In Vitro Studies on Ameloblast Cells and Mineralization

概要

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Unerupted teeth were extracted from the mandibles of calves, and the enamel surfaces containing ameloblasts and undifferentiated cementoblasts like cells were scrapped off. Cells migrated from the fragments were trunsferred into cultrre flasks containing small bars of sintered hydroxyapatite, and the cultures were incubated long term. The most of the cells showed a fibroblastic morphology, but some of the cells formed an epithelial cell nest. The fibroblastic cells grew steadily, but the epithelial cell nest showed poor growth. The fibroblastic cells formed multiple cell layers, and were embedded in collagenous matrix. By 3 months, fibrous bands appeared on the surfacc of the cell layers and surrounded the bars of sintered hydroxyapatite. Such bands increased in size with incubation time. Deposition of calcified substances was observed in the well developed bands. The deposited crystals were found by the X ray powder diffraction method to be low crystalline hydroxyapatite; the Ca/P molar ratio of 1.49 was determined by the ICP method.
硬組織再生生物学会の論文