Chromatography of carbon nanotubes separated albumin from other serum proteins : a method for direct analysis of their interactions

スポンサーリンク

概要

• 論文の詳細を見る

• Chromatography technology was employed to clarify the mechanism of interaction between multi-wall carbon nanotubes (MWCNT) and proteins. A column (16×100 mm) was packed with purified MWCNT, and various proteins were eluted with phosphate buffered saline (PBS) with and without gradient systems. It was found that albumin in bovine serum was eluted immediately from the column without any adsorption to MWCNT. Conversely, the non-albumin proteins, including a protein of 85 kDa molecular mass and a group of proteins with molecular masses higher than 115 kDa, exhibited considerably high affinity towards MWCNT. A sample of pure bovine serum albumin was also eluted immediately from the column, while lysozyme did not elute as a peak with PBS, but eluted with 0.6 M NaCl. Fundamentally, carbon nanotubes are devoid of any electrical charge. Therefore, other forces including the hydrogen bonds, hydrophilic interactions, and van der Waals forces were most probably responsible for the differential elution behaviors. In conclusion, this chromatographic method provided a simple and direct analysis of the interactions between carbon nanotubes and the various proteins.

• 2010-07-01

著者

• Watari Fumio

  Dep. Of Biomedical Dental Materials And Engineering Graduate School Of Dental Medicine Hokkaido Univ

• Watari Fumio

  Hokkaido Univ. Sapporo Jpn

• Watari Fumio

  北海道大学 歯学研究科口腔健康科学

• Watari Fumio

  北海道大学 エネルギー変換マテリアル研究センター

• Akasaka Tsukasa

  北海道大学 歯学研究科生体理工学

• Akasaka Tsukasa

  Department Of Biomedical Dental Materials And Engineering Graduate School Of Dental Medicine Hokkaid

• Watari F

  Department Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Watari Fumio

  Department Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Akasaka Tsukasa

  Department Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Kawakami Toshiyuki

  Department Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Uo Motohiro

  Department Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Uo M

  Hokkaido Univ. Sapporo Jpn

• Watari F

  Hokkaido Univ. Sapporo Jpn

• Watari Fumio

  北海道大学 歯研究 口腔機能

• Uo Motohiro

  Department Of Biomedical Materials And Engineering Graduate School Of Dentistry Hokkaido University

• ABE Shigeaki

  Department of Biomedical Materials and Engineering, Graduate School of Dental Medicine, Hokkaido Uni

• Watari Fumio

  Department Of Applied Science Faculty Of Engineering

• Lee Min-ho

  Department Of Dental Biomaterials School Of Dentistry And Institute Of Oral Bioscience Brain Korea 2

• Uo Motohiro

  Dental Materials And Engineering Department Of Oral Health Science Graduate School Of Dental Medicin

• Takita Hiroko

  Department Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Takita Hiroko

  Department Of Biochemistry Hokkaido University Dental School

• Kaku T

  Department Of Oral Pathology School Of Dentistry Health Sciences University Of Hokkaido

• Kaku Tohru

  Department Of Oral Pathology Hokkaido Health Sciences University Of Hokkaido

• Kuboki Yoshinori

  Emeritus Professor, Hokkaido University , Koken bioscience institute

• FUJISAWA Ryuichi

  Department of Microbiology, Kinki University School of Medicine

• Kuboki Yoshinori

  Emeritus Professor Hokkaido University

• KOSHIKAWA Takamitu

  Department of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University

• SAMMONS Rachel

  Department of Biomaterials, School of Dentistry, University of Birmingham

• Fujisawa Ryuichi

  Department Of Biochemistry School Of Dentistry Hokkaido University

• Fujisawa Ryuichi

  Departments Of Bacterioloby Kinki University School Of Medicine

• Fujisawa Ryuichi

  Department Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Kuboki Yoshinori

  Dept. Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Kuboki Yoshinori

  Dept Biochem Sch Dentistry Hokkaido University

• Akazawa Toshiyuki

  Department Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Abe Shigeaki

  Department Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Sammons Rachel

  Department Of Biomaterials School Of Dentistry University Of Birmingham

• Hirai T

  Department Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Fujisawa R

  Hokkaido Univ. Sapporo Jpn

• Kaku Tohru

  Deparment Of Oral Pathology School Of Dentistry Health Sciences University Of Hokkaido

