-rays withoutRecovery from Peripheral Blood Cell Depletion
• Fas-independent Apoptosis Induced by UVC in p53-Mutated Human Epithelial Tumor A431 Cells through Activation of Caspase-8 and JNK/SAPK
• Radioadaptation in Embryogenesis Is Related to p53 Gene Status
• Long Term Effects Observed in Mice Rescued by 0.5 Gy Pre-exposure from Lethal Irradiation
• UVC-induced Apoptosis in Human Epithelial Tumor A431 Cells : Sequence of Apoptotic Changes and Involvement of Caspase (-8 and -3) Cascade
• Radioadaptation in Embryogenesis during Late Period of Organogenesis and Postnatal Retardation in the Prenatally Primed Mice
• OK432-lnduced Modification of Radioadaptive Response in Mice
• Modification of Acquired Radio-Resistance in Mice by OK432
• Chromosome aberrations in the lymphocytes of the patients with uterine cancer who received heavy-ion radiation therapy by HIMAC.
• Modification of Radiation-Induced Apoptosis Affects the Severity of Limb Teratogenesis in Embryonic Mice
• Cytogenetical Dose Estimation for 3 Severely Exposed Patients in the JCO Criticality Accident in Tokai-mura
• Comparison of Two Types of Coated Slide Glass Using a New Autofocusing Method for Microscopic Image of Chromosomes
• Effect of Estradiol on Radiation-induced Chromosome Aberrations in Human Lymphocytes
• 61 Visualization and Structural Analysis of Ring Chromosomes by Atomic Force Microscope(Chromosome aberrations, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
• Non-fluorescent chromosome painting method using peroxidase/diaminobenzidine reaction(Abstracts of the 48th Annual Meeting of the Society of Chromosome Research)
• Dynamic Analysis of Chromosome Aberrations in Three Patients of Tokai-mura Criticality Accident
• Cytogenetical study on the new myeloproliferative disorder, L-8313, found in the irradiated mouse.

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