Fluorescent in situ hybridization to ascidian chromosomes
スポンサーリンク
概要
- 論文の詳細を見る
The draft genome of the ascidian Ciona intestinalis has been sequenced. Mapping of the genome sequence to the Ciona 14 haploid chromosomes is essential for future studies of the genome-wide control of gene expression in this basal chordate. Here we describe an efficient protocol for fluorescent in situ hybridization for mapping genes to the Ciona chromosomes. We demonstrate how the locations of two BAC clones can be mapped relative to each other. We also show that this method is efficient for coupling two so-far independent scaffolds into one longer scaffold when two BAC clones represent sequences located at either end of the two scaffolds.
- 社団法人日本動物学会の論文
- 2004-02-25
著者
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SATOH Nori
Department of Zoology, Graduate School of Science, Kyoto University
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Satoh N
Department Of Zoology Graduate School Of Science Kyoto University
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Satoh Nori
Okinawa Inst. Sci. And Technol. Promotion Corp. Okinawa Jpn
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Yoshizaki Fumiko
Department of Biological Science, Graduate School of Science, the University of Tokyo
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Satou Yutaka
Department of Zoology, Graduate School of Science, Kyoto University
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Shoguchi Eiichi
Department of Zoology, Graduate School of Science, Kyoto University
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Saiga Hidetoshi
Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University
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NISHIKATA Takahito
Department of Biology, Faculty of Science, Konan University
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Ikuta Tetsuro
Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University
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Asano Katsutoshi
Nihon Gene Research Laboratories
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Ikuta Tetsuro
Department Of Biological Sciences Graduate School Of Science Tokyo Metropolitan University
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Satoh Nori
Departmant Of Zoology Graduate School Of Science Kyoto University
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Saiga H
Division Of Biological Sciences Tokyo Metropolitan University
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Satou Y
Department Of Zoology Graduate School Of Science Kyoto University
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Satou Yutaka
Department Of Zoology Graduate School Of Science Kyoto University
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Saiga Hidetoshi
Department Of Biological Sciences Graduate School Of Science Tokyo Metropolitan University
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Shoguchi E
Department Of Zoology Graduate School Of Science Kyoto University
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Shoguchi Eiichi
Department Of Zoology Graduate School Of Science Kyoto University
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Nishikata Takahito
Department Of Biology Faculty Of Science And Engineering And High Technology Research Center Konan U
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Yoshizaki Fumiko
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Satou Yutaka
Department Of Zoology Faculty Of Science Kyoto University
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Yoshizaki Fumiko
Department of Biological Sciences, Graduate School of Science, University of Tokyo
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