• Uo Motohiro

  Department Of Biomedical Materials And Engineering Graduate School Of Dental Medicine Hokkaido Unive

• Akazawa Toshiyuki

  Industrial Research Institute Industrial Technology Research Development Hokkaido Research Organizat

• Akazawa Toshiyuki

  Department Of Materials Technology Hokkaido Industrial Research Institute

• UO Motohiro

  Department of Advanced Biomaterials, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University

• ABE Shigeaki

  Department of Biomaterials and Bioengineering, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan

関連論文

• Strong adhesion of Saos-2 cells to multi-walled carbon nanotubes
• Characterization of microstructure and bio-absorption of the hydroxyapatite ceramics modified by a partial dissolution–precipitation technique using supersonic treatment
• Bone induction of human tooth and bone crushed by newly developed automatic mill
• Development of a 3D collagen scaffold coated with multiwalled carbon nanotubes
• Multiwalled carbon nanotube coating on titanium
• Multiwalled Carbon Nanotube Monoliths prepared by Spark Plasma Sintering (SPS) and their Mechanical Properties
• 3D collagen scaffolds coated with multiwalled carbon nanotubes : Initial cell attachment to internal surface
• A-9 Chitosan/BSA micropatterns on Titanium Surfaces by Functionization and Soft Lithography
• Expression pattern of p63 in oral epithelial lesions and submucous fibrosis associated with betel-quid chewing in Sri Lanka
• Increased expression of β-defensin-2 and -3 during the development of autoimmune sialoadenitis in MRL/Ipr mice
• Effect of defensin peptides on eukaryotic cells : primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines
• Atypical carcinoid (neuroendocrine carcinoma) of the gingiva : Counterpart of a laryngeal tumor
• An Immunohistochemical Study of the Localization of Inducible Nitric Oxide Synthase (iNOS) and Heat Shock Protein (HSP) in Pleomorphic Adenoma
• Localization of human β-defensin 3 mRNA in normal oral epithelium, leukoplakia, and lichen planus : an in situ hybridization study
• Immunohistochemical localization of amelogenin in human odontogenic tumors, using a polyclonal antibody against bovine amelogenin
• Primary Intraosseous Carcinoma : A review of Japanese cases
• Basaloid-squamous cell carcinoma of the oral mucosa : Report of two cases and study of the proliferative activity
• Localization of p21/waf-1/cip-1 mRNA at the Terminal Differentiated Stage in Rat Dental Tissues
• BCL-2 expression in epithelial rest of Malassez, both in vivo and in vitro
• Apoptosis in the Reduced Enamel Epithelium just after Tooth Emergence in Rats : In Situ Labeling of Nuclear DNA Fragmentation and Electron Microscopic Studies
• Salivary Duet Carcinoma : Report of a case, immunohistochemical studies and a survey of Japanese cases
• Polymorphous Low Grade Adenocarcinoma of Minor Salivary Gland Origin in the Oral Floor : Report of a case with an immunohistochemical study and an analysis of proliferative activity
• Apoptotic cell death at tooth eruption in rat molar observed by in situ labeling of nuclear DNA fragmentation
• XAFS Analysis of Ti and Ni Dissolution from Pure Ti, Ni-Ti Alloy, and SUS304 in Soft Tissues
• Chromatography of carbon nanotubes separated albumin from other serum proteins : a method for direct analysis of their interactions
• Histological Patterns of Salivary Gland Tumor Observed by Confocal Laser Scanning Microscope
• Immunohistochemical localization of large chondroitin sulfate proteoglycan in odontogenic tumor
• An Immunohistochemical Study of the Localization of Biglycan, Decorin and Large Chondroitin-sulphate Proteoglycan in Pleomorphic Adenoma
