Femtosecond Pulsed Laser as a Microscalpel for Microdissection and Isolation of Specific Sections from Biological Samples

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概要

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• The femtosecond laser is a candidate tool for dissecting and obtaining specific tissues or cells from biological samples. In this paper, we report on the dissection of flammable (heat-sensitive) plant tissues (Eucommia ulmoides stem) and that of a transparent zebrafish embryo placed in water. During laser dissection in the plant tissues, we observed that a low repetition rate of the irradiations is crucial for reducing the risk of thermal damage. During laser dissection of the zebrafish embryo, the laser-induced bubble formation at the tissue surface disturbed the laser-induced dissection. We concluded that the "interior laser dissection method", in which the dissection is performed by moving the laser spot inside a biological sample, is an efficient way to dissect a sample that is placed in water. In view of our findings, the advantages of fs laser dissection are discussed on the basis of the physical characteristics of fs laser machining.

• Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
• 2007-09-15

著者

• Akiyama Shin-ichi

  Department Of Cancer Chemotherapy Institute For Cancer Research

• UEDA Mitsuyoshi

  Graduate School of Agriculture, Kyoto University

• HIRAO Kazuyuki

  Graduate School of Engineering, Kyoto University

• Fukusaki Eiichiro

  Graduate School of Engineering, Osaka University

• Tamaru Yutaka

  Department Of Life Sciences Graduate School Of Bioresources Mie University

• Sakakura Masaaki

  JST, Innovation Plaza Kyoto, Kyoto 615-8245, Japan

• Tamaru Yutaka

  Department of Life Sciences, Facultiy of Bioresources, Mie University, Tsu 514-8507, Japan

• Kajiyama Shinichiro

  Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan

• Tsutsumi Masafumi

  Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan

• Si Jinhai

  Department of Electric Science and Technique, School of Electronics and Information Engineering, Xi'an Jiaotong University, Xianning-Xilu 28, Xi'an 710049, China

• Miura Kiyotaka

  Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan

• Si Jinhai

  Department of Electric Science and Technique, School of Electronics and Information Engineering, Xi'an Jiaotong University, Xianning-Xilu 28, Xi'an 710049, China

• Akiyama Shin-ichi

  Department of Life Sciences, Facultiy of Bioresources, Mie University, Tsu 514-8507, Japan

• Hirao Kazuyuki

  Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan

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