Histopathological Study of Matrix Mineralization by Osteoblastic-like and Odontoblastic-like Cells in Diffusion Chamber
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概要
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Bone and dentin resemble each other in composition and mechanism of formation and mineralization. Preliminary analysis of osteoblastic and odontoblastic cells before using them in tissue engineering is mandatory. Thus, in order to evaluate the process of calcification, we evaluated osteoblastic-like (KUSA/A1cells) and odontoblastic-like (MDPC-23 cells) cells seeded in cell culture and in intraperitoneal diffusion chamber. Our results indicated that KUSA/A1 cells differentiated into osteoblasts-like cells and induced bone tissue inside the chamber. Whereas, MDPC-23 cells showed odontoblastic phenotype without ability to induce dentin formation, suggesting that MDPC-23 cells are special cells, which lost the capacity to induce mineralized dentin matrix after long period of time. This study showed the significance of basic information of calcification process by osteoblastic-like and odontoblastic-like cells before using them in tissue engineering.
著者
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Xiao Jing
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry & Pharmaceutical S
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Katase Naoki
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry & Pharmaceutical S
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Rodriguez Andrea
Department of Oral Pathology and Medicine, Graduate School of Medicine and Dentistry, Okayama Univer
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Nakano Keisuke
Department of Oral Pathology and Medicine, Graduate School of Medicine and Dentistry, Okayama Univer
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Hidetsugu Tsujigiwa
Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni
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Tsujigiwa Hidetsugu
徳島文理大学 薬学部機能形態学
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Tsujigiwa Hidetsugu
Department Of Oral Pathology And Medicine Graduate School Of Medicine & Dentistry Okayama Univer
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Xiao Jing
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry & Pharmaceutical
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Xiao Jing
Department Of Oral Biology College Of Stomatology Dalian Medical University
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Lee You-jin
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Ara Sathi
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Katase Naoki
Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A
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Nakano Keisuke
Hard Tissue Pathology Unit Matsumoto Dental University Graduate School Of Oral Medicine
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Lee You-jin
Department Of Electronics And Computer Engineering Hanyang University
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Takagi Tohru
Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A
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Takagi Tohru
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Takagi Tohru
Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni
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Andrea Rodriguez
Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A
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Rodriguez Andrea
Department Of Oral Pathology Dental School Tucuman University:department Of Oral Pathology And Medic
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Borkosky Silvia
Department of Oral Pathology, Tucuman National University, School of Dentistry
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Han Phuu
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical
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Okauchi Mika
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical
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Xiao Jing
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Ouchida Mamoru
Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A
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Han Phuu
Orofacial Pain And Oral Medicine Center Division Of Diagnostic Sciences School Of Dentistry Universi
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Borkosky Silvia
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Tujigiwa Hidetugu
Department Of Oral Pathology And Medicine Graduate School Of Medicine And Dentistry Okayama Universi
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Tsujigiwa Hidetsugu
Department Of Oral Pathology And Medicine Graduate School Of Medicine And Dentistry. Okayama Univers
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Okauchi Mika
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Xiao Jing
Department Of Oral Pathology College Of Stomatology Dalian Medical University:department Of Oral Pat
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Xiao Jing
Department Of Oral Pathology College Of Stomatology Dalian Medical University:department Of Oral Pat
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Nakano Keisuke
Department Of Information Engieering Niigata University
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Katase Naoki
Departments Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical
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Rodriguez Andrea
Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni
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Rodriguez Andrea
Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A
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Katase Naoki
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Takagi Tohru
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Takagi Tohru
Department Of Biochemistry Faculty Of Dentistry Tokyo Medical And Dental University
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Tujigiwa Hidetugu
Department Of Oral Pathology And Medicine Graduate School Of Medicine And Dentistry. Okayama Univers
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Takagi Tohru
Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A
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Tujigiwa Hidetugu
Dept. Of Oral Pathology Okayama Univ. Dental School.
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Takagi Tohru
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Takagi Tohru
Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni
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Takagi Tohru
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Okauchi Miho
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Takagi Tohru
Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni
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Takagi Tohru
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Rodrigez Andrea
Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A
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Nakano Keisuke
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Tsujigiwa Hidetsugu
Department Of Oral Pathology School Of Dentistry Okayama University
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Rodriguez Andrea
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Rodriguez Andrea
Department Of Oral Pathology And Medicine Graduate School Of Medicine And Dentistry Okayama University
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Lee You-Jin
Department of Electronic Engineering, Hanyang University, Seoul 133-791, Korea
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