Microarray Analysis of Differentially Expressed Genes in the Kidneys and Testes of Mice after Long-term Irradiation with Low-dose-rate γ-rays

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概要

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• Measuring global gene expression using cDNA or oligonucleotide microarrays is an effective approach to understanding the complex mechanisms of the effects of radiation. However, few studies have been carried out that investigate gene expression in vivo after prolonged exposure to low-dose-rate radiation. In this study, C57BL/6J mice were continuously irradiated with γ-rays for 485 days at dose-rates of 0.032-13 μGy/min. Gene expression profiles in the kidney and testis from irradiated and unirradiated mice were analyzed, and differentially expressed genes were identified. A combination of pathway analysis and hierarchical clustering of differentially expressed genes revealed that expression of genes involved in mitochondrial oxidative phosphorylation was elevated in the kidney after irradiation at the dose-rates of 0.65 μGy/min and 13 μGy/min. Expression of cell cycle-associated genes was not profoundly modulated in the kidney, in contrast to the response to acute irradiation, suggesting a threshold in the dose-rate for modulation of the expression of cell cycle-related genes in vivo following exposure to radiation. We demonstrated that changes to the gene expression profile in the testis were largely different from those in the kidney. The Gene Ontology categories "DNA metabolism", "response to DNA damage" and "DNA replication" overlapped significantly with the clusters of genes whose expression decreased with an increase in the dose-rate to the testis. These observations provide a fundamental insight into the organ-specific responses to low-dose-rate radiation.

• 日本放射線影響学会の論文
• 2009-05-16

著者

• Magae Junji

  Nuclear Technol. Res. Lab. Radiation Safety Res. Center Central Res. Inst. Of Electric Power Ind.

• Tanaka Kimio

  Department Of Physics School Of Medicine Sapporo Medical University

• Ono Tetsuya

  東北大学 生命科学研究科

• Ono T

  Department Of Cell Biology Graduate School Of Medicine Tohoku University

• Tanaka Kimio

  Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences

• UEHARA Yoshihiko

  Tohoku University, Graduate School of Medicine

• NAKAJIMA Tetsuo

  Radiation Effect Mechanisms Research Group, National Institute of Radiological Sciences

• WANG Bing

  Radiation Effect Mechanisms Research Group, National Institute of Radiological Sciences

• MATSUMOTO Tsuneya

  Institute for Environmental Sciences

• OGHISO Yoichi

  Institute for Environmental Sciences

• TANAKA Kimio

  Institute for Environmental Sciences

• ICHINOHE Kazuaki

  Institute for Environmental Sciences

• NAKAMURA Shingo

  Institute for Environmental Sciences

• TANAKA Satoshi

  Institute for Environmental Sciences

• NENOI Mitsuru

  Radiation Effect Mechanisms Research Group, National Institute of Radiological Sciences

• Wang Bing

  National Institute of Radiological Sciences

• NENOI Mitsuru

  National Institute of Radiological Sciences

• Wang Bing

  Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences

• Tanaka K

  Department Of Physics School Of Medicine Sapporo Medical University

• Tanaka K

  Department Of Radiobiology Research Institute For Environmental Sciences

• Tanaka Kimio

  Dep. Of Radiobiology Inst. For Environmental Sciences

• TAKI Keiko

  Radiation Effect Mechanisms Research Group, National Institute of Radiological Sciences

• WU Jianyu

  Radiation Effect Mechanisms Research Group, National Institute of Radiological Sciences

• ONO Tetsuya

  Graduate School of Medicine, Tohoku University

• UEHARA Yoshihiko

  Graduate School of Medicine, Tohoku University

• KAKIMOTO Ayana

  Radiation Effect Mechanisms Research Group, National Institute of Radiological Sciences

• Nakajima Tetsuo

  Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences

• Nakajima Tetsuo

  Radiat. Hazards Res. Group Nirs

• Oghiso Yoichi

  国立がんセンター研究所 がん予防基礎研究プロジェクト

• Oghiso Yoichi

  Internal Radiation Effects Research Group National Institute Of Radiological Sciences

• Wu Jianyu

  Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences

• Kakimoto Ayana

  Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences

• Wang B

  Research Center For Radiation Safety Natl. Inst. Radiol. Sci.

• Oghiso Yoichi

  Div. Radiotoxicol. Protect. Natl. Inst. Radiol. Sci.

• Ono Tetsuya

  Graduate School Of Medicine Tohoku Univ.

• Magae Jyunji

  Nuclear Technology Research Laboratory Radiation Safety Research Center Central Research Institute O

• Nenoi Mitsuru

  Radiat. Hazards Res. Gr. Natl. Inst. Radiol. Sci.

• Nenoi Mitsuru

  Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences

• Nakajima Tetsuo

  Radiat. Hazards Res. G. Nirs

• Nakajima Tetsuo

  Division Of Biology And Oncology National Institute Of Radiological Sciences

• Tanaka Kimio

  Dept. Of Cancer Cytogenet. Res. Inst. Rad. Biol. &med. Hiroshima University.

• Uehara Yoshihiko

  Tohoku University Graduate School Of Medicine

• Wang Bing

  Univ. Tokyo School Of Sci. Zoological Institute

• Ono Takashi

  Research Center Of Health Physical Fitness And Sports Nagoya University

• Tanaka Kimio

  Res. Inst. Nuclear Med. Biol. Hiroshima Univ.

• Tanaka Satoshi

  Institute for Biosciences, Mukogawa Women's University

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