Construction of a cDNA microarray derived from the ascidian Ciona intestinalis
スポンサーリンク
概要
- 論文の詳細を見る
A cDNA microarray was constructed from a basal chordate, the ascidian Ciona intestinalis. The draft genome of Ciona has been read and inferred to contain similar to16,000 protein-coding genes, and cDNAs for transcripts of 13,464 genes have been characterized and compiled as the "Ciona intestinalis Gene Collection Release I". In the present study, we constructed a cDNA microarray of these 13,464 Ciona genes. A preliminary experiment with Cy3- and Cy5-labeled probes showed extensive differential gene expression between fertilized eggs and larvae. In addition, there was a good correlation between results obtained by the present microarray analysis and those from previous EST analyses. This first microarray of a large collection of Ciona intestinalis cDNA clones should facilitate the analysis of global gene expression and gene networks during the embryogenesis of basal chordates.
- 社団法人日本動物学会の論文
- 2003-10-25
著者
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SATOH Nori
Department of Zoology, Graduate School of Science, Kyoto University
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Ueno Naoto
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
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Fujie Manabu
Department Of Zoology Graduate School Of Science Kyoto University
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Ueno N
Laboratory Of Developmental Biology National Institute For Basic Biology
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TAKAHASHI Hiroki
Laboratory of Biomolecular Dynamics, Department of Physics, Kitasato University School of Science
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Satou Yutaka
Department of Zoology, Graduate School of Science, Kyoto University
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Azumi Kaoru
Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University
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Ueno Naoto
Laboratory Of Developmental Biology National Institute For Basic Biology
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Miki Yasufumi
CREST, Japan Science and Technology Corporation
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Usami Takeshi
Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University
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Ishikawa Hisayoshi
Department of Zoology, Graduate School of Science, Kyoto University
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Kitayama Atsusi
Laboratory of Developmental Biology, National Institute for Basic Biology
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Satoh Nori
Departmant Of Zoology Graduate School Of Science Kyoto University
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Satoh Nori
Department Of Zoology Graduate School Of Science Kyoto University:crest Japan Science And Technology
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Azumi Kaoru
Department Of Biochemistry Graduate School Of Pharmaceutical Sciences Hokkaido University:crest Japa
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Azumi Kaoru
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Hokkaido University
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Satou Yutaka
Department Of Zoology Graduate School Of Science Kyoto University
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Usami Takeshi
Department Of Biochemistry Graduate School Of Pharmaceutical Sciences Hokkaido University
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Miki Yasufumi
Crest Japan Science And Technology Corporation
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Kitayama Atsusi
Laboratory Of Developmental Biology National Institute For Basic Biology
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Ishikawa Hisayoshi
Department Of Zoology Graduate School Of Science Kyoto University
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Takahashi Hideya
Ushimado Marine Laboratory Faculty Of Science Okayama University
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Takahashi Hiroki
Laboratory Of Biomolecular Dynamics Department Of Physics Kitasato University School Of Science
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Satou Yutaka
Department Of Zoology Faculty Of Science Kyoto University
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Ishikawa Hisayoshi
Department Of Life Sciences Graduate School Of Bioresources Mie University
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Takahashi Hiroki
Ushimado Marine Biological Station, Okayama Univeristy
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