Functional and heterologous expression of human protein O-linked mannose β-1,2-N-acetylglucosaminyltransferase 1 in zebrafish(METHODS)
スポンサーリンク
概要
- 論文の詳細を見る
Although membrane-associated proteins are related to many diseases and are important targets for drug discovery, their expression is often difficult in bacterial hosts such as Escherichia coli. To overcome this limitation, here, we focused on a novel host-vector system in zebrafish for the expression of human protein O-linked mannose β-1,2-N-acetylglu-cosaminyltransferase I (hPOMGnT1) which is related to muscle-eye-brain disease. For the expression of hPOMGnT1, the vector pZex-EGFP-pXI-hPOMGnT1 was constructed and injected into fertilized eggs. Using this system, we demonstrated that recombinant hPOMGnT1 was successfully expressed in the whole bodies of zebrafish embryos.
著者
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Endo Tamao
Molecular Glycobiology Research Team For Mechanism Of Aging Tokyo Metropolitan Institute Of Gerontol
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Manya Hiroshi
Molecular Glycobiology Research Team For Mechanism Of Aging Tokyo Metropolitan Geriatric Hospital And Institute Of Gerontology
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Avsar-ban Eriko
Department Of Life Sciences Graduate School Of Bioresources Mie University
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Ishikawa Hisayoshi
Department Of Life Sciences Graduate School Of Bioresources Mie University
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Akiyama Shin'ichi
Department Of Nephrology Graduate School Of Medicine Nagoya University
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Tamaru Yutaka
Department of Life Sciences, Graduate School of Bioresources, Mie University
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Tamaru Yutaka
Department Of Life Sciences Graduate School Of Bioresources Mie University
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Endo Tamao
Molecular Glycobiology Research Team For Mechanism Of Aging Tokyo Metropolitan Geriatric Hospital And Institute Of Gerontology
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- 哺乳動物におけるO-マンノース型糖鎖の生合成
- Functional and heterologous expression of human protein O-linked mannose β-1,2-N-acetylglucosaminyltransferase 1 in zebrafish(METHODS)
- Femtosecond Pulsed Laser as a Microscalpel for Microdissection and Isolation of Specific Sections from Biological Samples
- Functional and heterologous expression of human protein O-linked mannose β-1,2-N-acetylglucosaminyltransferase 1 in zebrafish