Chemotactic Response of Periodontal Ligament Cells toward rhBMP-2

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概要

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• Periodontal ligament cells(PDL cells)are responsible for the functions and maintenance of periodontal tissue. However, little is known about the regulatory mechanism for PDL cell activity. The aim of this study is to confirm whether PDL cells possess chemotactic activity toward recombinant human bone morphogenetic protein 2(rhBMP-2). Fresh, healthy PDL cells were obtained from the first and second premolars of orthodontic patients. It was found that chemotactic activity of rhBMP-2 for PDL cells started at 1-10 ng/ml of rhBMP-2, gradually increased in a dose-dependent manner, reached the highest value at 5 μg/ml, and then decreased at 10 μg/ml. On the other hand, PDL cells began to react with basic faibroblast growth factor(bFGF)at 10^<-2>ng/ml, reached the maximum reaction at 10 ng/ml. The high reactions continued until the concentration of 10^3 ng/ml and decreased thereafter. Overall reactions of PDL cells toward bFGF were much higher than those toward rhBMP-2. In contrast to the PDL cells, chemotactic response of osteoblast-like cells MC3T3-E1(E1 cells)toward rhBMP-2 and bFGF showed both in a typical bell-shaped manner, were attained maximal concentration of 100 and 10 ng/ml, respectively. The results indicate that PDL cells and E1 cells have their feasible cytokines, toward which they are induced chemotaxis and also have optimal concentrations of the cytokines for this reaction. The specificity in chemotactic reactions of these two cytokines for PDL cells and osteogenic E1 cells is important to note in therapeutic applications for periodontal regeneration.

• 硬組織再生生物学会の論文

著者

• SATO Noriyuki

  Department of Pathology (Section 1), Sapporo Medical University School of Medicine

• Sato Noriyuki

  Department Of Orthopedic Surgery Sapporo Medical University School Of Medicine

• Ueda Yasuo

  Dept. Of Oral Functional Science Graduate School Of Dental Medicine Hokkaido University

• Takita Hiroko

  Department Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Takita H

  Central Research Institute

• Sato Noriyuki

  Dept. Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Ueda Yasuo

  Department Of Oral Functional Science Hokkaido University Graduate School Of Dental Medicine

• Kuboki Y

  Hokkaido University

• Kuboki Yoshinori

  Emeritus Professor Hokkaido University

• Ohata Noboru

  Department of Prosthetic Dentistry II, School of Dentistry, Hokkaido University

• Ohata Noboru

  Department Of Oral Functional Science Hokkaido University Graduate School Of Dental Medicine

• Uno Keiji

  Dept. Of Oral Functional Science Graduate School Of Dental Medicine Hokkaido University

• Kuboki Yoshinori

  Dept. Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Kuboki Yoshinori

  Dept Biochem Sch Dentistry Hokkaido University

• Ohata Noboru

  Dept. Of Oral Functional Science Graduate School Of Dental Medicine Hokkaido University

• Ukegawa Tetsuya

  Dept. of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University

• UNO Keiji

  Department of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University

• UKEGAWA Tetsuya

  Department of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University

• TAKITA Hiroko

  Deepartment of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University

• MIZUNO Morimichi

  Deepartment of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University

• KUBOKI Yoshinori

  Deepartment of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University

• Takita Hiroko

  Dept Biochem Sch Dentistry Hokkaido University

• Ukegawa Tetsuya

  Dept. Of Oral Functional Science Graduate School Of Dental Medicine Hokkaido University

• Mizuno Morimichi

  Dept. Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Mizuno Morimichi

  Department Of Biochemistry Hokkaido University School Ofdentistry

• Ohata Noboru

  Department Of Oral Functional Science Graduate School Of Dental Medicine Hokkaido University

• Ueda Yasuo

  Department Of Chemistry Faculty Of Science Osaka University:osaka Titanium Co. Ltd.

• Sato Noriyuki

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

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