Effects of Immobilized rhBMP-2/atelocollagen in vivo and in vitro
スポンサーリンク
概要
- 論文の詳細を見る
The use of recombinant human bone morphogenetic protein -2 (rhBMP-2) to induce ectopic bone formation requires a carrier. Atelocollagen, a biomaterial with a porous structure, excellent operational features and biocompatibility, is known to be an effective carrier for rhBMP-2. However, conventionally used lyophilized rhBMP-2/atelocollagen mixture does not necessarily give adequate bone induction effect. In the present study, we evaluated the effect of immobilizing rhBMP-2 to succinylated type I atelocollagen on the cellular activity of ST2 cells and immobilized rhBMP-2/atelocollagen and non immobilized rhBMP-2/atelocollagen implanted in subcutaneous pockets of Wister rats. Our results revealed that 1) Alkaline phosphatase activity confirmed the effectiveness of rhBMP-2/succinylated type I atelocollagen immobilization in augmenting cellular activity. 2) Intracellular signaling continued for prolonged period when rhBMP-2 was immobilized to succinylated type I atelocollagen. 3) In rhBMP-2/atelocollagen implants were completely fully with new bone formation and cell proliferation. Whereas, in non immobilized rhBMP-2/atelocollagen implants showed new hard tissue in the periphery of the carrier with only collagen membrane in its center. This study indicated that immobilizing rhBMP-2 is an efficient method to increase bone induction.
- 硬組織再生生物学会の論文
著者
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Xiao Jing
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry & Pharmaceutical S
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Rodriguez Andrea
Department of Oral Pathology and Medicine, Graduate School of Medicine and Dentistry, Okayama Univer
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Hidetsugu Tsujigiwa
Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni
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Tsujigiwa Hidetsugu
徳島文理大学 薬学部機能形態学
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Tsujigiwa Hidetsugu
Department Of Oral Pathology And Medicine Graduate School Of Medicine & Dentistry Okayama Univer
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Xiao Jing
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry & Pharmaceutical
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Xiao Jing
Department Of Oral Biology College Of Stomatology Dalian Medical University
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Li Xie
Harbin Medical University Faculty Of Dentistry
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Takagi Tohru
Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A
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Takagi Tohru
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Takagi Tohru
Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni
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Andrea Rodriguez
Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A
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Rodriguez Andrea
Department Of Oral Pathology Dental School Tucuman University:department Of Oral Pathology And Medic
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Long Hu
Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni
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Kan Rui
Department of Virology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama
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Lu Zhenfu
China Medical University, Faculty of Dentistry
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Gao Wen-Xin
Ji Lin University, Faculty of Dentistry
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Tan Jin
Dalian Medical University, Faculty of dentistry
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Xiao Jing
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Tujigiwa Hidetugu
Department Of Oral Pathology And Medicine Graduate School Of Medicine And Dentistry Okayama Universi
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Tsujigiwa Hidetsugu
Department Of Oral Pathology And Medicine Graduate School Of Medicine And Dentistry. Okayama Univers
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Kan Rui
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Kan Rui
Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni
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Gao Wen-xin
Ji Lin University Faculty Of Dentistry
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Xiao Jing
Department Of Oral Pathology College Of Stomatology Dalian Medical University:department Of Oral Pat
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Xiao Jing
Department Of Oral Pathology College Of Stomatology Dalian Medical University:department Of Oral Pat
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Tan Jin
Dalian Medical University Faculty Of Dentistry
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Lu Zhenfu
China Medical University
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Lu Zhenfu
China Medical University Faculty Of Dentistry
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Rodriguez Andrea
Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni
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Rodriguez Andrea
Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A
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Takagi Tohru
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Takagi Tohru
Department Of Biochemistry Faculty Of Dentistry Tokyo Medical And Dental University
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Tujigiwa Hidetugu
Department Of Oral Pathology And Medicine Graduate School Of Medicine And Dentistry. Okayama Univers
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Tujigiwa Hidetugu
Dept. Of Oral Pathology Okayama Univ. Dental School.
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Takagi Tohru
Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni
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Takagi Tohru
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Takagi Tohru
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Tsujigiwa Hidetsugu
Department Of Oral Pathology School Of Dentistry Okayama University
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Rodriguez Andrea
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Rodriguez Andrea
Department Of Oral Pathology And Medicine Graduate School Of Medicine And Dentistry Okayama University
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