Histopathological and Immunohistochemical Study of Heterotopic Chondro-Osseous Tissue Formation Induced by S-200 BMP

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The composite of S-200 bone morphogenetic protein (S-200 BMP) and fibrous glass membrane (FGM) was implanted in the dorsal subcutaneous tissues of rats, and the tissues were examined histopathologically and immunohistochemically at 1, 2 and 3 weeks after implantation. At 1 week after implantation, the explants showed a three zone structure which included the inner zone mainly consisting of FGM, the intermediate zone of chondro-osseous tissue containing collagen type II in the matrix, and the peripheral zone of connective tissue containing collagen type I. At. 2 weeks, osteocalcin was detected in the hypertrophic chondroblast-like cells in the chondro-osseous tissue, a phenomenon which does not exist in the normal endochondral ossification mode of long bones. At 2 weeks, collagen type I positive eosinophilic chondro-osseous tissue without collagen II was present between the basophilic chondro-osseous tissue containing collagen II, osteocalcin and the woven bone mingled with connective tissue at the periphery. At 3 weeks, bone morrow was formed and collagen type II still remained in the chondro-osseous tissue. Our findings indicate that S-200 BMP/FGM induces the chondro-osseous tissue through a process like that in the endochondral ossification mode but the matrix components and cell differentiation patterns dirrer from those in the nornrai fetal endochondral ossification process.
硬組織再生生物学会の論文