Trp53 Activity Is Repressed in Radio-adapted Cultured Murine Limb Bud Cells
スポンサーリンク
概要
- 論文の詳細を見る
Understanding the effects of of ionizing radiation (IR) at low dose in fetal models is of great importance, because the fetus is considered to be at the most radiosensitive stage of the development and prenatal radiation might influence subsequent development. We previously demonstrated the existence of an adaptive response (AR) in murine fetuses after pre-exposure to low doses of X-rays. Trp53-dependent apoptosis was suggested to be responsible for the teratogenic effects of IR; decreased apoptosis was observed in adapted animals. In this study, in order to investigate the role of Trp53 in AR, we developed a new model of irradiated micromass culture of fetal limb bud cells, which replicated proliferation, differentiation and response to IR in murine embryos. Murine fetuses were exposed to whole-body priming irradiation of 0.3 Gy or 0.5 Gy at embryonic day 11 (E11). Limb bud cells (collected from digital ray areas exhibiting radiation-induced apoptosis) were cultured and exposed to a challenging dose of 4 Gy at E12 equivalent. The levels of Trp53 protein and its phosphorylated form at Ser18 were investigated. Our results suggested that the induction of AR in mouse embryos was correlated with a repression of Trp53 activity.
- 2011-11-16
著者
-
Tanaka Kimio
Department Of Physics School Of Medicine Sapporo Medical University
-
Tanaka Kimio
Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences
-
VARES Guillaume
Radiation Effect Mechanisms Research Group, National Institute of Radiological Sciences
-
Vares Guillaume
National Institute of Radiological Sciences
-
Wang Bing
National Institute of Radiological Sciences
-
SHANG Yi
National Institute of Radiological Sciences
-
NENOI Mitsuru
National Institute of Radiological Sciences
-
Wang Bing
Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences
-
Tanaka K
Department Of Physics School Of Medicine Sapporo Medical University
-
Tanaka K
Department Of Radiobiology Research Institute For Environmental Sciences
-
Tanaka Kimio
Dep. Of Radiobiology Inst. For Environmental Sciences
-
Tanaka Kaoru
Radiation Hazards Research Group Natl. Inst. Radiol. Sci.
-
Wang B
Research Center For Radiation Safety Natl. Inst. Radiol. Sci.
-
Fujita Kazuko
Radiat. Hazards Res. Group Nirs
-
Shang Yi
Radiat. Hazards Res. Group Nirs
-
Hayata Isamu
Radiat. Hazards Res. Gr. Natl. Inst. Radiol. Sci
-
Nenoi Mitsuru
Radiat. Hazards Res. Gr. Natl. Inst. Radiol. Sci.
-
Nenoi Mitsuru
Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences
-
Fujita Kazuko
Toho Univ. School Of Med. Dept. Biol.
-
Vares Guillaume
(独)放射線医学総合研究所
-
Fujita Kazuko
Toho Univ. Graduate Sch. Of Biomolecular Sci .
-
Hayata Isamu
Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences
-
Tanaka Kimio
Res. Inst. Nuclear Med. Biol. Hiroshima Univ.
