Gene Expression of Bone Matrix Protein mRNA during BMP Induced Chondrogenesis and Osteogenesis by in situ Hybridization
スポンサーリンク
概要
- 論文の詳細を見る
A sensitive in situ hybridization method with high resolution has been developed usin digoxigenin-II VTP labeled cRNA as a probe. The system was applied to a heterotopic induced bone with crude BMP. The localization of mRNA of the bone matrix proteins, osteopontin (OSP) and osteocalcin (OSC), was examined in the BMP-induced cartilage and bone in comparison with the endochondral ossification. In the heterotopic bone, OSP mRNA were detected in the osteoblast, young osteocyte and chondrocyte-like cells. In fatal endochondral ossification OSP mRNA was detected in the hypertrophic chondrocyte, osteoblast and young osteocyte, while OSC mRNA was detected in the osteoblast and young osteocyte, but hypertrophic chondrocyte was not detected. These results suggest that the chondrocyte-like cells in the BMP induced bone demonstrate the ambifunctions of the chondrocyte and osteoblast. Our results indicate that in situ hybridization immunohistochemistry is useful in identifying the cell phenotypes and their function.
- 硬組織再生生物学会の論文
著者
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Nagatsuka Hitoshi
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry & Pharmaceutical S
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Nagai Noriyuki
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry & Pharmaceutical S
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Nakano Keisuke
Department of Oral Pathology and Medicine, Graduate School of Medicine and Dentistry, Okayama Univer
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MURATA Masaru
Department of Oral Maxillo-facial Surgery, School of Dentistry, Health Sciences University of Hokkai
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Inoue Masahisa
Laboratories For Structure And Function Research Faculty Of Pharmaceutical Sciences Tokushima Bunri
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Inoue Masahisa
徳島文理大学 薬学部機能形態学
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Tsujigiwa Hidetsugu
Department Of Oral Pathology And Medicine Graduate School Of Medicine & Dentistry Okayama Univer
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Nakano Keisuke
Hard Tissue Pathology Unit Matsumoto Dental University Graduate School Of Oral Medicine
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Murata M
Okayama Univ. Okayama Jpn
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Takagi Tohru
Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A
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Takagi Tohru
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Konouchi Hironobu
Department of Oral pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical
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Qin Chun-lin
Section Of Biochemistry The University Of Texas-houston Health Science Center Dental Branch
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Akagi Takumi
Dept. of Oral Pathology, Okayama Univ. Dental School
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Inoue Masahisa
Department of Oral Pathology, School of Dentistry, Okayama University
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QIN Chun-Lin
Department of Oral Pathology, School of Dentistry, Okayama University
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AKAGI Takumi
Department of Oral Pathology, School, of Dentistry, Okayama University
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CHIGONO Yoshiho
First Department of Oral Surgery, Faculty of Medicine, Teikyo University
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ISIWARI Yuzo
Department of Oral Pathology, Okayama University Dental School
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CHIGONO Yoshiho
Department of Oral Surgery, Faculty of Medicine, Teikyo University
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Konouchi Hironobu
Japan Institute For Advanced Dentistry:okayama University Graduate School Of Medicine Dentistry And
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Qin Chun-lin
Department Of Oral Pathology School Of Dentistry Okayama University
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Nagatsuka Hitoshi
Department Of Oral Pathology And Medicine Graduate School Of Medicine And Dentistry Okayama Universi
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Konouchi Hironobu
Department Of Oral And Maxillofacial Radiology Okayama University Graduate School Of Medicine And De
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Konouchi Hironobu
Dept. Of Oral Pathology Okayama Univ. Dental School.
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Nagai Noriyuki
Department Of Oral Pathology And Medicine Dental School Okayama University
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Murata Masaru
Department Of Oral Pathology School Of Dentistry Okayama University
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Murata Masaru
Department Of Oral Maxillofacial Surgery School Of Dentistry Health Sciences University Of Hokkaido
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Akagi Takumi
Dept. Of Oral Pathology Okayama Univ. Dental School
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Akagi Takumi
Department Of Oral Pathology School Of Dentistry University Of Okayama
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Murata Masaru
Department Of Architecture And Building Engineering School Of Engineering
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Nakano Keisuke
Department Of Information Engieering Niigata University
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Ishiwari Yuzo
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Chigono Yoshiho
First Department Of Oral Surgery Faculty Of Medicine Teikyo University
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Chigono Yoshiho
Department Of Dental Oral Surgery School Of Medicine Teikyo University
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Inoue Masahisa
Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A
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Kou Hirokuni
Japan Institute For Advanced Dentistry:okayama University Graduate School Of Medicine Dentistry And
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Ishiwari Yuzo
Department Of Oral Pathology Okayama University Dental School
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Takagi Tohru
Department Of Biochemistry Faculty Of Dentistry Tokyo Medical And Dental University
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Tujigiwa Hidetugu
Department Of Oral Pathology And Medicine Graduate School Of Medicine And Dentistry. Okayama Univers
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Murata Masaru
Department Of Oral And Maxillofacial Surgery School Of Dentistry Health Sciences University Of Hokka
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Takagi Tohru
Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A
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Tujigiwa Hidetugu
Dept. Of Oral Pathology Okayama Univ. Dental School.
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Takagi Tohru
Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni
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Takagi Tohru
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Murata Masaru
Department Of Oral And Maxillofacial Surgery School Of Dentistry Health Sciences University Of Hokka
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Kou Hirokuni
Japan Institute For Advanced Dentistry:okayama University Graduate School Of Medicine Dentistry And
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Inoue Masahisa
Department Of Biomaterials And Biomimetics College Of Dentistry New York University
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Konouchi Hironobu
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Takagi Tohru
Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni
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Takagi Tohru
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Inoue Masahisa
Laboratories For Structure And Function Research Faculty Of Pharmaceutical Sciences Tokushima Bunri
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Nakano Keisuke
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Tsujigiwa Hidetsugu
Department Of Oral Pathology School Of Dentistry Okayama University
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Akagi Takumi
Department Of Oral Pathology Okayama University Dental School
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