Influence of Three-Dimensional Scaffold on Bone Induction by KUSA/A1 Cells
スポンサーリンク
概要
- 論文の詳細を見る
Recently, atelocollagen has been used in tissue engineering as one of the most useful scaffolds for bone formation. However, the optimal scaffold-structure for bone engineering has not been determined. Hence, we compared the honeycomb porous structure (HPS) and the interconnected porous structure (IPS) with or without KUSA/A1 cells in vivo. KUSA/A1 cells alone, HPS alone, IPS alone, KUSA/A1-HPS and KUSA/A1-IPS were implanted in the subcutaneous pockets of 4-weeks-old male SCID mice. The implants were examined radiologically and histologically 1, 2, 4 and 8 weeks after implantation. Radiologically, small radiopaque islands of new bone were observed in the implant of KUSA/A1 cells alone. No radiopacity was observed with both scaffols alone. In the KUSA/A1-HPS implant, the scaffold was partially filled with radiopaque areas. In contrast, in the KUSA/A1-IPS implant, the whole scaffold was filled with a well-defined radiopaque area. Histologically, KUSA/A1 cells alone induced only small nests of new bone. HPS alone produced inflammatory reaction. A large bone was observed in implants of KUSA/Al cells combined with both scaffolds, but the scaffold was completely filled with new bone without inflammatory reaction within a shorter period in the KUSA/A1-IPS compared to KUSA/A1-HPS implant. Our results indicated that a cotton structured scaffold plays an important role in carrying the cells, providing the precise size, shape and comfortable environment. These results have potential impact to enhance existing therapeutic strategies.
- 硬組織再生生物学会の論文
著者
-
Nagatsuka Hitoshi
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry & Pharmaceutical S
-
Tamamura Ryo
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry & Pharmaceutical S
-
Nagai Noriyuki
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry & Pharmaceutical S
-
Rodriguez Andrea
Department of Oral Pathology and Medicine, Graduate School of Medicine and Dentistry, Okayama Univer
-
Hidetsugu Tsujigiwa
Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni
-
Tsujigiwa Hidetsugu
徳島文理大学 薬学部機能形態学
-
Tsujigiwa Hidetsugu
Department Of Oral Pathology And Medicine Graduate School Of Medicine & Dentistry Okayama Univer
-
L Missana
Tucuman University
-
Missana Liliana
Department Of Oral Pathology Dental School Tucuman University
-
Andrea Rodriguez
Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A
-
Rodriguez Andrea
Department Of Oral Pathology Dental School Tucuman University:department Of Oral Pathology And Medic
-
Gunduz Mehmet
Department Of Otolaryngology Head And Neck Surgery Wakayama Medical University
-
Borkosky Silvia
Department of Oral Pathology, Tucuman National University, School of Dentistry
-
Han Phuu
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical
-
Nagatsuka Hitoshi
Department Of Oral Pathology And Medicine Graduate School Of Medicine And Dentistry Okayama Universi
-
Nagai Noriyuki
Department Of Oral Pathology And Medicine Dental School Okayama University
-
Han Phuu
Orofacial Pain And Oral Medicine Center Division Of Diagnostic Sciences School Of Dentistry Universi
-
Tamamura Ryo
Department Of Oral Pathology And Medicine Dentistry And Pharmaceutical Sciences Okayama University
-
Tamamura Ryo
Deparment Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical Sc
-
Borkosky Silvia
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
-
Tujigiwa Hidetugu
Department Of Oral Pathology And Medicine Graduate School Of Medicine And Dentistry Okayama Universi
-
Tsujigiwa Hidetsugu
Department Of Oral Pathology And Medicine Graduate School Of Medicine And Dentistry. Okayama Univers
-
Gunduz Mehmet
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
-
Rodriguez Andrea
Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni
-
Rodriguez Andrea
Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A
-
Tujigiwa Hidetugu
Department Of Oral Pathology And Medicine Graduate School Of Medicine And Dentistry. Okayama Univers
-
Tamamura Ryo
Department Of Oral Pathology And Medicine Graduate School Of Medicine And Dentistry Okayama Universi
-
Tujigiwa Hidetugu
Dept. Of Oral Pathology Okayama Univ. Dental School.