-
Fujita Kazuko
Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences
関連論文
- Inverse Dose-rate-effects on the Expressions of Extra-cellular Matrix-related Genes in Low-dose-rate γ-ray Irradiated Murine Cells
- Radiation Dose Measurement by Electron Spin Resonance Studies of Tooth Enamel in Lime and Non-lime Consuming Individuals from the Silchar Region of Northeast India
- Termisaltan Produced Regression of Left Ventricular Hypertrophy with the Improvement of Myocardial Perfusion and Fatty Acid Metabolism(Hypertension, Clinical 3 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Transcription Factor-recognition Sequences Potentially Involved in Modulation of Gene Expression after Exposure to Low-dose-rate γ-rays in the Mouse Liver
- Gene Silencing of Tead3 Abrogates Radiation-induced Adaptive Response in Cultured Mouse Limb Bud Cells
- Microdosimetry on a Mini-Reactor UTR-KINKI for Educational Uses and Biological Researches
- Microdosimetric Evaluation of Secondary Particles in a Phantom Produced by Carbon 290 MeV/nucleon Ions at HIMAC
- Effects of Sunlight Exposure on the Human Tooth Enamel ESR Spectra Used for Dose Reconstruction
- ヒトリンパ芽球由来細胞における放射線適応応答の分子機構に関する研究
- Coronary artery calcification detected by computed tomography is not related with angiographic stenosis grade for asymptomatic subjects
- PJ-036 Homogeneous washout pattern of 123I-MIBG images before beta-blocker therapy may predict the clinical outcome in patients with chronic heart failure(Nuclear Cardiology 2 (I) : PJ6)(Poster Session (Japanese))
- Mutation of RAS Oncogene in Atomic Bomb Radiation-exposed Leukemia
- Chromosomal Instability in Acute Myelocytic Leukemia and Myelodysplastic Syndrome Patients among Atomic Bomb Survivors
- Acute Myelogenous Leukemia With PIG-A Gene Mutation Evolved From Aplastic Anemia-Paroxysmal Nocturnal Hemoglobinuria Syndrome
- Microarray Analysis of Differentially Expressed Genes in the Kidneys and Testes of Mice after Long-term Irradiation with Low-dose-rate γ-rays
- Induction of Rhodanese, a Detoxification Enzyme, in Livers from Mice after Long-term Irradiation with Low-dose-rate Gamma-rays
- 66 Tissue specific mitochondrial DNA deletions in aged and irradiated mice(Radiation sensitivities in cells & tissues, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
- Miochondrial DNA Deletions and Aging induced by Low Dose Rate of Cs γ-ray in Mice
- Unstable-type Chromosome Aberrations in Lymphocytes from Individuals Living near Semipalatinsk Nuclear Test Site
- Interactions between cytoskeletal components during myoplasm rearrangement in ascidian eggs
- Cytogenetic and Molecular Changes in Leukemia among Atomic Bomb Survivors
- A gradient of radioactive contamination in Dolon village near the SNTS and comparison of computed dose values with instrumental estimates for the 29 August, 1949 nuclear test
- Comet Assay to Assess the Non-target Effect of Neutronradiation in Human Peripheral Blood
- Neutron-induced Adaptive Response Studied in Go Human Lymphocytes Using the Comet Assay
- High Incidence of Micronuclei in Lymphocytes from Residents of the Area near the Semipalatinsk Nuclear Explosion Test Site
- Neutron Energy-Dependent Initial DNA Damage and Chromosomal Exchange
- Radiation hormesis : Stimulatory effects of low doses ionizing radiation
- Chromosome aberrations and micronucleus in lymphocytes from residents of the area near the Semipalatinsknuclear explosion test site
- Chromosome and Micronucleus Aberrations in Lymphocytes from Resident Peoples in Semiparatinsk Nuclear Test Sites
- Chromosomal and Molecular Biological Changes in Atomic Bomb Radiation-related Leukemias, which were different from those in de novo Leukemias
- Effects of Neon Beam on Hematopoietic System in Mice
- Nuclear Abnormalities in Aspirated Thyroid Cells and Chromosome Aberrations in Lymphocytes of Residents Near the Semipalatinsk Nuclear Test Site
- A cell based assay for low dose rate γ-ray radiation respone of murine cells
