Neutron-induced Adaptive Response Studied in Go Human Lymphocytes Using the Comet Assay
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概要
- 論文の詳細を見る
This study demonstrates that cells adapted to ionizing radiation developed reduced initial DNA damage when compared to non-adapted cells. The results were obtained by subjecting in vitro irradiated whole blood from 10 healthy volunteers (including 2 A-bomb survivors carrying 1.5-2 Gy in vivo exposure) in an unstimulated condition (G_0) using the comet assay. The intensity of DNA damage was assessed by computing the `tail moment'. Adaptive response (AR) was noticed in only donor 3, as indicated by reduced tail moment when the blood samples received priming + challenging doses over a 4 h interval. The priming dose was either 0.01 Gy ^<137>Cs γ-rays or 0.0025 Gy ^<252>Cf neutrons. The delivered challenging dose was either 1 Gy ^<60>Co g-rays or 0.25 Gy ^<252>Cf neutrons. The irradiation was conducted using the HIRRAC facility. A prior exposure to 0.0025 Gy ^<252>Cf neutrons nullified the excess tail moment caused by 0.25 Gy neutrons given during a 4 h gap. In a similar way, 0.01 Gy ^<137>Cs γ-rays offered a cross-adaptive response to the neutron challenging dose. The tail moment of A-bomb survivors after in vitro irradiation was less than that of the age-matched control and, at the same time, was not influenced by the priming dose. An altered subset and the immunological status of blood after A-bomb exposure were cited as possible factors. Because AR can affect the outcome of RBE, its individual variability only emphasizes the need to have individual biodosimetry for better risk assessment, especially in planning for a long space voyage.
- 日本放射線影響学会の論文
著者
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Tanaka Kimio
Department Of Physics School Of Medicine Sapporo Medical University
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Tanaka Kimio
Radiation Effect Mechanisms Research Group National Institute Of Radiological Sciences
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KAMADA NANAO
Department of Cancer Cytogenetics
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Kamada N
Department Of Cancer Cytogenetics Research Institute For Radiation Biology And Medicine Hiroshima Un
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Kamada Nanao
広島大学原爆放射能医学研究所
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Kamada Nanao
Hiroshima Atomic Bomb Survivors Relief Foundation
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Tanaka K
Department Of Physics School Of Medicine Sapporo Medical University
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Tanaka K
Department Of Radiobiology Research Institute For Environmental Sciences
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Tanaka Kimio
Dep. Of Radiobiology Inst. For Environmental Sciences
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Tanaka Kimio
Department Of Biology Faculty Of Science Konan University
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Kamada Nanao
Research Institute For Radiation Biology &medicine Hiroshima Univ.
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Kamada Nanao
Department Of Hematology Research Institute For Nuclear Medicine & Biology Hiroshima University
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GAJENDIRAN NATARAJAN
Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima
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KUMARAVEL THIRUKAZHUKUNDRAM
Laboratory of Molecular Genetics, GRC, NIA, NIH
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Kumaravel Thirukazhukundram
Laboratory Of Molecular Genetics Grc Nia Nih
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Gajendiran Natarajan
Department Of Cancer Cytogenetics Research Institute For Radiation Biology And Medicine Hiroshima Un
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Kamada Nanao
Dept. Of Cancer Cytogenet. Res. Inst. Rad. Biol. &med. Hiroshima University.
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Tanaka Kimio
Res. Inst. Nuclear Med. Biol. Hiroshima Univ.
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