-
Nagai Noriyuki
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
-
Rodrigez Andrea
Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A
-
Tsujigiwa Hidetsugu
Department Of Oral Pathology School Of Dentistry Okayama University
-
Rodriguez Andrea
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
-
Rodriguez Andrea
Department Of Oral Pathology And Medicine Graduate School Of Medicine And Dentistry Okayama University
関連論文
- Human Papillomaviruses Infection In Oral Squamous Cell Carcinoma And Oral Pre-cancer Lesions In Taiwan(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
- Wnt5a Overexpression in Thick Primary Oral Mucosal Melanomas : Implications for its Role in Tumor Progression
- Fluoride Supplement Affects Bone Mineralization in Young Rats
- Gene Expression of Matrix Proteins in Cbfal-Knockout Mice(Asian Science Seminar/I-1, The 3^ Asian Science Seminar)
- Vascular Channel of Oral Malignant Melanoma with Metastasis
- Gene expression of matrix proteins in Cbfal- knockout mice(2nd ASIAN SCIENCE SEMINAR IN OKAYAMA 2003)
- Bone Morphogenetic Protein (BMP) Stimulates Cytological and Functional Differentiation of Preodontoblasts in vitro
- Immunohistochemical Characteristics of Odontogenic Neoplasms and Their Physiological Counterparts
- The Study of Palatal Cell Proliferation and Apoptosis in Retinoic Acid Induced Mouse Cleft Palate Varied with Different Developmental Stage
- Role of Heparanase in the Release of Heparan Sulphate Binding Growth Factors in Odontogenic Tumors
- Sequential expression of Notch1, HES5, Jagged2, and Math1 in molar tooth germ of mouse(5th Asian Science Seminar,Session 1)
- Differential Distribution of Type IV Collagen α1 to α6 Chains Suggests Distinct Molecular Interaction between the Epithelial and Mesenchymal Components of Benign Odontogenic Tumors
- Frequent Deletion of BRG1 Locus at 19p13 Predicts Recurrence and Previous Cancer History in Oral Squamous Cell Carcinomas
- Histopathological Study of Matrix Mineralization by Osteoblastic-like and Odontoblastic-like Cells in Diffusion Chamber
- Type I, II and X Collagen Gene Expression Pattern in Bone Formation Using rh-BMP2(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
- Localization of type IV collagen alpha chains in tooth germ development(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
- Aging and ectopic bone formation induced by partially purified bone morphogenetic protein
- Aging and Ectopic Bone Formation Induced by Partially Purified Bone Morphogenetic Protein : Blood Vessel Ingrowth and Localization of Type I Collagen and Osteocalcin Assessed by Immunohistochemistry
- Histopathological and Immunohistochemical Study of Heterotopic Chondro-Osseous Tissue Formation Induced by S-200 BMP
- Immunohistochemical Study of Heterotopic Cartilage-Bone Formation Induced by BMP-FGM
- Immunohistochemical Study of Cytokeratin Patterns in Follicular and Plexiform Ameloblastoma
- Gene Expression of Bone Matrix Protein mRNA during BMP Induced Chondrogenesis and Osteogenesis by in situ Hybridization
- The Effect of Enzymatic Treatment on Immunohistochemical Staining of Collagens in Mandibular Condyle of Rats
- Histopathological Studies of Heterotopic Bone Formations Induced by BMP Carrier Composites
- Wingless-type Protein-1 (Wnt-1) Expression in Primary Conventional and Unicystic Ameloblastomas and Their Recurrent Tumors
- Notch signaling in mandibular condylar cartilage development(Poster Session,The 16th Annual Meeting of the Society for Hard Tissue Regenerative Biology in Matsumoto Dental University)
- Notch signaling in malignant tranceforming of odontogenic tumor(Oral Session,The 16th Annual Meeting of the Society for Hard Tissue Regenerative Biology in Matsumoto Dental University)
- Origin of Osteoblasts Involved in the Mechanism of Ectopic Bone Formation Induced by KUSA/A1 Cells with Honeycomb Carrier
- Runx2 expression in mandibular condylar cartilage development(15thAnnual Meeting of the Society for Hard Tissue Regenerative Biology,Session 2)
- Gene Expression of Matrix Proteins during Tooth Germ Development in Cbfa1 Knockout Mice(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
- Gene Expression of Matrix Proteins in Cbfa1-Knockout Mice
- Effects of Immobilized rhBMP-2/atelocollagen in vivo and in vitro
- An investigation of the ability to differentiate to dental tissue cells of the bone marrow derived mesenchymal stem cells(Oral Session,The 16th Annual Meeting of the Society for Hard Tissue Regenerative Biology in Matsumoto Dental University)
- Biological analysis of a candidate stem cell-KUSA/A1 cell-for bone tissue engineering(Oral Session,The 16th Annual Meeting of the Society for Hard Tissue Regenerative Biology in Matsumoto Dental University)