- Radiation-induced Apoptosis Responsible for Limb Teratogenesis in Embryonic Mice
- Radiation Induced Apoptosis in the Mouse Limb Bud Cells in vivo and in vitro
- 249 Prenatal Radiation-Induced Teratogenesis and Adaptive Response Modified by p53 and General Caspase Inhibitors(Apoptosis related, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
- Radiation-Induced Teratogenesis and Adaptive Response in the Late Period of Organogenesis in Mice : I. Dose Rate Effects
- 79 Enhancement of Radiation-induced Mortality by Friend Leukemia Virus Infection : 2 Role of Radiosensitivity-Related Genes(Radiation sensitivities in cells & tissues, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
- Role of Radiosensitivity-Related Genes in Enhancement of Acute Radiation Effects by Retroviral Infection
- Appearance of p53-/-Thymocytes in p53+/-B10 Mice Irradiated for Thymic Lymphomagenesis
- Monosomy 7 and Jumping Translocation of ABL Oncogene in Radiation-induced Human Leukemias, detected by FISH Analysis
- ^Sr Concentration in Cow Teeth from South Ural Region, Russia, Using Monte Carlo Simulation
- Study on Influence of X-ray Baggage Scan on ESR Dosimetry for SNTS using Human Tooth Enamel
- Protein Expression of Cell Cycle Regulator, p27^, Correlates with Histopathological Grade of Non-Hodgkin's Lymphoma
- Instability of chromosome 7 in colony forming cells of patients with aplastic anemia
- Fluorescence in situ Hybridization Analysis of 12;21 Translocation in Japanese Childhood Acute Lymphoblastic Leukemia
- Detection of Residual Host Cells in Sex-mismatched Bone Marrow Transplantation in Various Hematological Diseases by Fluorescence in situ Hybridization
- Radiation Dose Estimation by Tooth Enamel EPR Dosimetry for Residents of Dolon and Bodene
- Results of EPR Dosimetry for Population in the Vicinity of the Most Contaminating Radioactive Fallout Trace After the First Nuclear Test in the Semipalatinsk Test Site
- Tooth Enamel EPR Dosimetry : Optimization of EPR Spectra Recording Parameters and Effect of Sample Mass on Spectral Sensitivity
- Hidden genetic or chromosomal alterations in patients with acute myeloid leukemia showing a cytogenetically normal karyotype
- Cleavage of RAD51 protein during apotosis.
- Transcriptional regulation of the catalase gene in the hydrogen peroxide-resistant cell line, HP 100
- 放射線適応応答に関与する多様な因子
- Effects of Caspase Inhibitors on Nuclear Protein NP95 Depletion Associated with Radiation-induced Apoptosis in Murine ThymicLymphoma 3SB Cells.
- Involvement of Protein Kinase C in Radiation-induced Apoptosis Regulation in 3SBH5 Cells
- Low-Dose Irradiation Mediates Ca^ Signaling in Cultured Rat Hepatocytes
- 8 Profiling of low-dose-rate γ-rays irradiation on the gene expression in murine cells by microarray(Low dose and low dose-rate effects, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
- Rapid Decrease in the Expression of a Nuclear Protein NP95 Associated with Radiation-induced Apoptosis inMurine Thymic Lymphoma 3SB Cells.
- Involvement of Caspases in the Radiation-induced Apoptosis of Radiosensitive Murine Thymoma 3SB Cells
- Implication of Protein Kinase C Activation in the Radiation-Induced Apoptosis in Radiosensitive Murine Thymoma Cell 3SB
- Mechanisms for Radiation-induced Apoptosis in a Radiosensitive Murine Thymoma Cell Line 3SB
- 236 Distinct functions of protein kinase C subtypes in the regulation of radiation-induced apoptosis in murine thymic lymphoma cells(Apoptosis related, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
- Effect of 0.5 Gy Pre-expore on the PFC of Lethally Irradiated Mice
- Rescue of Lethally Irradiated Mice from Hematopoietic Death by Pre-expousure to 0.5 Gy X-rays withoutRecovery from Peripheral Blood Cell Depletion
- Fas-independent Apoptosis Induced by UVC in p53-Mutated Human Epithelial Tumor A431 Cells through Activation of Caspase-8 and JNK/SAPK
- Radioadaptation in Embryogenesis Is Related to p53 Gene Status
