Strong adhesion of Saos-2 cells to multi-walled carbon nanotubes
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概要
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In recent years, carbon nanotubes (CNTs) have been considered potential biomedical materials because of their unique character. The aim of this study was to investigate the response of a human osteoblast-like cell line - Saos-2 - on single-walled CNTs (SWCNTs) and multi-walled CNTs (MWCNTs). The surface of a culture dish was coated with CNTs, and Saos-2 cells were cultured for three days. Cell morphology, viability, alkaline phosphatase (ALP) activity, adhesion, and vinculin expression were evaluated. The result showed high cell viability and strong adhesion to MWCNTs. Saos-2 cultured on MWCNTs exhibited vinculin expression throughout the cell body. while the cells attached to SWCNTs and glass were mostly limited to their periphery. Our results suggest that CNT coatings promote cell activity and adhesiveness. These findings indicate that MWCNTs could be used as surface coating materials to promote cell adhesion.
- 2010-10-15
著者
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Watari Fumio
Dep. Of Biomedical Dental Materials And Engineering Graduate School Of Dental Medicine Hokkaido Univ
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Watari Fumio
Hokkaido Univ. Sapporo Jpn
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Watari Fumio
北海道大学 歯学研究科口腔健康科学
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Watari Fumio
北海道大学 エネルギー変換マテリアル研究センター
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Akasaka Tsukasa
北海道大学 歯学研究科生体理工学
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Akasaka Tsukasa
Department Of Biomedical Dental Materials And Engineering Graduate School Of Dental Medicine Hokkaid
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Akasaka Tsukasa
Department Of Molecular Design And Department Of Biotechnology Graduate School Of Engineering Nagoya
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Watari F
Department Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University
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Akasaka Tsukasa
Department Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University
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Watari F
Hokkaido Univ. Sapporo Jpn
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Watari Fumio
北海道大学 歯研究 口腔機能
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Totsuka Yasunori
Oral And Maxillofacial Surgery Graduate School Of Dental Medicine Hokkaido University
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Akazawa Toshiyuki
Department Of Materials Technology Hokkaido Industrial Research Institute
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