Effects of mechanical stress on the eryression of type XII collagen mRNA in human periodontal ligament cells

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概要

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• The aim of this study is to analyze the effect of mechanical stress on the formation and maintenance of the periodontal ligament(PDL). Type XII collagen was chosen as a characteristic marker of extracellular matrix, together with three other noncollagenous proteins, osteopontin, osteonectin and osteocalcin. Human PDL cells were cultured and subjected to intermittent tensile stress using a Flexercell Strain Unit^<TM>(1/20Hrz, 18% elongation for 1, 3, 5 or 7days). By the RT-PCR method with a LightCycler^<TM> mRNAs of these proteins were quaentitatively analyzed. It was found that the mRNA expression of collagen XII, osteocalcin and osteonectin was increased by the mechanical stress in a time-dependent manner, wheres no significant change of mRNA of osteopontin did not observed. The results suggested that intermittent stress positively contributes to the formation and maturation of PDL cells to form the PDL.

• 硬組織再生生物学会の論文

著者

• ABIKO Yoshihiro

  Department of Dental Science, Institute of Personalized Medical Science, Health Sciences University

• KAKU Tohru

  Department of Oral Pathology, School of Dentistry, Health Sciences University of Hokkaido

• Kawakami Toshiyuki

  Department Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Ueda Yasuo

  Dept. Of Oral Functional Science Graduate School Of Dental Medicine Hokkaido University

• Satoh Noriyuki

  Department of Biology, Niigata University

• KUBOKI Yoshinori

  Department of Oral Biochemistry, School of Dentistry, Hokkaido University

• Takita Hiroko

  Department Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Takita H

  Central Research Institute

• Takita Hiroko

  Department Of Biochemistry Hokkaido University Dental School

• Kaku T

  Department Of Oral Pathology School Of Dentistry Health Sciences University Of Hokkaido

• Kaku Tohru

  Department Of Oral Pathology Hokkaido Health Sciences University Of Hokkaido

• Kaku Tohru

  School Of Dentistry Health Sciences University Of Hokkaido

• Sato Noriyuki

  Dept. Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Ueda Yasuo

  Department Of Oral Functional Science Hokkaido University Graduate School Of Dental Medicine

• Kuboki Y

  Hokkaido University

• Kuboki Yoshinori

  Department Of Biochemistry Hokkaido University School Of Dentistry

• Kuboki Yoshinori

  Emeritus Professor Hokkaido University

• Ohata Noboru

  Department of Prosthetic Dentistry II, School of Dentistry, Hokkaido University

• Ohata Noboru

  Department Of Oral Functional Science Hokkaido University Graduate School Of Dental Medicine

• Uno Keiji

  Dept. Of Oral Functional Science Graduate School Of Dental Medicine Hokkaido University

• Kuboki Yoshinori

  Dept. Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Kuboki Yoshinori

  Dept Biochem Sch Dentistry Hokkaido University

• Ohata Noboru

  Dept. Of Oral Functional Science Graduate School Of Dental Medicine Hokkaido University

• Satoh Noriyuki

  Department Of Biology Niigata University

• Ukegawa Tetsuya

  Dept. of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University

• UNO Keiji

  Department of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University

• UKEGAWA Tetsuya

  Department of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University

• Ukegawa Tetsuya

  Dept. Of Oral Functional Science Graduate School Of Dental Medicine Hokkaido University

• Abiko Yoshihiro

  Division Of Clinical Oral Pathology Department Of Human Biology And Pathophysiology School Of Dentis

• Kaku Tohru

  Department Of Human Biology And Pathophysiology School Of Dentistry Health Sciences University Of Ho

• Abiko Yoshihiro

  Department Of Dental Science Institute Of Personalized Medical Science Health Sciences University Of

• Abiko Yoshihiro

  北海道医療大学･個体差医療科学センター 歯学部門

• Kaku Tohru

  Deparment Of Oral Pathology School Of Dentistry Health Sciences University Of Hokkaido

• Ohata Noboru

  Department Of Oral Functional Science Graduate School Of Dental Medicine Hokkaido University

• Ueda Yasuo

  Department Of Chemistry Faculty Of Science Osaka University:osaka Titanium Co. Ltd.

• Kuboki Yoshinori

  Department Of Biochemistry Hokkaido University Dental School

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