The Expression of the Protochordate Homologue of the Proteasome Regulatory Subunit Rpn12 is Transcriptionally and Post-translationally Regulated during Cleavage Stage(Developmental Biology)

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In order to identify the maternal mRNAs which have important roles in the very early stage of embryogenesis, a Ciona intestinalis 64-cell stage cDNA library was subtracted from an unfertilized egg cDNA library. We thereby cloned Cipros1, which encodes the protochordate homologue of the proteasome regulatory subunit Rpn12. Neither Cipros1 mRNA nor Cipros1 protein showed any spatial localization. However, Cipros1 mRNA was expressed at a level at least five-times higher in unfertilized eggs and about two-times higher in cleavage stage embryos, than in other embryonic stages. In unfertilized eggs, Cipros1 protein was expressed at a level about twice as higher as during the other stages. Moreover, minor, smaller isoforms of Cipros1 were expressed specifically in unfertilized eggs and during early cleavage stages. Since a single Cipros1 transcript was detected throughout the development, these smaller isoforms might be generated post-translationally.
社団法人日本動物学会の論文
1999-02-15