Transcription Factor-recognition Sequences Potentially Involved in Modulation of Gene Expression after Exposure to Low-dose-rate γ-rays in the Mouse Liver
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概要
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In vivo modulation of gene expression profiles after low-dose and low-dose-rate irradiation has been observed in a variety of experimental systems. However, few studies actually investigated the underlying mechanisms for these genetic responses. In this study, we used pre-existing microarray data and searched for gene modulations in response to long-term, low-dose-rate irradiation. Nucleotide sequences in the neighboring region of the up-regulated, down-regulated, and unaffected genes were retrieved from the Entrez Gene database, and recognition sequences for transcription factors (TFs) were searched using the TFSEARCH database. As a result, we suggested 21 potential TF-binding sites with significantly different incidence between the three gene groups (up-regulated, down-regulated and unaffected gene groups). The binding sites for sterol regulatory element-binding protein 1 (SREBP-1), aryl hydrocarbon receptor (AhR/Ar) and olfactory 1 (Olf-1) were suggested to be involved in up-regulation, while the binding sites for glucocorticoid receptor (GR(GGTACAANNT GTYCTK) ) and hepatocyte nuclear factor 1 (HNF-1) were suggested to be involved in down-regulation of the genes. In addition, the binding sites for activating enhancer-binding protein 4 (AP-4), nuclear factor-κB (NFκB), GR (NNNNNNCNNTNTGTNCTNN) and early growth response 3 (Egr-3) were correlated with modulation of gene expression regardless of the direction of modulation. Our results suggest that these TF-binding sites are involved in gene modulations after long-term continuous irradiation with low-dose-rate γ rays. GR and/or SREBP-1 might be associated with the altered metabolic process observed in liver after exposure to low-dose-rate irradiation.
著者
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VARES Guillaume
Radiation Effect Mechanisms Research Group, National Institute of Radiological Sciences
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UEHARA Yoshihiko
Tohoku University, Graduate School of Medicine
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ONO Tetsuya
Tohoku University, Graduate School of Medicine
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NAKAJIMA Tetsuo
Radiation Effect Mechanisms Research Group, National Institute of Radiological Sciences
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WANG Bing
Radiation Effect Mechanisms Research Group, National Institute of Radiological Sciences
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TAKI Keiko
Clinical Genetic Oncology, Cancer Institute Hospital, Japanese Foundation for Cancer Research
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MATSUMOTO Tsuneya
Institute for Environmental Sciences
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OGHISO Yoichi
Institute for Environmental Sciences
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TANAKA Kimio
Institute for Environmental Sciences
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ICHINOHE Kazuaki
Institute for Environmental Sciences
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NAKAMURA Shingo
Institute for Environmental Sciences
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TANAKA Satoshi
Institute for Environmental Sciences
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NENOI Mitsuru
Radiation Effect Mechanisms Research Group, National Institute of Radiological Sciences
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