- Evaluation of CaTiO_3-C in vitro study(Oral Session,The 16th Annual Meeting of the Society for Hard Tissue Regenerative Biology in Matsumoto Dental University)
- A basic research of CaTiO_3-C as a coating material for implants(Oral Session,The 16th Annual Meeting of the Society for Hard Tissue Regenerative Biology in Matsumoto Dental University)
- A Three-dimensional Finite Element Analysis for the Biomechanical Characteristics of Orthodontic Anchorage Micro-implant
- Improvement of Adhesion and Spreading of Human Gingival Fibroblasts to RGD-grafted Titanium Surface
- Expression of WISP-1 (ccn4), WISP-2 (ccn5) and WISP-3 (ccn6) in Rheumatoid Arthritic Synovium Evaluated by DNA Microarrays
- Patients with localized primary non-tonsillar oral diffuse large B-cell lymphoma exhibit favorable prognosis despite a non-germinal center B-cell-like phenotype
- Expression and Clinical Significance of Matrix Metalloproteinase-2 and Its Inhibitor TIMP-2 in Oral Squamous Cell Carcinoma
- Development of CaTiO_3-C coating material for dental implant(5th Asian Science Seminar,Session 1)
- 14. Distribution pattern of αl(IV)- α6(IV)chains of type IV collagen in tooth germ morphogenesis(8th Annual Meeting of the Society of Hard Tissue Biology July 24, 1999 in Asahikawa)
- 13. Bone Augmentation by BMP-Collagen composite in calvarial subperiosteum of matured rats.
- Microbeam X-ray Diffraction Analysis of Human Tooth Enamel from EDJ to Enamel Surface
- Basement Membrane-like Structures of Oral Invasive Melanoma : An Immunohistochemical Study Using Type IV Collagen Alpha Chains(Asian Science Seminar/III-4, The 3^ Asian Science Seminar)
- Characteristics of Melanosomes in Melanotic and Amelanotic Melanomas
- Localization of CD31, CD34, and CD105 as a Measure of Microvessel Density in Cervix Neoplasms(Asian Science Seminar/III-3, The 3^ Asian Science Seminar)
- Frequent Deletion and Down-Regulation of ING3 in Head and Neck Cancer(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
- Wnt5a Participates in the Mouse Embryonic Maxillary-Mandible Development(Asian Science Seminar/I-3, The 3^ Asian Science Seminar)
- 12. Gene expressions of bone and cartilage matrix proteins during ectopic hard tissue formation induced by BMP.
- 14. Ultrastructure of initial calcification in BMP-collagen induced bone.
- Biological Analysis of a Candidate Stem Cell-KUSA/A1 cell-for Bone Tissue Engineering
- Chemical Analysis of a Novel Coating Material, CaTiO_3-aC
- Expression and mutation analysis of epidermal growth factor receptor in head and neck squamous cell carcinoma
- Downregulation of ING3 mRNA expression predicts poor prognosis in head and neck cancer
- Histological and Morphometrical Study of Bone Formation in Rat Subcutaneous Tissue by BMP-Collagen Particles
- Wnt5a Gene Plays a Role in Mouse Embryonic Orofacial Development(International Symposium of Maxillofacial & Oral Regenerative Biology in OKAYAMA 2005)
- In Vivo Effect of Fluoride-substituted Apatite on Rat Bone
- Effect of Magnesium, Strontium or Fluoride Ions on in vitro Activities of Odontoblast-like Cells (MDPC-23)
- Pheomelanin and Eumelanin of Malignant Melanomas and Melanosis in Oral Mucosa
- Localization of Oxytalan fiber, Type III Collagen and BMP Family in Conventional and Desmoplastic Ameloblastoma
- Characterization and Gene Expression Profiling of Human Mesenchymal Stem Cells
- Preparation and Characterization of Calcium Silicate/Vermiculite Composite
- Hepatotoxicity Induced by Excessive Intake of Rhubarb
- Effect of Rhubarb on Heat Shock Protein 70 Expression in Lung Tissues from Rats with Acute Lung Injury Induced by Lipopolysaccharide
- Angiogenic Squamous Dysplasia in Oral Epithelial Dysplastic Lesions(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
- Oral Potentially Malignant Epithelial Lesions and Associated Risk in the Contralateral Mucosa(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
- Analysis of Amelogenin Gene (AMGX, AMGY) Expression in Ameloblastoma(Asian Science Seminar/III-1, The 3^ Asian Science Seminar)
- Immunohistochemical study of BMP family in odontogenic tumors(2nd ASIAN SCIENCE SEMINAR IN OKAYAMA 2003)
- Inhibitor of Growth (ING) Family : An Emerging Molecular Target for Cancer Therapy
- BMP Family Play Regulatory Roles in Determining the Shape Development of Odontogenic Tumors(Asian Science Seminar/I-4, The 3^ Asian Science Seminar)
- Long-term Observations of Heterotopic Bone Formation Induced by rhBMP-2 and IBM Composites
- Gene Expression of Chondro-Osseous Cells in Heterotopic Bone formation Induced by rhBMP-2
- 15. Natural bovine bone morphogenetic protein (BMP) induces cytological and functional differentiation of preodontoblasts in vitro.
