Association of a 66 kDa homolog of Chlamydomonas DC2, a subunit of the outer arm docking complex, with outer arm dynein of sperm flagella in the ascidian Ciona intestinalis
スポンサーリンク
概要
- 論文の詳細を見る
We previously identified a 66 kDa axonernal protein (Ci-Axp66.0) in sperm of the ascidian Ciona intestinalis. Here we found that Ci-Axp66.0 shows sequence similarity to the DC2 subunit of the Chiamydomonas outer arm docking complex. Analysis of secondary structure of Ci-Axp66.0 suggested that the N-terminal two-thirds of the molecule is rich in coiled coil structure, as in Chiamydomonas DC2. Immunogold localization revealed that it is located in the vicinity of outer arm dynein. Ci-Axp66.0 was partly extracted from the axonemes by a high salt solution and co-purified with outer arm dynein. This co-purification was not affected by the absence of Mg2l in isolation buffer, indicating that Ci-Axp66.0 is associated with outer arm dynein. These results suggest that Ci-Axp66.0 is a component of the outer arm dynein docking complex in the axonemes of Ciona sperm.
- 社団法人日本動物学会の論文
- 2006-08-25
著者
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SATOH Nori
Department of Zoology, Graduate School of Science, Kyoto University
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Satoh Nori
Dep. Of Zoology Graduate School Of Sci. Kyoto Univ.
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Ushimaru Yuji
Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku Univ
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Konno Aru
Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku Univ
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Kaizu Maiko
Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku Univ
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Ogawa Kazuo
Center for Radioisotope Facilities, Okazaki Research Facilities, National Institutes of Natural Scie
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Inaba Kazuo
Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku Univ
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Satoh Nori
Departmant Of Zoology Graduate School Of Science Kyoto University
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Konno Aru
Department Of Developmental Biology And Neurosciences Graduate School Of Life Sciences Tohoku Univer
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Kaizu Maiko
Department Of Developmental Biology And Neurosciences Graduate School Of Life Sciences Tohoku Univer
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Ushimaru Yuji
Department Of Developmental Biology And Neurosciences Graduate School Of Life Sciences Tohoku Univer
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Ogawa Kazuo
Center For Radioisotope Facilities Okazaki National Research Institutes
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