Improvement of Adhesion and Spreading of Human Gingival Fibroblasts to RGD-grafted Titanium Surface
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概要
- 論文の詳細を見る
Objective: Arg-Gly-Asp(RGD)-containing peptide was coupled on titanium surface of dental implants. It is confirmed that this peptide promotes human gingival fibroblasts (HGFs) adhesion during the early stage after implantation was tested. Methods: RGD peptides were attached covalently to the titanium surface by 1, 1'-carbonyldiimidazole (CDI). Primary HGFs were inoculated on commercially pure titanium and RGD-grafted titanium respectively. Cell attachment and proliferation was assessed by acridine orange staining technology. Cell spreading were checked by immunofluorescence staining and confocal laser scanning microscope. Results: The number of attached and proliferated HGFs and their average spreading area were higher on RGD modified titanium than on unmodified titanium. The actin cytoskeleton reorganization existed earlier on RGD coated samples than on uncoated ones. Conclusions: This research may develop a new potential method to enhance the integration between human gingival soft tissue and titanium implant surfaces.
- 硬組織再生生物学会の論文
著者
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Nagai Noriyuki
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry & Pharmaceutical S
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Takagi Tohru
Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A
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Takagi Tohru
Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni
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Kawakami Toshiyuki
Hard Tissue Pathology Unit, Matsumoto Dental University Graduate School of Oral Medicine
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Hu Hailong
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Zhao Baohong
School of Stomatology, China Medical University
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Yi Zhe
School of Stomatology, China Medical University
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Lu Zhenfu
School of Stomatology, China Medical University
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Tian Weiming
Department of Materials Science & Engineering, Tsinghua University
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Cui Fuzhai
Department of Materials Science & Engineering, Tsinghua University
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Feng Hailan
School of Stomatology, Peking University
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Hu Hailong
School of Stomatology, China Medical University
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Huang Hai-tao
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Nagai Noriyuki
Department Of Oral Pathology Okayama University Dental School
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Nagai Noriyuki
Department Of Oral Pathology And Medicine Dental School Okayama University
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Kawakami Toshiyuki
Hard Tissue Unit Matsumoto Dental University Graduate School Of Oral Medicine
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Kawakami Toshiyuki
Hard Tissue Pathology Unit Matsumoto Dental University Graduate School Of Oral Medicine
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Haillong Hu
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Yi Zhe
School Of Stomatology China Medical University
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Lu Zhenfu
School Of Stomatology China Medical University
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Cui Fuzhai
Department Of Materials Science & Engineering Tsinghua University
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Feng Hailan
School Of Stomatology Peking University
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Tian Weiming
Department Of Materials Science & Engineering Tsinghua University
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Zhao Baohong
School Of Stomatology China Medical University
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Takagi Tohru
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Takagi Tohru
Department Of Biochemistry Faculty Of Dentistry Tokyo Medical And Dental University
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Nagai Noriyuki
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Hu Hailong
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Haillong Hu
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
-
Nagai Noriyuki
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
-
Takagi Tohru
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
-
Takagi Tohru
Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni
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Takagi Tohru
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Kawakami Toshiyuki
Hard Tissue Pathology Matsumoto Dental University Institute For Dental Science
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Takagi Tohru
Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S
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Baohong Zhao
School of Stomatology, China Medical University
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Zhenfu Lu
School of Stomatology, China Medical University
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Nagai Noriyuki
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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