- Mechanism of new bone formation by using immature stem cell KUSA/A1 for bone tissue engineering in vivo study(5th Asian Science Seminar,Session 1)
- Biological analysis of bone marrow stromal cells in vivo cell culture method(5th Asian Science Seminar,Session 1)
- Immunohistochemical studies of a case of clear cell chondrosarcoma
- 8. Type I, II, and X Collagen Gene Expression in Osteosclerotic(oc/oc)Mouse Femur(8th Annual Meeting of the Society of Hard Tissue Biology July 24, 1999 in Asahikawa)
- Mutation analysis of the EGFR pathway in head and neck squamous cell cancer(5th Asian Science Seminar,Session 1)
- Epigenetic and Genetic Modifications of BRG1, A Candidate Tumor Suppressor Gene in Oral Cancer(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
- Loss of Heterozygosity Analysis of Chromosomal 2q22-37 Region in Oral Cancers(Asian Science Seminar/III-2, The 3^ Asian Science Seminar)
- Principles of Electron Spectroscopic Imaging(ESI)in Hard Tissue Biology
- The Present State of Diagnosis and Practice in Surgical Pathology of Dental Medicine : A report on a questionnaire investigation
- Micro X-ray Diffraction Study of Carious Tooth Enamel
- Augmentation of Peri-implant Bone Defects with Different Bone Grafts and Guided Bone Regeneration : A Pilot Experimental Study in the Dog
- Expression of Hsp70 in Acute Lung Injury Induced by Lipopolysaccharide
- Crystallographic Properties and Cell Affinity Study of Synthetic α-Tricalcium Phosphate, β-Tricalcium Phosphate, Tetracalcium Phosphate and Fluorapatite
- Demonstration of Calcium Ion Distribution in Calcifying Cells : X-ray Microanalysis and Electron Spectroscopic Imaging after Fixation with NHA-Containing Fixative and Microwave Irradiation
- Initial Calcification of Bone Formation in Ectopic and Osteogenic Tissue Induced by BMP-Collagen Composites(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
- Effects of Bovine Amelogenin and Enamelin on in vitro Mineralization in Gel
- Influence of Three-Dimensional Scaffold on Bone Induction by KUSA/A1 Cells
- 19. Gene expression TypeI, II, and X Collagen during Ectopic Bone Formation Induced by Bone Morphogenetic.
- The Effect of BMP on the Monocyte-Macrophage-Mesenchymal Cell Interaction in Ectopic Bone Formation
- Phospholipids in Amelogenesis and Dentinogenesis
- Detecting the (Epidermal Growth Factor Receptor) EGFR Gene Amplification in Oral Carcinogenesis(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
- Immunohistochemical Study of Notch Signaling Proteins in the Calcifying Epithelial Odontogenic Tumor (Pindborg Tumor)
- The Effect of Calcium Hydroxide Removal and the Influence on the Sealing Ability of Root Canals
- Immunohistochemical Observation of Notch Signaling in a Case of Calcifying Cystic Odontogenic Tumor
- WS1-1 Practice of Immunohistochemistry and in situ hybridization in hard tissue research(THE 45TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)