Comprehensive Search for X-ray-responsive Elements and Binding Factors in the Regulatory Region of the GADD45a Gene
スポンサーリンク
概要
- 論文の詳細を見る
The growth arrest and DNA damage-inducible protein 45alpha (GADD45a) gene is responsive to a variety of DNA-damaging agents. It is known that induction of the GADD45a gene is regulated in a p53-dependent manner after ionizing irradiation. Our previous study showed that X-ray irradiation increased the transcription rate of the GADD45a gene much earlier than the maximum accumulation of stabilized p53 protein in human myeloblastic leukemia ML-1 cells. We hypothesized that some transcription factor(s) may cooperate with p53 in regulating the GADD45a gene early after the irradiation of ML-1 cells. This idea is supported by recent studies showing that the p53-dependent activation of several genes in human and mouse cells requires some additional transcription factors, such as Sp1, GKLF, Ets1, and IRF-1. To examine the possible involvement of cooperating factors in transcriptional regulation of the GADD45a gene by ionizing radiation, we comprehensively searched for the X-ray-inducible binding locus of the nuclear factor throughout the upstream region (-2244bp/+89bp) and the third intron (+1389bp/+2488bp) of the GADD45a gene by EMSA using 136 probes. The X-ray-responsive binding of nuclear factors was detected at eight loci. Oct, NF-κB, HNF, NF-AT, and KLF family transcription factors were identified by a competition assay. It is possible that some of these factors cooperate with p53 to mediate transcriptional regulation of the GADD45a gene after ionizing irradiation.
- 日本放射線影響学会の論文
- 2003-12-15
著者
-
NENOI Mitsuru
Radiation Effect Mechanisms Research Group, National Institute of Radiological Sciences
-
Daino Kazuhiro
Life And Bioresourse Graduate School Of Science And Technology Chiba University
-
ICHIMURA Sachiko
Radiat. Hazards Res. Gr., Natl. Inst. Radiol. Sci.
-
Nenoi Mitsuru
Radiat. Hazards Res. Gr. Natl. Inst. Radiol. Sci.
-
Ichimura Sachiko
Radiat. Hazards Res. Gr. Natl. Inst. Radiol. Sci.
-
Ichimura Sachiko
Radiation Hazards Research Group National Institute Of Radiological Sciences
関連論文
- Transcription Factor-recognition Sequences Potentially Involved in Modulation of Gene Expression after Exposure to Low-dose-rate γ-rays in the Mouse Liver
- Microarray Analysis of Differentially Expressed Genes in the Kidneys and Testes of Mice after Long-term Irradiation with Low-dose-rate γ-rays
- Induction of Rhodanese, a Detoxification Enzyme, in Livers from Mice after Long-term Irradiation with Low-dose-rate Gamma-rays
- 194 Radiation-induced Germ-line Mutations Detected by a Direct Comparison of Parents and Children DNA Sequences Containing SNPs(Mutation, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
- Analysis of Radiation-induced Germline Mutation Using SNP Marker
- Characterization of the human spermidine/spermine N1-acetyltransferase gene promoter
- Transcriptional regulation of the catalase gene in the hydrogen eroxide-resistant cell line, HP100
- A novel radiation resposible protein regulated by alternative splicing of spermidine/ spermine N1-acetyltransferase gene
- Transcriptional regulation of the catalase gene in the hydrogen peroxide-resistant cell line, HP100
- Comparison of the 5' upstream region of the evolutionarily equivalent polyubiquitin gene of humans and Chinese hamsters
- Structure of the human ubiquitin gene UbC
- A sequence analysis of the promoter of the human ubiquitin gene UbC
- CHARACTERIZATION OF THE GROWTH ARREST AND DNA DAMAGE-INDUCIBLE PROTEIN 45α IN MEDAKA FISH(Genetics,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- 146 Construction of low-dose radiation-responsive reporter vector(Signal transduction, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
- 11 Identification of transcription factor binds to upstream region of the GADD45 gene after ionizing irradiation(Low dose and low dose-rate effects, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
- Comprehensive Search for X-ray-responsive Elements and Binding Factors in the Regulatory Region of the GADD45a Gene
- Transcription factors binding to the cis-elements of GADD45 gene in ML-1 cells after low-dose irradiation
- Run-On Analysis of Activation of Mammalian DNA Damage-Inducible Genes by Low-Dose of X-Rays
- Transcription Factor-recognition Sequences Potentially Involved in Modulation of Gene Expression after Exposure to Low-dose-rate γ-rays in the Mouse Liver
- Trp53 Activity Is Repressed in Radio-adapted Cultured Murine Limb Bud Cells