Human acid-insoluble dentin with BMP-2 accelerates bone induction in subcutaneous and intramuscular tissues
スポンサーリンク
概要
- 論文の詳細を見る
Dentin matrix is well known as the most insoluble collagen in human body. We already confirmed the osteoinductive property by granules of human demineralized dentin matrix (DDM) histologically. Human DDM granules and human demineralized root-dentin (DRD) mass were prepared from adult vital teeth. The hard tissue induction by DDM alone or DRD alone was estimated at 4 weeks after implantation. The DDM alone induced bone and cartilage independently. In addition, the bioassay by recombinant human BMP-2 (5.0 µg)/DDM or BMP-2 (5.0 µg)/DRD was estimated in mouse subcutaneous or intramuscular tissues, respectively. Histological examination showed that the BMP-2/DDM induced bone and marrow, and the DDM granules were partially absorbed by new bone. The morphometric analysis demonstrated that the BMP-2/DDM showed 36.3% in the volume of bone and marrow, while the DDM alone showed 1.3% at 4 weeks. Moreover, the BMP-2/DRD also induced active bone formation on the root surface and in the pulp cavity. These results indicate that BMP-2 significantly accelerated bone formation in decalcified dentin implants. Human recycled DDM and DRD might be effective materials as osteoinductive collagenous carriers of BMP-2 for bone engineering.
- 公益社団法人 日本セラミックス協会の論文
著者
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Murata Masaru
Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido
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Akazawa Toshiyuki
Materials Technology, Hokkaido Industrial Research Institute
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ARISUE Makoto
Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido
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Kawakami Toshiyuki
Department Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University
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Kusano Kaoru
Oral And Maxillofacial Surgery School Of Dentistry Health Sciences Univ. Of Hokkaido
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KAWAI Tatsushi
Dental Material Science, School of Dentistry, Aichi-Gakuin University
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ITO Katsutoshi
Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido
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Kaku T
Department Of Oral Pathology School Of Dentistry Health Sciences University Of Hokkaido
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Kaku Tohru
Department Of Oral Pathology Hokkaido Health Sciences University Of Hokkaido
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Kawakami Toshiyuki
Inst. For Oral Sci. & High-tech Center Matsumoto Dental Univ.
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Murata Masaru
Department Of Oral Maxillofacial Surgery School Of Dentistry Health Sciences University Of Hokkaido
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Kawakami Toshiyuki
Institute for Oral Science & High-Tech Center, Matsumoto Dental University
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TAZAKI Junichi
Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido
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Murata Masaru
Oral And Maxillofacial Surgery School Of Dentistry Health Sciences University Of Hokkaido
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Arisue Makoto
Oral And Maxillofacial Surgery School Of Dentistry Health Sciences Univ. Of Hokkaido
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Kawai Tatsushi
Department Of Dental Material Science School Of Dentistry Aichi-gakuin University
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Kawai Tatsushi
Dental Material Science School Of Dentistry Aichi-gakuin University
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Murata Masaru
2nd Department Of Oral Surgery School Of Dentistry Health Sciences University Of Hokkaido
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Murata Masaru
Department Of Oral And Maxillofacial Surgery School Of Dentistry Health Sciences University Of Hokka
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Kaku Tohru
Deparment Of Oral Pathology School Of Dentistry Health Sciences University Of Hokkaido
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Murata Masaru
Department Of Oral And Maxillofacial Surgery School Of Dentistry Health Sciences University Of Hokka
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Murata Masaru
Oral And Maxillofacial Surgery Human Biology And Pathopysiology School Of Dentistry Health Sciences University Of Hokkaido
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Arisue Makoto
Oral And Maxillofacial Surgery Human Biology And Pathopysiology School Of Dentistry Health Sciences University Of Hokkaido
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KAWAKAMI Toshiyuki
Institute for Oral Science & High-Tech Center, Matsumoto Dental University
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