Isolation and characterization of cDNA clones for epidermis-specific and muscle-specific genes in Ciona savignyi embryos
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概要
- 論文の詳細を見る
Ascidian eggs and embryos have provided an appropriate experimental system to explore the cellular and molecular mechanisms involved in the embryonic cell specification and pattern formation of the embryo. In Japan, most of the studies of ascidian embryology have been carried out with the large eggs of Halocynthia roretzi. However, for future studies, Ciona species may provide a better experimental system, in particular with respect to the incorporation of genetic approaches. In order to establish Ciona as an experimental system, molecular markers with which to examine cellular differentiation are required. In the present study, we isolated and characterized cDNA clones for two epidermis-specific genes (CsEpi-1 and CsEpi-2) and for two muscle-specific genes (CsMA-1 and CsMu-1). CsEpi-1 encodes a polypeptide with three trefoil domains, while CsMA-1 encodes a muscle-type actin from C. savignyi Although CsEpi-2 and CsMu-1 transcripts seem to have a poly(A) tail at the 3' end, we could not find a distinct open reading frame in the sequences. Probes for CsEpi-1, CsMA-1 and CsMu-1 cross-reacted with C. intestinalis embryos. These cDNAs are useful as molecular markers for the specification of epidermis and muscle of Ciona embryos.
- 社団法人日本動物学会の論文
著者
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CHIBA Shota
Department of Frontier Materials Chemistry, Graduate School of Science and Technology, Hirosaki Univ
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Chiba Shota
Department Of Biology Faculty Of Science Konan University
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Chiba Shota
Department Of Zoology Graduate School Of Science Kyoto University:department Of Biology Konan Univer
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Satoh Noriyuki
Department of Biology, Niigata University
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Satou Yutaka
Department of Zoology, Graduate School of Science, Kyoto University
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NISHIKATA Takahito
Department of Biology, Faculty of Science, Konan University
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Satou Y
Department Of Zoology Graduate School Of Science Kyoto University
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Satou Yutaka
Department Of Zoology Graduate School Of Science Kyoto University
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Satoh Noriyuki
Department Of Biology Niigata University
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Nishikata Takahito
Department Of Biology Faculty Of Science And Engineering And High Technology Research Center Konan U
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Nishikata Takahito
Department Of Biology Konan University
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Satou Yutaka
Department Of Zoology Faculty Of Science Kyoto University
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- Preface
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