- Long Term Effects Observed in Mice Rescued by 0.5 Gy Pre-exposure from Lethal Irradiation
- UVC-induced Apoptosis in Human Epithelial Tumor A431 Cells : Sequence of Apoptotic Changes and Involvement of Caspase (-8 and -3) Cascade
- Radioadaptation in Embryogenesis during Late Period of Organogenesis and Postnatal Retardation in the Prenatally Primed Mice
- OK432-lnduced Modification of Radioadaptive Response in Mice
- Modification of Acquired Radio-Resistance in Mice by OK432
- OK432 Modification of Radio-Adaptive Response
- RELATIONSHIP OF ACCESSORY PATHWAY CONDUCTION TO THE ATRIAL VULNERABILITY IN WOLFF-PARKINSON-WHITE SYNDROME: COMPARISON BEFORE AND AFTER CATHETER ABLATION
- USEFULNESS OF INTRACARDIAC ELECTROGRAM RECORDINGS FOR DIAGNOSIS OF SINUS NODE FUNCTIONS IN SSS PATIENTS AND FOR ABLATION OF WPW SYNDROME AND ARIOVENTRICULAR NODE REENTRY TACHYCARDIA
- Analysis or Radioresistant Variants Derived from 3SB Cell
- P-10. Dose and dose-rate effects on dicentrics and translocations in mice spleen cells after continuous irradiation with low-dose-rate gamma-rays(Abstracts for the oral and the poster presentations,Abstracts of the Joint Meeting: the 58th Annual Meeting o
- Increase in mRNA and Protein Level of Spermidine/ Spermine Acetliransferase after X-ray Irradiation
- A New Method for improving Information Bit Rate and Average Bit Error Rate in Two Way Transmission Systems
- γ and α ray induced delayed chromosome instability in human B cell line
- Induction of Delayed Chromosome Instability in Human Hematopoietic Cells by Various Radiation Sources
- Detection of 9;22 translocation and monosomy 7 on interphase cells in atomic bomb related leukemias by fluorescence in situ bybridization
- Frequencies of ^Co γ rays and ^Cf neutron-induced chromosome translocations in human lymphocytes, detected by two colors fluorescence in situ hybridization of chromosome subsets.
- Design of the Transmission Line Simulator having a Certain Bit Error Rate ((放送方式、放送現業、無線・光伝送)2000 Asia-Pacific Symposium on Broadcasting and Communications)
- Statistical Propagation Characteristics for Land-Mobile Satellite Communication
- A Method for increasing Information Bit Error Rate and decreasing Delay Time in Burst Transfer System and Its Performance Evaluation(ABSTRACTS OF PROCEEDINGS OF THE SCHOOL OF INFORMATION TECHNOLOGY AND ELECTRONICS SERIES J TOKAI UNIVERSITY -2003-2004-)
- The Expression of the Protochordate Homologue of the Proteasome Regulatory Subunit Rpn12 is Transcriptionally and Post-translationally Regulated during Cleavage Stage(Developmental Biology)
- An alternatively spliced gene encoding a Y-box protein showing maternal expression and tissue-specific zygotic expression in the ascidian embryo
- Two Distinct Cell Types Identified in the Ascidian Notochord
- Effects of UVC-Irradiation on Cultured Mouse Limb Bud Cells
- 60 Energy Spectrum Dependent Chromosome Aberrations in Monoenergetic Neutrons(Chromosome aberrations, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
- Modification of Radiation-Induced Apoptosis Affects the Severity of Limb Teratogenesis in Embryonic Mice
- Design of Parallel Scrambler Applied to Digital Multiplexer
- Gene Silencing of Tead3 Abrogates Radiation-induced Adaptive Response in Cultured Mouse Limb Bud Cells
- Effects of Prenatal Exposure to Low-dose β Radiation from Tritiated Water on the Neurobehavior of Mice
- Transcription Factor-recognition Sequences Potentially Involved in Modulation of Gene Expression after Exposure to Low-dose-rate γ-rays in the Mouse Liver
- 低線量影響としての放射線適応応答 : 放射線防護へのインパクト
- The comparison of dose distributions in a water phantom for accelerator-based and reactor-based neutron irradiation fields for NCT.
- A Consideration about Neutron Irradiation Systems for Boron Neutron Capture Therapy using Accelerators and Research Reactors
- Trp53 Activity Is Repressed in Radio-adapted Cultured Murine Limb Bud Cells