• AFM and SEM Observation of Morphological Changes of Human Dentin Surface by Treatment of Acidic Agents
• Effects of Extracellular Calcium on the Proliferation, Differentiation and Gene Expressions of Bone Morphogenetic Proteins-2 and -4 in Human Gingiva-Derived Fibroblasts
• X-ray Absorption Fine Structure Studies on the Local Structures of Ni Impurities in a Carbon Nanotube
• Development of a multiwalled carbon nanotube coated collagen dish
• Behavior of in vitro, in vivo and internal motion of micro/nano particles of titanium, titanium oxides and others( Ceramics for Biomedical Applications in Asian Countries)
• X-ray Absorption Fine Structure (XAFS) Analysis of　Titanium-implanted Soft Tissue
• Analysis of Titanium Dental Implants Surrounding Soft Tissue Using X-ray Absorption Fine Structure (XAFS) Analysis
• Effects of Particle Size on Cell Function and Morphology in Titanium and Nickel
• Human acid-insoluble dentin with BMP-2 accelerates bone induction in subcutaneous and intramuscular tissues
• Regenerative Medicine of Bone and Teeth : with special references to biological principles, problems and their indicators
• Properties of osteoinduction and BMP-2 release in β-TCP from spongy bone(Oral Session,The 16th Annual Meeting of the Society for Hard Tissue Regenerative Biology in Matsumoto Dental University)
• Properties of osteoinduction and BMP-2 release in functionally graded HAp(15thAnnual Meeting of the Society for Hard Tissue Regenerative Biology,Session 2)
• Induction of bone and cartilage by functionally graded-HAp/BMP-2 on rat skull periosteum(15thAnnual Meeting of the Society for Hard Tissue Regenerative Biology,Session 2)
• Materials Design and Osteoinduction Characteristics of Biomimetic and Functionally Graded Apatites(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
• Bone induction by a newly developed, biomimetic HA with calcium phosphate-garadeint and rhBMP-2
• Application of flake shaped glass (Glass Flake®) filler for dental composite resin
• Infrared Absorption Spectra of Molecular Complexes of Pyridine. Part V : Molecular Complexes with Cu-, Co-, Ni- and Mn-Dihalides
• Fabrication of Titanium Nitride/Apatite Functionally Graded Implants by Spark Plasma Sintering
• High-resolution electron microscopy of multi-wall carbon nanotubes in the subcutaneous tissue of rats
• The Purity and Thermal Stability in Air of Metal-Encapsulating Carbon Nanocapsules (MECNCs)
• Carbon Nanotubes as Scaffolds for Cell Culture and Effect on Cellular Functions
• Strikingly Extended Morphology of Cells Grown on Carbon Nanotubes
• Apatite formation on carbon nanotubes
• Selenium Distribution in Human Soft Tissue Determined by Using X-ray Scanning Analytical Microscope and X-ray Absorption Fine Structure Analysis
• Novel Bulk Carbon Nanotube Materials for Implant by Spark Plasma Sintering
• Rare earth oxide containing fluorescent glass filler for composite resin
• Single-nucleotide Polymorphism (SNP) in β-Defensin 2 in a Japanese Population and an Effect of - 1029 SNP on Promoter Activity
• Upregulated expression of human β defensin-1 and-3 mRNA during differentiation of keratinocyte immortalized cell lines, HaCaT and PHK16-Ob
• Large-Scale Production of Ba^-Alginate-Coated Vesicles of Carbon Nanofibers for DNA-Interactive Pollutant Elimination
• Expression of inflammatory cytokines and beta-defensin 1 mRNAs in porcine epithelial rests of Malassez in vitro
• Presence of Human Beta-Defensin 2 Peptide in Keratinization in Salivary Gland Tumor
• Nano-architecture on Carbon Nanotube Surface by Biomimetic Coating
• Fabrication of a Functionally Graded Dental Composite Resin Post and Core by Laser Lithography and Finite Element Analysis of its Stress Relaxation Effect on Tooth Root
