Bone Healing in Large Mandibular Defects Without Periosteum in Adult Rabbits : A New Application of Collagenous Sponge for Bone Regeneration

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The efficacy of different filling materials; namely, non-absorbable byhydroxyapatite(HA)and absorbable collagen, for bone healing was evaluated in critical size mandibular bone defects without periosteum, focusing on the healing process between the cortical cut-ends. Thirty-six Japanese white rabbits were operated to create a single critical size cortical bone defect(12×9×3.5mm)without periosteum in the right mandible. The bone defects were left empty(unfilled). or filled with 200 mg of HA particles alone, or 100 mg of HA particles and a collagenous sponge sheet(HA was filled up to the inner cortical line between cut-ends in the defect and the remaining space was covered with collagenous sponge). At 7, 14 and 21 days after operation, the mandibles were removed and coronal sections were stained with hematoxylin-eosin and Elastica van Gieson for fistological observation and morphometric analysis. The collagen/HA-filled defects achieved complete formation of a bony bridge between the cortical cut-ends by day 21, which was lined with active osteoblasts and contained blood vessels; whereas HA-filled defects exhibited incomplete bony bridging, and unfilled defects was interposed by soft tissues. Histomorphometric analysis of an area(5 mm in length, 1 mm in width)corresponding to a central region between two cut-ends on day 21 demonstrated that the bone tissue occupied 75% of the volume in the collagen/HA group, compared with 2.4% in the unfilled group. The collagenous sponge in the collagen/HA group decreased in volume sequentially. Our results indicate that the collagenous matrix provides a substratum for the proliferation and differentiation of mesenchymal cells and promotes bony bridging across the cortical cut-ends. The effectiveness of the collagenous sponge to promote healing of bone defects without periosteum may be through acting as a bone substitute that is subsequently absorbed and replaced by new bone.
硬組織再生生物学会の論文

Bone Healing in Large Mandibular Defects Without Periosteum in Adult Rabbits : A New Application of Collagenous Sponge for Bone Regeneration

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