Trp53 Activity Is Repressed in Radio-adapted Cultured Murine Limb Bud Cells

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概要

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• Understanding the effects of of ionizing radiation (IR) at low dose in fetal models is of great importance, because the fetus is considered to be at the most radiosensitive stage of the development and prenatal radiation might influence subsequent development. We previously demonstrated the existence of an adaptive response (AR) in murine fetuses after pre-exposure to low doses of X-rays. Trp53-dependent apoptosis was suggested to be responsible for the teratogenic effects of IR; decreased apoptosis was observed in adapted animals. In this study, in order to investigate the role of Trp53 in AR, we developed a new model of irradiated micromass culture of fetal limb bud cells, which replicated proliferation, differentiation and response to IR in murine embryos. Murine fetuses were exposed to whole-body priming irradiation of 0.3 Gy or 0.5 Gy at embryonic day 11 (E11). Limb bud cells (collected from digital ray areas exhibiting radiation-induced apoptosis) were cultured and exposed to a challenging dose of 4 Gy at E12 equivalent. The levels of Trp53 protein and its phosphorylated form at Ser18 were investigated. Our results suggested that the induction of AR in mouse embryos was correlated with a repression of Trp53 activity.

• 2011-11-16

著者

• Tanaka Kimio

  Department Of Physics School Of Medicine Sapporo Medical University

• Tanaka Kimio

  Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences

• VARES Guillaume

  Radiation Effect Mechanisms Research Group, National Institute of Radiological Sciences

• Vares Guillaume

  National Institute of Radiological Sciences

• Wang Bing

  National Institute of Radiological Sciences

• SHANG Yi

  National Institute of Radiological Sciences

• NENOI Mitsuru

  National Institute of Radiological Sciences

• Wang Bing

  Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences

• Tanaka K

  Department Of Physics School Of Medicine Sapporo Medical University

• Tanaka K

  Department Of Radiobiology Research Institute For Environmental Sciences

• Tanaka Kimio

  Dep. Of Radiobiology Inst. For Environmental Sciences

• Tanaka Kaoru

  Radiation Hazards Research Group Natl. Inst. Radiol. Sci.

• Wang B

  Research Center For Radiation Safety Natl. Inst. Radiol. Sci.

• Fujita Kazuko

  Radiat. Hazards Res. Group Nirs

• Shang Yi

  Radiat. Hazards Res. Group Nirs

• Hayata Isamu

  Radiat. Hazards Res. Gr. Natl. Inst. Radiol. Sci

• Nenoi Mitsuru

  Radiat. Hazards Res. Gr. Natl. Inst. Radiol. Sci.

• Nenoi Mitsuru

  Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences

• Fujita Kazuko

  Toho Univ. School Of Med. Dept. Biol.

• Vares Guillaume

  (独)放射線医学総合研究所

• Fujita Kazuko

  Toho Univ. Graduate Sch. Of Biomolecular Sci .

• Hayata Isamu

  Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences

• Tanaka Kimio

  Res. Inst. Nuclear Med. Biol. Hiroshima Univ.

• Fujita Kazuko

  Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences

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