• Firing Shrinkage of Porcelain-resin Composites Prepared by Laser Lithography
• Mechanical Properties of Surface Nitrided Titanium for Abrasion Resistant Implant Materials
• Surface Properties and Biocompatibility of Nitrided Titanium for Abrasion Resistant Implant Materials
• Effects of mechanical stress on the eryression of type XII collagen mRNA in human periodontal ligament cells
• Imaging and Non-Contact Profile Analysis of Nd : YAG Laser-Irradiated Teeth by Scanning Electron Microscopy and Confocal Laser Scanning Microscopy
• Temperature-dependence of the Mechanical Properties of FRP Orthodontic Wire
• Development of New Dental Materials by Combining Substances with Varying Properties
• Expression of claudin-4 and -7 in porcine gingival junctional epithelium
• Synthesis of DNA - Carbohydrate Conjugate via Diazocoupling ; A New Class of Modified DNA with Enhanced Stability and Lectin - Recognition Ability
• In-situ etching observation of human teeth in acid agent by atomic force microscopy
• XAFS Analysis of the Bronchoalveolar Lavage Fluid of a Tungsten Carbide Pneumoconiosis Patient
• Inflammatory Exudates Modulate the Function and Apoptosis of Neutrophils
• Distortion of Laser Welded Titanium Plates
• Laser Welding of Titanium and Dental Precious Alloys
• Slip Casting of Titanium Powder for Dental Prosthetic Appliances
• Non-Contact Surface Morphology Analysis of CO_2 Laser-Irradiated Teeth by Scanning Electron Microscope and Confocal Laser Scanning Microscope
• Fluorescent Properties of Porcelain-Restored Teeth and Their Discrimination
• Discrimination between Composite Resin and Teeth using Fluorescence Properties
• Effect of surface condition of dental zirconia ceramic (Denzir) on bonding
• Surface analysis of dental amalgams by X-ray photoelectron spectroscopy and X-ray diffraction
• Toxicity evaluations of various carbon nanomaterials
• Phase transformation of zirconia ceramics by hydrothermal degradation
• In vivo Evaluation of a Novel Chitosan/HAp Composite Biomaterial as a Carrier of rhBMP-2
• Discrimination of Albumin and Lysozyme-Adsorption Characteristics on Two Differently-Originated Apatites
• A Newly Designed Adsorbent Prepared from Hydroxyapatite Originating from Cattle-Bones for Chromatographic Separation of Albumin and Lysozyme
• High Resolution Adsorbent of Liquid Chromatography for Protein Analysis : Cattle Bone-Originated Hydroxyapatites Characterized by Heating
• Toxicity evaluations of various carbon nanomaterials
• Thin films of single-walled carbon nanotubes promote human osteoblastic cells (Saos-2) proliferation in low serum concentrations
• Capture of bacteria by flexible carbon nanotubes
• Carbon nanotube-coated silicone as a flexible and electrically conductive biomedical material
• The bactericidal effect of carbon nanotube/agar composites irradiated with near-infrared light on Streptococcus mutans
• Modification of the dentin surface by using carbon nanotubes.
• Adhesion of human osteoblast-like cells (Saos-2) to carbon nanotube sheets.
• Effects of Functionally Graded Hydroxyapatite for Large Mandibular Defects in Adult Rabbits
• An Evaluation of Fluorescent Orthodontic Adhesives Containing Eu-doped ZnO at Room Temperature
• Introduction of Rare-Earth-Element- Containing ZnO Nanoparticles into Orthodontic Adhesives
• Effect of Pretreatments to Carbon Nanotubes on Protein Adsorption
• Carbonate Ion Induced Calcification Using Biomimetic Enzymes with Active Reaction Center of Carbonc Anhydrase
• Evaluation of Adhesion between Material and Epithelium using a Three-dimensional Human Epidermal Model

もっと見る 閉じる

スポンサーリンク